Restriction map for
pFN21AE9409
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BanI BbeI BclI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI HindIII HinfI HpaI
KasI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 484 GGCGTC G[GA]^CG[CT]C
AlwI 995 GGATC GGATC(4/5)
AlwNI 541 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbrPI 700 CACGTG CAC^GTG
BbsI 796 GTCTTC GAAGAC(2/6)
BbvCI 603 CCTCAGC CCTCAGC(-5/-2)
BciVI 222 GGATAC GTATCC(6/5)
BfaI 301 CTAG C^TAG
BpuAI 796 GTCTTC GAAGAC(2/6)
BsaAI 700 CACGTG [CT]AC^GT[GA]
BsaHI 484 GGCGTC G[GA]^CG[CT]C
BsgI 235 CTGCAC GTGCAG(16/14)
BsrDI 870 CATTGC GCAATG(2/0)
BsrI 567 CCAGT ACTGG(1/-1)
BssSI 474 CTCGTG CACGAG(-5/-1)
BstF5I 123 CATCC GGATG(2/0)
BstXI 875 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 996 GATC ^GATC
EarI 655 CTCTTC CTCTTC(1/4)
FokI 123 CATCC GGATG(9/13)
FseI 507 GGCCGGCC GGCCGG^CC
Hin1I 484 GGCGTC G[GA]^CG[CT]C
HincII 892 GTCAAC GT[CT]^[GA]AC
HindII 892 GTCAAC GT[CT]^[GA]AC
Ksp632I 655 CTCTTC CTCTTC(1/4)
MaeI 301 CTAG C^TAG
MaeIII 649 GTCAC ^GT[ACGT]AC
MboI 996 GATC ^GATC
NaeI 508 GCCGGC GCC^GGC
NdeII 996 GATC ^GATC
NgoMIV 508 GCCGGC G^CCGGC
NlaIII 396 CATG CATG^
NotI 305 GCGGCCGC GC^GGCCGC
PaeR7I 433 CTCGAG C^TCGAG
PmaCI 700 CACGTG CAC^GTG
PmlI 700 CACGTG CAC^GTG
PpuMI 98 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 996 GATC ^GATC
SmlI 433 CTCGAG C^T[CT][GA]AG
Tsp45I 649 GTCAC ^GT[GC]AC
XhoI 433 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 118 GTCTAC GT^[AC][GT]AC
526 GTCTAC
AspHI 265 GTGCTC G[AT]GC[AT]^C
750 GAGCAC
AviII 449 TGCGCA TGC^GCA
728 TGCGCA
BlpI 339 GCTCAGC GC^T[ACGT]AGC
1001 GCTCAGC
Bpu1102I 339 GCTCAGC GC^T[ACGT]AGC
1001 GCTCAGC
BsiHKAI 265 GTGCTC G[AT]GC[AT]^C
750 GAGCAC
BspMI 101 ACCTGC ACCTGC(4/8)
877 ACCTGC
BsrBI 910 CCGCTC CCGCTC(-3/-3)
933 CCGCTC
BsrFI 508 GCCGGC [GA]^CCGG[CT]
976 ACCGGC
BssHII 377 GCGCGC G^CGCGC
464 GCGCGC
BstAPI 362 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
540 GCAGCCCCTGC
CelII 339 GCTCAGC GC^T[ACGT]AGC
1001 GCTCAGC
Cfr10I 508 GCCGGC [GA]^CCGG[CT]
976 ACCGGC
FspI 449 TGCGCA TGC^GCA
728 TGCGCA
HgaI 485 GCGTC GACGC(5/10)
810 GCGTC
KspI 208 CCGCGG CCGC^GG
287 CCGCGG
MslI 396 CATGAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
753 CACGTTTGTG
PvuII 248 CAGCTG CAG^CTG
897 CAGCTG
SacII 208 CCGCGG CCGC^GG
287 CCGCGG
SfaNI 671 GCATC GCATC(5/9)
731 GCATC
SmaI 30 CCCGGG CCC^GGG
947 CCCGGG
TaqI 434 TCGA T^CGA
746 TCGA
TthHB8I 434 TCGA T^CGA
746 TCGA
XmaCI 30 CCCGGG C^CCGGG
947 CCCGGG
XmaI 30 CCCGGG C^CCGGG
947 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
AvaII BanII BbvI BcgI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI TspRI