OPN4 ORF clone pFN21AE9448

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9448

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Clone info.

Species

human

Clone Name

pFN21AE9448 Sequence (with vector) Restriction Map

Product ID

FHC31452

ORF Sequence

DNA sequence (1434 bp)
Protein sequence (478 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9448

Symbol

OPN4

Alias : MOP

Description

opsin 4, transcript variant 1

Original Clone ID

cp99448

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1434 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 478 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7376.1 2.6e-138 99.8 OPN4 CCDS31237.1 9.7e-110 97.5 OPN4 CCDS4923.1 7.9e-19 30.8 OPN5 CCDS3687.1 1.2e-14 30.9 RRH CCDS3063.1 8.4e-13 28.7 RHO The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 72 96 PR00237 GPCR IPR001760 93 105 PR00238 Opsin IPR000276 105 126 PR00237 GPCR IPR000276 150 172 PR00237 GPCR IPR000276 186 207 PR00237 GPCR IPR001760 208 220 PR00238 Opsin IPR000276 236 259 PR00237 GPCR IPR000276 294 318 PR00237 GPCR IPR001760 329 341 PR00238 Opsin IPR000276 332 358 PR00237 GPCR HMMPfam IPR000276 87 350 PF00001 GPCR ProfileScan IPR017452 87 350 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 42 LPSISPTAPGTWAAAWVPLPTVD 64 SECONDARY 23 2 73 LGTVILLVGLTGMLGNLTVIYTF 95 SECONDARY 23 3 117 FLMSFTQAPVFFTSSLYKQWLFG 139 SECONDARY 23 4 150 GALFGISSMITLTAIALDRYLVI 172 SECONDARY 23 5 187 AAFVLLGVWLYALAWSLPPFFGW 209 PRIMARY 23 6 238 LLCCFVFFLPLLIIIYCYIFIFR 260 PRIMARY 23 7 298 MLLVILLFVLSWAPYSAVALVAF 320 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_150598 478 606665 99.8 100.0 XP_016872445 528 606665 99.4 98.1 NP_001025186 489 606665 97.5 100.0 XP_016872446 405 606665 97.3 82.4 XP_016872444 539 606665 97.1 98.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9448 Download

>KIEE9448  1434 bp
ATGAACCCTCCTTCGGGGCCAAGAGTCCTGCCCAGCCCAACCCAAGAGCCCAGCTGCATG
GCCACCCCAGCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGC
CGGCTTCCATCCATCAGTCCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTC
CCCACGGTTGATGTTCCAGACCATGCCCACTATACCCTGGGCACAGTGATCTTGCTGGTG
GGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATACCTTCTGCAGGAGCAGAAGC
CTCCGGACACCTGCCAACATGTTCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCC
TTCACCCAGGCCCCTGTCTTCTTCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAG
ACAGGCTGCGAGTTCTATGCCTTCTGTGGAGCTCTCTTTGGCATTTCCTCCATGATCACC
CTGACGGCCATCGCCCTGGACCGCTACCTGGTAATCACACGCCCGCTGGCCACCTTTGGT
GTGGCGTCCAAGAGGCGTGCGGCATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTGGCC
TGGAGTCTGCCACCCTTCTTCGGCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCC
TGCTCCTGGGACTACATGAGCTTCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGC
TGCTTCGTGTTCTTCCTCCCTCTGCTTATCATCATCTACTGCTACATCTTCATCTTCAGG
GCCATCCGGGAGACAGGACGGGCTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAG
TCCCTGTGGCAGCGGCAGCGGCTGCAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTG
GTCATCCTCCTCTTCGTGCTCTCCTGGGCTCCCTATTCCGCTGTGGCCCTGGTGGCCTTT
GCTGGGTACGCACACGTCCTGACACCCTACATGAGCTCGGTGCCAGCCGTCATCGCCAAG
GCCTCTGCAATCCACAACCCCATCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCC
ATTGCCCAGCACCTGCCCTGCCTGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGC
CCCTACCCCAGCTACCGCTCCACCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTC
AGCTGGATCTCCATACGGAGGCGCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGG
ACACACATGGAGGCAGCAGCTGTGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTC
TACGGTCAGGGTCTGGAGGACTTGGAAGCCAAGGCACCCCCCAGACCCCAGGGACACGAA
GCAGAGACTCCAGGGAAGACCAAGGGGCTGATCCCCAGCCAGGACCCCAGGATG

ORF Protein

Cloned ORF protein sequence for pFN21AE9448 Download

>KIEE9448  478 aa
MNPPSGPRVLPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPTAPGTWAAAWVPL
PTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCRSRSLRTPANMFIINLAVSDFLMS
FTQAPVFFTSSLYKQWLFGETGCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFG
VASKRRAAFVLLGVWLYALAWSLPPFFGWSAYVPEGLLTSCSWDYMSFTPAVRAYTMLLC
CFVFFLPLLIIIYCYIFIFRAIRETGRALQTFGACKGNGESLWQRQRLQSECKMAKIMLL
VILLFVLSWAPYSAVALVAFAGYAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYRVA
IAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNLSWISIRRRQESLGSESEVGW
THMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGKTKGLIPSQDPRM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9448 Download

>pFN21AE9448  6139 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACCCTCCTTCGGGGCCAAGAGTCCTGCCCAG
CCCAACCCAAGAGCCCAGCTGCATGGCCACCCCAGCACCACCCAGCTGGTGGGACAGCTC
CCAGAGCAGCATCTCCAGCCTGGGCCGGCTTCCATCCATCAGTCCCACAGCACCTGGGAC
TTGGGCTGCTGCCTGGGTCCCCCTCCCCACGGTTGATGTTCCAGACCATGCCCACTATAC
CCTGGGCACAGTGATCTTGCTGGTGGGACTCACGGGGATGCTGGGCAACCTGACGGTCAT
CTATACCTTCTGCAGGAGCAGAAGCCTCCGGACACCTGCCAACATGTTCATTATCAACCT
CGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTCTTCACCAGTAGCCT
CTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCTTCTGTGGAGCTCT
CTTTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACCGCTACCTGGTAAT
CACACGCCCGCTGGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGGCATTTGTCCTGCT
GGGCGTTTGGCTCTATGCCCTGGCCTGGAGTCTGCCACCCTTCTTCGGCTGGAGCGCCTA
CGTGCCCGAGGGGTTGCTGACATCCTGCTCCTGGGACTACATGAGCTTCACGCCGGCCGT
GCGTGCCTACACCATGCTTCTCTGCTGCTTCGTGTTCTTCCTCCCTCTGCTTATCATCAT
CTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACAGGACGGGCTCTCCAGACCTT
CGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGCTGCAGAGCGAGTG
CAAGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCTCCTGGGCTCCCTA
TTCCGCTGTGGCCCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGACACCCTACATGAG
CTCGGTGCCAGCCGTCATCGCCAAGGCCTCTGCAATCCACAACCCCATCATTTACGCCAT
CACCCACCCCAAGTACAGGGTGGCCATTGCCCAGCACCTGCCCTGCCTGGGGGTGCTGCT
GGGTGTATCACGCCGGCACAGTCGCCCCTACCCCAGCTACCGCTCCACCCACCGCTCCAC
GCTGACCAGCCACACCTCCAACCTCAGCTGGATCTCCATACGGAGGCGCCAGGAGTCCCT
GGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCAGCTGTGTGGGGAGCTGC
CCAGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACTTGGAAGCCAAGGC
ACCCCCCAGACCCCAGGGACACGAAGCAGAGACTCCAGGGAAGACCAAGGGGCTGATCCC
CAGCCAGGACCCCAGGATGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT