Gene/Protein Characteristic Table for KIAA0550
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Product ID ORK00559
Accession No AB011122
Description adhesion G protein-coupled receptor B3
Clone name hh15909
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5420 bp)
Predicted protein sequence (1524 aa)
Flexi ORF Clone FXC00559
Source Human adult brain
Rouge ID mKIAA0550 by Kazusa Mouse cDNA Project
Note We replaced hh00851, former representative clones for KIAA0550 with hh15909. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5420 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 614 bp
Genome contig ID gi89161210f_69302564
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
AAAATGTTGTATGGTGTAAATAAACTTTTGTCTAC
Flanking genome sequence
(853556 - 853605)
----+----*----+----*----+----*----+----*----+----*
ATATCAGTTTTTTAGTGCATTTTATTTTGGATTTGTTGACCAGAAATTTA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 6 f 69402564 70156118 32 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 1524 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O60242 0 100.0 Brain-specific ...
Homo sapiens
CAH73864 0 99.9 brain-specific ...
Homo sapiens
BAF82385 0 99.8 unnamed protein...
Homo sapiens
XP_001503435 0 98.7 similar to Brai...
Equus caballus
XP_538991 0 98.5 similar to Brai...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB018311 2.8e-25 22.8 KIAA0768
AB018329 5.7e-25 26.1 KIAA0786
AB020628 4.7e-24 25.5 KIAA0821
AB040878 1.6e-20 31.9 KIAA1445
D87469 3.6e-20 24.8 KIAA0279
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR008077 57 74 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 539 551 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 559 575 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 616 635 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 658 667 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 719 740 PR01694 Brain-specific angiogenesis inhibitor
IPR000832 881 905 PR00249 GPCR
IPR008077 905 915 PR01694 Brain-specific angiogenesis inhibitor
IPR000832 945 968 PR00249 GPCR
IPR008077 969 982 PR01694 Brain-specific angiogenesis inhibitor
IPR000832 982 1007 PR00249 GPCR
IPR008077 1008 1025 PR01694 Brain-specific angiogenesis inhibitor
IPR000832 1025 1050 PR00249 GPCR
IPR000832 1100 1120 PR00249 GPCR
IPR008077 1121 1131 PR01694 Brain-specific angiogenesis inhibitor
IPR000832 1131 1152 PR00249 GPCR
IPR008077 1185 1198 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 1413 1427 PR01694 Brain-specific angiogenesis inhibitor
IPR008077 1430 1444 PR01694 Brain-specific angiogenesis inhibitor
HMMPfam IPR000884 297 344 PF00090 Thrombospondin
IPR000884 351 399 PF00090 Thrombospondin
IPR000884 406 454 PF00090 Thrombospondin
IPR000884 461 509 PF00090 Thrombospondin
IPR001879 513 574 PF02793 Hormone receptor
IPR000203 817 865 PF01825 GPS
IPR000832 876 1156 PF00002 GPCR
HMMSmart IPR000884 296 345 SM00209 Thrombospondin
IPR000884 350 400 SM00209 Thrombospondin
IPR000884 405 455 SM00209 Thrombospondin
IPR000884 460 510 SM00209 Thrombospondin
IPR001879 512 578 SM00008 Hormone receptor
IPR000203 817 870 SM00303 GPS
ProfileScan IPR000859 32 161 PS01180 CUB
IPR000884 293 345 PS50092 Thrombospondin
IPR000884 347 400 PS50092 Thrombospondin
IPR000884 402 455 PS50092 Thrombospondin
IPR000884 457 510 PS50092 Thrombospondin
IPR001879 493 573 PS50227 Hormone receptor
IPR000203 818 870 PS50221 GPS
IPR000832 879 1153 PS50261 GPCR
ScanRegExp IPR000832 1141 1156 PS00650 GPCR

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 5 AVRNLLIYIFSTYLLVMFGFNAA 27 PRIMARY 23
2 881 VTLIVGSGLSCLALITLAVVYAA 903 PRIMARY 23
3 912 RSIILINFCLSIISSNILILVG 933 SECONDARY 22
4 943 CTTTTAFLHFFFLASFCWVLTEA 965 SECONDARY 23
5 985 FLCLGWGLPALVVATSVGF 1003 SECONDARY 19
6 1025 YAFVGPAAAVVLVNMVIGILVFN 1047 PRIMARY 23
7 1101 LWSSCVVLPLLALTWMSAVLAMT 1123 PRIMARY 23
8 1130 FQILFAVFDSLQGFVIVMVHCIL 1152 PRIMARY 23
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f ATCATTTCACATACCTCCTGC
Primer_r GGATAAGGATGAGGAGGGGAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name GeneBridge 4
Primer_f ATCATTTCACATACCTCCTGC
Primer_r GGATAAGGATGAGGAGGGGAC
PCR product length 127 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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