(Linear) (Six-base) MAPSORT of: KIEE9448.seq Check: 8725 from: 1 to: 1434 KIEE9448.orf With 182 enzymes: SgfI * March 22, 2012 18:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 318 1100 1434 Size: 318 782 334 Fragments arranged by size: 782 334 318 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 759 1434 Size: 759 675 AflIII A'CryG_T Cuts at: 0 317 972 1434 Size: 317 655 462 Fragments arranged by size: 655 462 317 AlwNI CAG_nnn'CTG Cuts at: 0 148 861 1092 1279 1332 1434 Size: 148 713 231 187 53 102 Fragments arranged by size: 713 231 187 148 102 53 AvaI C'yCGr_G Cuts at: 0 640 1434 Size: 640 794 BanI G'GyrC_C Cuts at: 0 999 1220 1353 1434 Size: 999 221 133 81 Fragments arranged by size: 999 221 133 81 BanII G_rGCy'C Cuts at: 0 50 453 804 930 997 1240 1434 Size: 50 403 351 126 67 243 194 Fragments arranged by size: 403 351 243 194 126 67 50 BbeI G_GCGC'C Cuts at: 0 1224 1434 Size: 1224 210 BbsI GAAGACnn'nnnn_ Cuts at: 0 369 1402 1434 Size: 369 1033 32 Fragments arranged by size: 1033 369 32 BbvCI CC'TCA_GC Cuts at: 0 1198 1434 Size: 1198 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 418 452 1434 Size: 418 34 982 Fragments arranged by size: 982 418 34 BclI T'GATC_A Cuts at: 0 473 1434 Size: 473 961 BglI GCCn_nnn'nGGC Cuts at: 0 952 1434 Size: 952 482 Bme1580I G_kGCm'C Cuts at: 0 223 641 1434 Size: 223 418 793 Fragments arranged by size: 793 418 223 BmgBI CAC'GTC Cuts at: 0 975 1434 Size: 975 459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 621 645 790 1354 1373 1434 Size: 93 528 24 145 564 19 61 Fragments arranged by size: 564 528 145 93 61 24 19 Bpu10I CC'TnA_GC Cuts at: 0 1198 1434 Size: 1198 236 BsaAI yAC'GTr Cuts at: 0 636 1434 Size: 636 798 BsaHI Gr'CG_yC Cuts at: 0 544 1221 1434 Size: 544 677 213 Fragments arranged by size: 677 544 213 BsaWI w'CCGG_w Cuts at: 0 302 1434 Size: 302 1132 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 788 818 1434 Size: 788 30 616 Fragments arranged by size: 788 616 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 898 1434 Size: 898 536 BseYI C'CCAG_C Cuts at: 0 31 49 66 76 255 573 961 1085 Size: 31 18 17 10 179 318 388 124 Cuts at: 1085 1113 1147 1295 1414 1434 Size: 28 34 148 119 20 Fragments arranged by size: 388 318 179 148 124 119 34 31 28 20 18 17 10 BsiEI CG_ry'CG Cuts at: 0 692 1434 Size: 692 742 BsiHKAI G_wGCw'C Cuts at: 0 453 920 997 1434 Size: 453 467 77 437 Fragments arranged by size: 467 453 437 77 Bsp1286I G_dGCh'C Cuts at: 0 50 223 453 641 804 920 930 997 Size: 50 173 230 188 163 116 10 67 Cuts at: 997 1240 1434 Size: 243 194 Fragments arranged by size: 243 230 194 188 173 163 116 67 50 10 BspEI T'CCGG_A Cuts at: 0 302 1434 Size: 302 1132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 318 1100 1434 Size: 318 782 334 Fragments arranged by size: 782 334 318 BsrBI CCG'CTC Cuts at: 0 1157 1169 1434 Size: 1157 12 265 Fragments arranged by size: 1157 265 12 BsrDI GCAATG_nn' Cuts at: 0 837 1079 1434 Size: 837 242 355 Fragments arranged by size: 837 355 242 BsrFI r'CCGG_y Cuts at: 0 119 687 1127 1434 Size: 119 568 440 307 Fragments arranged by size: 568 440 307 119 BstAPI GCAn_nnn'nTGC Cuts at: 0 861 1434 Size: 861 573 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 80 495 1434 Size: 57 23 415 939 Fragments arranged by size: 939 415 57 23 BstYI r'GATC_y Cuts at: 0 1205 1434 Size: 1205 229 BtgI C'CryG_G Cuts at: 0 182 1434 Size: 182 1252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 474 996 1434 Size: 474 522 438 Fragments arranged by size: 522 474 