##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI ApaI AscI Asp718I
AvaI AviII AvrII BbeI BbrPI
BciVI BcnI BlnI BsaAI BsaI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BsrBI BssHII BssSI BstBI
BstUI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I FseI FspI
HaeII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NarI NciI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 3525 GGCGTC G[GA]^CG[CT]C
AflII 4855 CTTAAG C^TTAAG
AhdI 859 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1515 GTGCAC G^TGCAC
ApaLI 1515 GTGCAC G^TGCAC
AseI 2584 ATTAAT AT^TAAT
AsnI 2584 ATTAAT AT^TAAT
AspEI 859 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4120 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3735 GGATCC G^GATCC
BanI 1864 GGCACC G^G[CT][GA]CC
BanII 3537 GAGCTC G[GA]GC[CT]^C
BbsI 1892 GTCTTC GAAGAC(2/6)
BbvCI 832 CCTCAGC CCTCAGC(-5/-2)
BcgI 3748 GCAGATTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4855 CTTAAG C^TTAAG
BglI 2815 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2547 AGATCT A^GATCT
BpuAI 1892 GTCTTC GAAGAC(2/6)
BsaBI 1934 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3525 GGCGTC G[GA]^CG[CT]C
BsaWI 2478 TCCGGT [AT]^CCGG[AT]
BseRI 1358 CTCCTC GAGGAG(10/8)
Bst1107I 813 GTATAC GTA^TAC
BstXI 4735 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 813 GTATAC GTA^TAC
CfoI 2525 GCGC GCG^C
DrdI 3570 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 859 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4455 GATATC GAT^ATC
HhaI 2525 GCGC GCG^C
Hin1I 3525 GGCGTC G[GA]^CG[CT]C
HinP1I 2525 GCGC G^CGC
MamI 1934 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1508 GCCGGC GCC^GGC
NgoMIV 1508 GCCGGC G^CCGGC
PflFI 4120 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1429 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2584 ATTAAT AT^TAAT
SacI 3537 GAGCTC GAGCT^C
SexAI 4984 ACCAGGT A^CC[AT]GGT
SpeI 4960 ACTAGT A^CTAGT
SphI 3777 GCATGC GCATG^C
StuI 2614 AGGCCT AGG^CCT
Tth111I 4120 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1429 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1141 ACATGT A^C[GA][CT]GT
1315 ACATGT
AocI 1063 CCTCAGG CC^T[ACGT]AGG
1780 CCTGAGG
BclI 507 TGATCA T^GATCA
3264 TGATCA
BpmI 1817 CTCCAG CTGGAG(16/14)
2644 CTGGAG
BsmBI 2800 CGTCTC CGTCTC(1/5)
3527 CGTCTC
Bsp1407I 1591 TGTACA T^GTACA
4990 TGTACA
BspHI 1047 TCATGA T^CATGA
3788 TCATGA
BspLU11I 1141 ACATGT A^CATGT
1315 ACATGT
BsrFI 1508 GCCGGC [GA]^CCGG[CT]
3421 GCCGGT
BsrGI 1591 TGTACA T^GTACA
4990 TGTACA
BstEII 1289 GGTCACC G^GT[ACGT]ACC
2028 GGTGACC
BstPI 1289 GGTCACC G^GT[ACGT]ACC
2028 GGTGACC
Bsu36I 1063 CCTCAGG CC^T[ACGT]AGG
1780 CCTGAGG
Cfr10I 1508 GCCGGC [GA]^CCGG[CT]
3421 GCCGGT
Eco81I 1063 CCTCAGG CC^T[ACGT]AGG
1780 CCTGAGG
EcoNI 924 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1983 CCTTGCTAAGG
EcoO65I 1289 GGTCACC G^GT[ACGT]ACC
2028 GGTGACC
EcoRI 2316 GAATTC G^AATTC
4279 GAATTC
FauI 4624 CCCGC CCCGC(4/6)
4674 CCCGC
FbaI 507 TGATCA T^GATCA
3264 TGATCA
HincII 2422 GTTGAC GT[CT]^[GA]AC
3340 GTCAAC
HindII 2422 GTTGAC GT[CT]^[GA]AC
3340 GTCAAC
HindIII 2359 AAGCTT A^AGCTT
5351 AAGCTT
RcaI 1047 TCATGA T^CATGA
3788 TCATGA
SspBI 1591 TGTACA T^GTACA
4990 TGTACA
XbaI 2193 TCTAGA T^CTAGA
5335 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BbvI BfaI BlpI
BmyI Bpu1102I BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI