(Circular) (Six-base) MAPSORT of: pFN21AE9409.seq Check: 6855 from: 1 to: 5788 pFN21AE9409 5788 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2891 2891 Size: 5788 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 2532 3131 1937 Size: 187 408 599 4594 Fragments arranged by size: 4594 599 408 187 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2767 3851 5235 5503 1632 Size: 1135 1084 1384 268 1917 Fragments arranged by size: 1917 1384 1135 1084 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2762 1166 Size: 1596 4192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2551 3531 5103 2551 Size: 980 1572 3236 Fragments arranged by size: 3236 1572 980 ApaI G_GGCC'C Cuts at: 2213 2719 3058 2213 Size: 506 339 4943 Fragments arranged by size: 4943 506 339 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 3097 3323 3475 5584 1493 Size: 1604 226 152 2109 1697 Fragments arranged by size: 2109 1697 1604 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2059 2438 2952 3113 1870 Size: 73 92 24 379 514 161 4545 Fragments arranged by size: 4545 514 379 161 92 73 24 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2220 2253 2220 Size: 33 5755 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 4564 618 Size: 325 217 627 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2030 2213 2589 2719 2771 2953 3058 Size: 631 8 183 376 130 52 182 105 Cuts at: 3058 3107 1391 Size: 49 4072 Fragments arranged by size: 4072 631 376 183 182 130 105 52 49 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2794 928 Size: 971 895 3922 Fragments arranged by size: 3922 971 895 BbvCI CC'TCA_GC Cuts at: 2609 2609 Size: 5788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2434 2468 2693 2725 2727 2759 3979 Size: 34 497 34 225 32 2 32 1220 Cuts at: 3979 4013 1903 Size: 34 3678 Fragments arranged by size: 3678 1220 497 225 34 34 34 32 32 2 BciVI GTATCCnnnnn_n' Cuts at: 2221 3680 4890 2221 Size: 1459 1210 3119 Fragments arranged by size: 3119 1459 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 2297 2577 Size: 107 122 71 631 605 497 128 280 Cuts at: 2577 2795 4399 136 Size: 218 1604 1525 Fragments arranged by size: 1604 1525 631 605 497 280 218 128 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 2345 3007 3205 2345 Size: 662 198 4928 Fragments arranged by size: 4928 662 198 Bme1580I G_kGCm'C Cuts at: 1790 2213 2369 2719 2843 3058 3849 5005 1790 Size: 423 156 350 124 215 791 1156 2573 Fragments arranged by size: 2573 1156 791 423 350 215 156 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2096 2105 3007 4448 1239 Size: 805 52 9 902 1441 2579 Fragments arranged by size: 2579 1441 902 805 52 9 Bpu10I CC'TnA_GC Cuts at: 2609 2609 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 3579 493 Size: 711 1503 872 2702 Fragments arranged by size: 2702 1503 872 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2490 3595 3977 4612 Size: 53 83 186 936 957 1105 382 635 Cuts at: 4612 275 Size: 1451 Fragments arranged by size: 1451 1105 957 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2597 3004 1186 Size: 907 504 407 3970 Fragments arranged by size: 3970 907 504 407 BseYI C'CCAG_C Cuts at: 2030 2167 2862 4991 5487 2030 Size: 137 695 2129 496 2331 Fragments arranged by size: 2331 2129 695 496 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 1163 Size: 573 489 4726 Fragments arranged by size: 4726 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2100 2314 2699 2986 3238 3999 Size: 275 186 99 214 385 287 252 761 Cuts at: 3999 5027 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 385 287 275 252 214 186 99 BsiHKAI G_wGCw'C Cuts at: 2274 2759 3849 3934 5005 2274 Size: 485 1090 85 1071 3057 Fragments arranged by size: 3057 1090 1071 485 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2030 2213 2274 2369 2589 2719 Size: 399 232 8 183 61 95 220 130 Cuts at: 2719 2759 2771 2843 2953 3058 3107 3849 3934 Size: 40 12 72 110 105 49 742 85 Cuts at: 3934 5005 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 232 220 183 130 110 105 95 85 72 61 49 40 12 8 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2891 3143 844 Size: 1271 776 252 3489 Fragments arranged by size: 3489 1271 776 252 BsrBI CCG'CTC Cuts at: 1394 1823 2917 2940 3675 1394 Size: 429 1094 23 735 3507 Fragments arranged by size: 3507 1094 735 429 23 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 4283 4457 65 Size: 1183 1626 1409 174 1396 Fragments arranged by size: 1626 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2513 2981 4432 4619 1700 Size: 110 703 468 1451 187 2869 Fragments arranged by size: 2869 1451 703 468 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 2382 2469 1925 Size: 457 87 5244 Fragments arranged by size: 5244 457 87 BssSI C'ACGA_G Cuts at: 2479 3848 4860 2479 Size: 1369 1012 3407 Fragments arranged by size: 3407 1369 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2373 2551 794 Size: 1579 178 4031 Fragments arranged by size: 4031 1579 178 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2887 2887 Size: 5788 BstYI r'GATC_y Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783 Size: 1146 367 385 17 1452 86 12 346 Cuts at: 5783 1972 Size: 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 2213 2292 2424 2499 513 Size: 552 939 153 56 79 132 75 3802 Fragments arranged by size: 3802 939 552 153 132 79 75 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2343 487 Size: 683 56 1117 3932 Fragments arranged by size: 3932 1117 683 56 BtsI GCAGTG_nn' Cuts at: 2450 3069 3304 4098 4118 2450 Size: 619 235 794 20 4120 Fragments arranged by size: 4120 794 619 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2196 2311 Size: 54 1425 50 275 18 267 99 115 Cuts at: 2311 2318 2421 2511 2696 2983 3235 4124 5753 Size: 7 103 90 185 287 252 889 1629 Cuts at: 5753 8 Size: 43 Fragments arranged by size: 1629 1425 889 287 275 267 252 185 115 103 99 90 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2097 2311 2696 2983 3235 1537 Size: 275 285 214 385 287 252 4090 Fragments arranged by size: 4090 385 287 285 275 252 214 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3718 1353 Size: 1313 1052 3423 Fragments arranged by size: 3423 1313 1052 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2096 2105 2767 3007 3851 4448 Size: 393 412 52 9 662 240 844 597 Cuts at: 4448 5235 5503 1239 Size: 787 268 1524 Fragments arranged by size: 1524 844 787 662 597 412 393 268 240 52 9 EcoO109I rG'GnC_Cy Cuts at: 2104 2209 2605 2866 2967 2104 Size: 105 396 261 101 4925 Fragments arranged by size: 4925 396 261 105 101 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FseI GG_CCGG'CC Cuts at: 2517 2517 Size: 5788 FspI TGC'GCA Cuts at: 2456 2735 4294 2456 Size: 279 1559 3950 Fragments arranged by size: 3950 1559 279 HaeII r_GCGC'y Cuts at: 1678 2048 2306 2507 2790 2828 2858 2960 4935 Size: 370 258 201 283 38 30 102 1975 Cuts at: 4935 1678 Size: 2531 Fragments arranged by size: 2531 1975 370 283 258 201 102 38 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373 Size: 32 135 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2899 3132 3378 669 Size: 988 281 961 233 246 3079 Fragments arranged by size: 3079 988 961 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 4902 5086 1305 Size: 741 2856 184 2007 Fragments arranged by size: 2856 2007 741 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 2762 3746 4105 4264 518 Size: 648 1239 357 984 359 159 2042 Fragments arranged by size: 2042 1239 984 648 359 357 159 MspA1I CmG'CkG Cuts at: 1606 2215 2255 2284 2294 2316 2833 2904 3204 Size: 609 40 29 10 22 517 71 300 Cuts at: 3204 3881 5029 5274 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 609 517 300 245 71 40 29 22 10 NaeI GCC'GGC Cuts at: 2515 2515 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NgoMIV G'CCGG_C Cuts at: 2513 2513 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 2311 3235 2311 Size: 924 4864 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 2049 1348 Size: 701 5087 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PmlI CAC'GTG Cuts at: 2707 2707 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3059 3091 3857 3889 5370 5402 3059 Size: 32 766 32 1481 32 3445 Fragments arranged by size: 3445 1481 766 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2104 Size: 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2209 2715 3054 2209 Size: 506 339 4943 Fragments arranged by size: 4943 506 339 PspXI vC'TCGA_Gb Cuts at: 2438 2438 Size: 5788 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2255 2904 2255 Size: 649 5139 SacII CC_GC'GG Cuts at: 2216 2295 2216 Size: 79 5709 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 3136 4271 4952 5143 826 Size: 219 2091 1135 681 191 1471 Fragments arranged by size: 2091 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2037 2954 3115 1872 Size: 165 917 161 4545 Fragments arranged by size: 4545 917 165 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 3892 4793 5055 820 Size: 197 926 495 1454 901 262 1553 Fragments arranged by size: 1553 1454 926 901 495 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SrfI GCCC'GGGC Cuts at: 2954 2954 Size: 5788 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 3227 513 Size: 552 939 1223 3074 Fragments arranged by size: 3074 1223 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 5748 5780 2558 Size: 32 3158 32 2566 Fragments arranged by size: 3158 2566 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 2438 1943 Size: 495 5293 XmaI C'CCGG_G Cuts at: 1870 2035 2952 3113 1870 Size: 165 917 161 4545 Fragments arranged by size: 4545 917 165 161 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SwaI XcmI