GPR25 ORF clone pFN21AE9409

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9409

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Clone info.

Species

human

Clone Name

pFN21AE9409 Sequence (with vector) Restriction Map

Product ID

FHC20758

ORF Sequence

DNA sequence (1083 bp)
Protein sequence (361 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9409

Symbol

GPR25

Description

G protein-coupled receptor 25

Original Clone ID

cp99409

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1083 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 361 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1405.1 1.6e-134 100.0 GPR25 CCDS2931.1 2.8e-29 36.3 GPR15 CCDS3137.1 1e-26 34.0 AGTR1 CCDS82855.1 1.1e-26 34.0 AGTR1 CCDS82854.1 1.1e-26 34.0 AGTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 41 65 PR00237 GPCR IPR000276 74 95 PR00237 GPCR IPR000276 119 141 PR00237 GPCR IPR000276 155 176 PR00237 GPCR IPR000276 203 226 PR00237 GPCR IPR000276 242 266 PR00237 GPCR IPR000276 289 315 PR00237 GPCR HMMPfam IPR000276 56 307 PF00001 GPCR ProfileScan IPR017452 56 307 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 43 PALYLAAFAVGLLGNAFVVWLLA 65 PRIMARY 23 2 77 FVLHLAAADLGFVLTLPLWAAAA 99 SECONDARY 23 3 118 ALAGTRCAGALLLAGMSVDRYLA 140 SECONDARY 23 4 153 PRCALASCCGVWAVALLAGLPSL 175 PRIMARY 23 5 201 GLSLLLLLLTFVLPLVVTLFCYC 223 PRIMARY 23 6 241 NSLRIIFAIESTFVGSWLPFSAL 263 SECONDARY 23 7 266 VFHLARLGALPLPCPLLLALRWG 288 PRIMARY 23 8 293 TCLAFVNSCANPLIYLLLDRSFR 315 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005289 361 602174 100.0 100.0 NP_005281 360 601166 36.3 80.1 NP_000676 359 106165,145500,267430 34.0 81.4 NP_033611 359 106165,145500,267430 34.0 81.4 NP_114438 388 106165,145500,267430 34.0 81.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9409 Download

>KIEE9409  1083 bp
ATGGCCCCCACAGAGCCCTGGAGCCCCAGCCCGGGGTCAGCGCCCTGGGACTACTCGGGG
TTGGACGGCCTGGAGGAGCTGGAGCTGTGTCCGGCCGGGGACCTGCCCTACGGCTACGTC
TACATCCCCGCGCTCTACCTGGCGGCCTTCGCCGTGGGCCTGCTGGGCAACGCCTTTGTG
GTGTGGCTGCTGGCCGGGCGGCGGGGCCCGCGGCGGCTGGTGGATACCTTCGTGCTGCAC
CTGGCGGCAGCTGACCTGGGCTTCGTGCTCACGCTGCCGCTGTGGGCCGCGGCGGCGGCG
CTAGGCGGCCGCTGGCCGTTCGGCGATGGCCTCTGCAAGCTCAGCAGCTTCGCGCTGGCG
GGCACGCGCTGCGCGGGCGCGCTGCTGCTGGCGGGCATGAGCGTGGACCGCTACCTGGCC
GTGGTGAAGCTGCTCGAGGCGAGGCCACTGCGCACCCCGCGCTGCGCGCTGGCCTCGTGC
TGCGGCGTCTGGGCCGTGGCGCTGCTGGCCGGCCTGCCCTCCCTGGTCTACCGGGGGTTG
CAGCCCCTGCCTGGGGGCCAGGACAGCCAGTGCGGCGAGGAGCCCTCCCACGCCTTCCAG
GGCCTCAGCTTGCTGCTGCTGCTGCTGACCTTCGTGCTGCCCCTGGTCGTCACCCTCTTC
TGCTACTGCCGCATCTCGCGCCGCCTGCGACGGCCGCCGCACGTGGGTCGGGCCCGGAGG
AACTCGCTGCGCATCATCTTCGCCATCGAGAGCACGTTTGTGGGCTCCTGGCTGCCCTTC
AGCGCCCTGCGGGCCGTCTTCCACCTGGCGCGTCTGGGGGCGCTGCCGCTGCCGTGCCCC
CTGCTGCTGGCGCTGCGCTGGGGCCTCACCATTGCCACCTGCCTGGCCTTCGTCAACAGC
TGCGCCAACCCGCTCATCTACCTCCTGCTGGACCGCTCATTCCGAGCCCGGGCGCTGGAC
GGGGCCTGCGGGCGCACCGGCCGCCTGGCGCGAAGGATCAGCTCAGCCTCCTCGCTCTCC
AGGGACGACAGTTCCGTGTTCCGTTGCCGGGCCCAGGCCGCGAACACTGCCTCGGCCTCC
TGG

ORF Protein

Cloned ORF protein sequence for pFN21AE9409 Download

>KIEE9409  361 aa
MAPTEPWSPSPGSAPWDYSGLDGLEELELCPAGDLPYGYVYIPALYLAAFAVGLLGNAFV
VWLLAGRRGPRRLVDTFVLHLAAADLGFVLTLPLWAAAAALGGRWPFGDGLCKLSSFALA
GTRCAGALLLAGMSVDRYLAVVKLLEARPLRTPRCALASCCGVWAVALLAGLPSLVYRGL
QPLPGGQDSQCGEEPSHAFQGLSLLLLLLTFVLPLVVTLFCYCRISRRLRRPPHVGRARR
NSLRIIFAIESTFVGSWLPFSALRAVFHLARLGALPLPCPLLLALRWGLTIATCLAFVNS
CANPLIYLLLDRSFRARALDGACGRTGRLARRISSASSLSRDDSSVFRCRAQAANTASAS
W

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9409 Download

>pFN21AE9409  5788 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCCCACAGAGCCCTGGAGCCCCAGCCCGGG
GTCAGCGCCCTGGGACTACTCGGGGTTGGACGGCCTGGAGGAGCTGGAGCTGTGTCCGGC
CGGGGACCTGCCCTACGGCTACGTCTACATCCCCGCGCTCTACCTGGCGGCCTTCGCCGT
GGGCCTGCTGGGCAACGCCTTTGTGGTGTGGCTGCTGGCCGGGCGGCGGGGCCCGCGGCG
GCTGGTGGATACCTTCGTGCTGCACCTGGCGGCAGCTGACCTGGGCTTCGTGCTCACGCT
GCCGCTGTGGGCCGCGGCGGCGGCGCTAGGCGGCCGCTGGCCGTTCGGCGATGGCCTCTG
CAAGCTCAGCAGCTTCGCGCTGGCGGGCACGCGCTGCGCGGGCGCGCTGCTGCTGGCGGG
CATGAGCGTGGACCGCTACCTGGCCGTGGTGAAGCTGCTCGAGGCGAGGCCACTGCGCAC
CCCGCGCTGCGCGCTGGCCTCGTGCTGCGGCGTCTGGGCCGTGGCGCTGCTGGCCGGCCT
GCCCTCCCTGGTCTACCGGGGGTTGCAGCCCCTGCCTGGGGGCCAGGACAGCCAGTGCGG
CGAGGAGCCCTCCCACGCCTTCCAGGGCCTCAGCTTGCTGCTGCTGCTGCTGACCTTCGT
GCTGCCCCTGGTCGTCACCCTCTTCTGCTACTGCCGCATCTCGCGCCGCCTGCGACGGCC
GCCGCACGTGGGTCGGGCCCGGAGGAACTCGCTGCGCATCATCTTCGCCATCGAGAGCAC
GTTTGTGGGCTCCTGGCTGCCCTTCAGCGCCCTGCGGGCCGTCTTCCACCTGGCGCGTCT
GGGGGCGCTGCCGCTGCCGTGCCCCCTGCTGCTGGCGCTGCGCTGGGGCCTCACCATTGC
CACCTGCCTGGCCTTCGTCAACAGCTGCGCCAACCCGCTCATCTACCTCCTGCTGGACCG
CTCATTCCGAGCCCGGGCGCTGGACGGGGCCTGCGGGCGCACCGGCCGCCTGGCGCGAAG
GATCAGCTCAGCCTCCTCGCTCTCCAGGGACGACAGTTCCGTGTTCCGTTGCCGGGCCCA
GGCCGCGAACACTGCCTCGGCCTCCTGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT