################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MaeIII MamI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 336 CGCG CG^CG AccIII 302 TCCGGA T^CCGGA AvaI 640 CCCGAG C^[CT]CG[GA]G BbeI 1220 GGCGCC GGCGC^C BbvCI 1197 CCTCAGC CCTCAGC(-5/-2) BcgI 429 CGAGTTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 473 TGATCA T^GATCA BcnI 786 CCGGG CC^[GC]GG BglI 946 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 634 TACGTG [CT]AC^GT[GA] BsaWI 302 TCCGGA [AT]^CCGG[AT] BseAI 302 TCCGGA T^CCGGA BseRI 907 CTCCTC GAGGAG(10/8) BsiEI 689 CGGCCG CG[GA][CT]^CG BsoBI 640 CCCGAG C^[CT]CG[GA]G BspEI 302 TCCGGA T^CCGGA BspLU11I 317 ACATGT A^CATGT BsrI 386 CCAGT ACTGG(1/-1) BstAPI 855 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 336 CGCG CG^CG BstYI 1205 GGATCT [GA]^GATC[CT] DdeI 1198 CTCAG C^T[ACGT]AG DsaI 182 CCACGG C^C[GA][CT]GG EagI 689 CGGCCG C^GGCCG EarI 910 CTCTTC CTCTTC(1/4) EclXI 689 CGGCCG C^GGCCG Eco52I 689 CGGCCG C^GGCCG EcoNI 281 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 522 CCCGC CCCGC(4/6) FbaI 473 TGATCA T^GATCA HgaI 544 GCGTC GACGC(5/10) KasI 1220 GGCGCC G^GCGCC Ksp632I 910 CTCTTC CTCTTC(1/4) MflI 1205 GGATCT [GA]^GATC[CT] MroI 302 TCCGGA T^CCGGA MslI 246 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 336 CGCG CG^CG NarI 1220 GGCGCC GG^CGCC NciI 786 CCGGG CC^[GC]GG NspI 317 ACATGT [GA]CATG^[CT] PflMI 77 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SexAI 506 ACCTGGT A^CC[AT]GGT SfiI 945 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1220 GGCGCC GGC^GCC StuI 1019 AGGCCT AGG^CCT Van91I 77 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1205 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 543 GGCGTC G[GA]^CG[CT]C 1220 GGCGCC AfaI 966 GTAC GT^AC 1068 GTAC AflIII 317 ACATGT A^C[GA][CT]GT 972 ACACGT AlwI 1410 GATCC GGATC(4/5) 1205 GGATC BbsI 1395 GAAGAC GAAGAC(2/6) 376 GTCTTC BpuAI 1395 GAAGAC GAAGAC(2/6) 376 GTCTTC BsaHI 543 GGCGTC G[GA]^CG[CT]C 1220 GGCGCC BspMI 309 ACCTGC ACCTGC(4/8) 1091 ACCTGC BsrBI 1155 CCGCTC CCGCTC(-3/-3) 1167 CCGCTC BsrDI 1080 CATTGC GCAATG(2/0) 830 GCAATG CfoI 629 GCGC GCG^C 1221 GCGC HaeII 628 AGCGCC [GA]GCGC^[CT] 1220 GGCGCC HhaI 629 GCGC GCG^C 1221 GCGC Hin1I 543 GGCGTC G[GA]^CG[CT]C 1220 GGCGCC HinP1I 629 GCGC G^CGC 1221 GCGC MaeII 635 ACGT A^CGT 974 ACGT PpuMI 170 GGGTCCC [GA]G^G[AT]CC[CT] 1421 AGGACCC PstI 285 CTGCAG CTGCA^G 862 CTGCAG RsaI 966 GTAC GT^AC 1068 GTAC SacI 449 GAGCTC GAGCT^C 993 GAGCTC SfaNI 252 GATGC GCATC(5/9) 104 GCATC SfcI 285 CTGCAG C^T[GA][CT]AG 862 CTGCAG TspRI 224 CAGTG CA[GC]TG[ACGT][ACGT]^ 403 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaII BalI BanI BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstXI Cac8I Cfr10I Cfr13I DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NaeI NdeII NgoMIV NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI StyI TseI XcmI