438 BtsI GCAGTG_nn' Cuts at: 0 409 1434 Size: 409 1025 EaeI y'GGCC_r Cuts at: 0 59 485 527 689 881 1076 1434 Size: 59 426 42 162 192 195 358 Fragments arranged by size: 426 358 195 192 162 59 42 EagI C'GGCC_G Cuts at: 0 689 1434 Size: 689 745 EarI CTCTTCn'nnn_ Cuts at: 0 916 1434 Size: 916 518 EcoICRI GAG'CTC Cuts at: 0 451 995 1434 Size: 451 544 439 Fragments arranged by size: 544 451 439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 621 645 759 790 1354 1373 1434 Size: 93 528 24 114 31 564 19 61 Fragments arranged by size: 564 528 114 93 61 31 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 285 1434 Size: 285 1149 EcoO109I rG'GnC_Cy Cuts at: 0 171 369 818 1422 1434 Size: 171 198 449 604 12 Fragments arranged by size: 604 449 198 171 12 HaeII r_GCGC'y Cuts at: 0 632 1224 1434 Size: 632 592 210 Fragments arranged by size: 632 592 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 788 820 1434 Size: 788 32 614 Fragments arranged by size: 788 614 32 KasI G'GCGC_C Cuts at: 0 1220 1434 Size: 1220 214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1217 1434 Size: 1217 217 MscI TGG'CCA Cuts at: 0 61 529 883 1078 1434 Size: 61 468 354 195 356 Fragments arranged by size: 468 356 354 195 61 MslI CAynn'nnrTG Cuts at: 0 250 1434 Size: 250 1184 MspA1I CmG'CkG Cuts at: 0 53 80 525 852 858 940 1202 1279 Size: 53 27 445 327 6 82 262 77 Cuts at: 1279 1434 Size: 155 Fragments arranged by size: 445 327 262 155 82 77 53 27 6 NaeI GCC'GGC Cuts at: 0 121 689 1129 1434 Size: 121 568 440 305 Fragments arranged by size: 568 440 305 121 NarI GG'CG_CC Cuts at: 0 1221 1434 Size: 1221 213 NgoMIV G'CCGG_C Cuts at: 0 119 687 1127 1434 Size: 119 568 440 307 Fragments arranged by size: 568 440 307 119 NspI r_CATG'y Cuts at: 0 321 1434 Size: 321 1113 PasI CC'CwG_GG Cuts at: 0 216 1233 1368 1434 Size: 216 1017 135 66 Fragments arranged by size: 1017 216 135 66 PciI A'CATG_T Cuts at: 0 317 1434 Size: 317 1117 PflMI CCAn_nnn'nTGG Cuts at: 0 83 1434 Size: 83 1351 PfoI T'CCnGG_A Cuts at: 0 785 1434 Size: 785 649 PpuMI rG'GwC_Cy Cuts at: 0 171 1422 1434 Size: 171 1251 12 Fragments arranged by size: 1251 171 12 PstI C_TGCA'G Cuts at: 0 289 866 1434 Size: 289 577 568 Fragments arranged by size: 577 568 289 PvuII CAG'CTG Cuts at: 0 53 80 1202 1279 1434 Size: 53 27 1122 77 155 Fragments arranged by size: 1122 155 77 53 27 SacI G_AGCT'C Cuts at: 0 453 997 1434 Size: 453 544 437 Fragments arranged by size: 544 453 437 SanDI GG'GwC_CC Cuts at: 0 171 1434 Size: 171 1263 SexAI A'CCwGG_T Cuts at: 0 506 1434 Size: 506 928 SfcI C'TryA_G Cuts at: 0 285 862 1434 Size: 285 577 572 Fragments arranged by size: 577 572 285 SfiI GGCCn_nnn'nGGCC Cuts at: 0 952 1434 Size: 952 482 SfoI GGC'GCC Cuts at: 0 1222 1434 Size: 1222 212 StuI AGG'CCT Cuts at: 0 1021 1434 Size: 1021 413 StyI C'CwwG_G Cuts at: 0 1016 1349 1400 1434 Size: 1016 333 51 34 Fragments arranged by size: 1016 333 51 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 90 379 1072 1105 1177 1434 Size: 90 289 693 33 72 257 Fragments arranged by size: 693 289 257 90 72 33 TatI w'GTAC_w Cuts at: 0 1067 1434 Size: 1067 367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 80 537 1071 1303 1434 Size: 80 457 534 232 131 Fragments arranged by size: 534 457 232 131 80 Enzymes that do cut: AarI AcuI AflIII AlwNI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SacI SanDI SexAI SfcI SfiI SfoI StuI StyI TaqII TatI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaBI BsgI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI