(Circular) (Six-base) MAPSORT of: pFN21AE9448.seq Check: 8491 from: 1 to: 6139 pFN21AE9448 6139 bp With 182 enzymes: SgfI * March 28, 2012 12:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2323 3105 2323 Size: 782 5357 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2764 4202 5586 5854 1632 Size: 1132 1438 1384 268 1917 Fragments arranged by size: 1917 1438 1384 1132 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2322 2977 5038 6015 1203 Size: 1119 655 2061 977 1327 Fragments arranged by size: 2061 1327 1119 977 655 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 2153 2866 3097 3284 3337 3882 5454 2153 Size: 713 231 187 53 545 1572 2838 Fragments arranged by size: 2838 1572 713 545 231 187 53 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2645 3464 1870 Size: 73 702 819 4545 Fragments arranged by size: 4545 819 702 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 3225 3358 3460 4915 Size: 325 217 627 1217 221 133 102 1455 Cuts at: 4915 618 Size: 1842 Fragments arranged by size: 1842 1455 1217 627 325 221 217 133 102 BanII G_rGCy'C Cuts at: 1391 2055 2458 2809 2935 3002 3245 3458 1391 Size: 664 403 351 126 67 243 213 4072 Fragments arranged by size: 4072 664 403 351 243 213 126 67 BbeI G_GCGC'C Cuts at: 3229 3229 Size: 6139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2374 3407 928 Size: 971 475 1033 3660 Fragments arranged by size: 3660 1033 971 475 BbvCI CC'TCA_GC Cuts at: 3203 3203 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2423 2457 4330 4364 1903 Size: 34 486 34 1873 34 3678 Fragments arranged by size: 3678 1873 486 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 2478 1548 Size: 930 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 4750 136 Size: 107 122 71 631 605 1285 1793 1525 Fragments arranged by size: 1793 1525 1285 631 605 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2228 2646 4200 5356 1790 Size: 438 418 1554 1156 2573 Fragments arranged by size: 2573 1554 1156 438 418 BmgBI CAC'GTC Cuts at: 1318 2980 1318 Size: 1662 4477 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2626 2650 2795 3359 3378 4799 1239 Size: 859 528 24 145 564 19 1421 2579 Fragments arranged by size: 2579 1421 859 564 528 145 24 19 Bpu10I CC'TnA_GC Cuts at: 3203 3203 Size: 6139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 3930 493 Size: 711 1437 1289 2702 Fragments arranged by size: 2702 1437 1289 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2549 3226 3946 4328 Size: 53 83 186 936 1016 677 720 382 Cuts at: 4328 4963 275 Size: 635 1451 Fragments arranged by size: 1451 1016 936 720 677 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2307 4572 4970 5244 5391 5802 2307 Size: 2265 398 274 147 411 2644 Fragments arranged by size: 2644 2265 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2793 2823 1178 Size: 30 165 30 1390 30 4494 Fragments arranged by size: 4494 1390 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2903 1186 Size: 1717 4422 BseYI C'CCAG_C Cuts at: 2036 2054 2071 2081 2260 2578 2966 3090 3118 Size: 18 17 10 179 318 388 124 28 Cuts at: 3118 3152 3300 3419 5342 5838 2036 Size: 34 148 119 1923 496 2337 Fragments arranged by size: 2337 1923 496 388 318 179 148 124 119 34 28 18 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2697 3589 4350 5378 1540 Size: 275 186 696 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 696 275 186 BsiHKAI G_wGCw'C Cuts at: 2458 2925 3002 4200 4285 5356 2458 Size: 467 77 1198 85 1071 3241 Fragments arranged by size: 3241 1198 1071 467 85 77 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2228 2458 2646 2809 2925 2935 Size: 399 265 173 230 188 163 116 10 Cuts at: 2935 3002 3245 3458 4200 4285 5356 1391 Size: 67 243 213 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 265 243 230 213 188 173 163 116 85 67 10 BspEI T'CCGG_A Cuts at: 2307 5802 2307 Size: 3495 2644 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2323 3105 3494 844 Size: 1479 782 389 3489 Fragments arranged by size: 3489 1479 782 389 BsrBI CCG'CTC Cuts at: 1394 1823 3162 3174 4026 1394 Size: 429 1339 12 852 3507 Fragments arranged by size: 3507 1339 852 429 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2842 3084 4634 4808 65 Size: 1183 1594 242 1550 174 1396 Fragments arranged by size: 1594 1550 1396 1183 242 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 2692 3132 4783 4970 1700 Size: 110 314 568 440 1651 187 2869 Fragments arranged by size: 2869 1651 568 440 314 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 2866 794 Size: 2072 4067 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2085 2500 2062 Size: 23 415 5701 Fragments arranged by size: 5701 415 23 BstYI r'GATC_y Cuts at: 1972 3210 3469 3836 4221 4238 5690 5776 5788 Size: 1238 259 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1238 385 367 346 259 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2187 513 Size: 552 1122 4465 Fragments arranged by size: 4465 1122 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2479 3001 487 Size: 683 56 1253 522 3625 Fragments arranged by size: 3625 1253 683 522 56 BtsI GCAGTG_nn' Cuts at: 2414 3655 4449 4469 2414 Size: 1241 794 20 4084 Fragments arranged by size: 4084 1241 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2490 2532 Size: 54 1425 50 275 18 234 426 42 Cuts at: 2532 2694 2886 3081 3586 4475 6104 8 Size: 162 192 195 505 889 1629 43 Fragments arranged by size: 1629 1425 889 505 426 275 234 195 192 162 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2694 3586 1537 Size: 275 882 892 4090 Fragments arranged by size: 4090 892 882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2921 4069 1353 Size: 1568 1148 3423 Fragments arranged by size: 3423 1568 1148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2456 3000 2456 Size: 544 5595 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2626 2650 2764 2795 3359 3378 Size: 393 466 528 24 114 31 564 19 Cuts at: 3378 4202 4799 5586 5854 1239 Size: 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 564 528 466 393 268 114 31 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2290 Size: 6139 EcoO109I rG'GnC_Cy Cuts at: 2176 2374 2823 3427 2176 Size: 198 449 604 4888 Fragments arranged by size: 4888 604 449 198 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2637 3229 5286 1678 Size: 959 592 2057 2531 Fragments arranged by size: 2531 2057 959 592 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2793 2825 4780 4812 4854 Size: 32 135 32 1221 32 1955 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 1955 1221 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 3225 3225 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3222 5253 5437 1305 Size: 1917 2031 184 2007 Fragments arranged by size: 2031 2007 1917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 2534 2888 3083 10 Size: 54 1425 343 234 468 354 195 3066 Fragments arranged by size: 3066 1425 468 354 343 234 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 4097 4456 4615 518 Size: 648 1089 1842 359 159 2042 Fragments arranged by size: 2042 1842 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2085 2530 2857 2863 2945 3207 3284 Size: 452 27 445 327 6 82 262 77 Cuts at: 3284 3555 4232 5380 5625 1606 Size: 271 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 452 445 327 271 262 245 82 77 27 6 NaeI GCC'GGC Cuts at: 2126 2694 3134 2126 Size: 568 440 5131 Fragments arranged by size: 5131 568 440 NarI GG'CG_CC Cuts at: 3226 3226 Size: 6139 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5587 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 2124 2692 3132 2124 Size: 568 440 5131 Fragments arranged by size: 5131 568 440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2326 3497 5042 1132 Size: 97 1097 1171 1545 2229 Fragments arranged by size: 2229 1545 1171 1097 97 PasI CC'CwG_GG Cuts at: 1348 2221 3238 3373 1348 Size: 873 1017 135 4114 Fragments arranged by size: 4114 1017 873 135 PciI A'CATG_T Cuts at: 2322 5038 2322 Size: 2716 3423 PflMI CCAn_nnn'nTGG Cuts at: 1108 2088 1108 Size: 980 5159 PfoI T'CCnGG_A Cuts at: 2790 2790 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 3427 2176 Size: 1251 4888 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 2294 2871 3491 830 Size: 1464 577 620 3478 Fragments arranged by size: 3478 1464 620 577 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2058 2085 3207 3284 2058 Size: 27 1122 77 4913 Fragments arranged by size: 4913 1122 77 27 SacI G_AGCT'C Cuts at: 2458 3002 2458 Size: 544 5595 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SanDI GG'GwC_CC Cuts at: 2176 2176 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SexAI A'CCwGG_T Cuts at: 2511 2511 Size: 6139 SfcI C'TryA_G Cuts at: 826 1045 2290 2867 3487 4622 5303 5494 826 Size: 219 1245 577 620 1135 681 191 1471 Fragments arranged by size: 1471 1245 1135 681 620 577 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2957 2957 Size: 6139 SfoI GGC'GCC Cuts at: 3227 3227 Size: 6139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 3026 1517 Size: 1509 4630 StyI C'CwwG_G Cuts at: 513 1065 3021 3354 3405 3578 513 Size: 552 1956 333 51 173 3074 Fragments arranged by size: 3074 1956 552 333 173 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2095 2384 3077 3110 3182 4166 4183 Size: 1255 515 289 693 33 72 984 17 Cuts at: 4183 4336 4521 4966 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 984 693 515 445 289 185 153 72 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3072 4385 6097 Size: 274 80 33 51 1443 1095 1313 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 1712 1443 1313 1095 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2085 2542 3076 3308 2085 Size: 457 534 232 4916 Fragments arranged by size: 4916 534 457 232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SrfI SwaI