GPR135 ORF clone pFN21AE9403

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9403

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Clone info.

Species

human

Clone Name

pFN21AE9403 Sequence (with vector) Restriction Map

Product ID

FHC05685

ORF Sequence

DNA sequence (1482 bp)
Protein sequence (494 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9403

Symbol

GPR135

Alias : HUMNPIIY20

Description

G protein-coupled receptor 135

Original Clone ID

cp99403

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1482 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 494 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9738.1 2e-114 100.0 GPR135 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 105 129 PR00237 GPCR IPR000276 138 159 PR00237 GPCR IPR000276 191 213 PR00237 GPCR IPR000276 225 246 PR00237 GPCR IPR000276 278 301 PR00237 GPCR IPR000276 329 353 PR00237 GPCR IPR000276 369 395 PR00237 GPCR HMMPfam IPR000276 120 387 PF00001 GPCR ProfileScan IPR017452 120 387 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 36 AATAAVLSFSTVATAALGNLSDA 58 SECONDARY 23 2 104 AAQALVLLLIFLLSSLGNCAVMG 126 PRIMARY 23 3 141 FILSLSLSDLLTALLCLPAAFLD 163 SECONDARY 23 4 188 RFFSSCFGIVSTLSVALISLDRY 210 PRIMARY 23 5 274 GAAFSVGLVVACYLLPFLLMCFC 296 PRIMARY 23 6 333 VLIMIVFVICCWGPYCFLVLLAA 355 PRIMARY 23 7 364 APSLLSVVAVWLTWANGAINPVI 386 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016877085 494 607970 100.0 100.0 XP_016877086 494 607970 100.0 100.0 XP_016877087 494 607970 100.0 100.0 NP_072093 494 607970 100.0 100.0 XP_016877088 494 607970 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9403 Download

>KIEE9403  1482 bp
ATGGAGGAGCCGCAGCCGCCCCGCCCACCAGCGAGCATGGCCTTACTGGGCAGCCAGCAC
TCCGGCGCCCCCTCCGCGGCCGGCCCACCTGGCGGGACTTCCTCCGCGGCCACGGCGGCC
GTGCTCTCCTTCAGCACCGTGGCGACCGCGGCGCTGGGGAACCTGAGCGACGCAAGCGGA
GGCGGCACAGCTGCCGCTCCCGGTGGCGGCGGCCTTGGCGGGTCCGGGGCAGCGCGGGAG
GCGGGGGCGGCGGTGAGGCGGCCGCTAGGCCCGGAGGCGGCGCCGCTGCTGTCGCACGGA
GCTGCAGTGGCGGCCCAGGCGCTCGTCCTCCTGCTCATCTTCCTGCTGTCTAGCCTTGGC
AACTGCGCGGTGATGGGGGTGATTGTGAAGCACCGGCAGCTCCGCACCGTCACCAACGCC
TTCATCCTGTCGCTGTCCCTATCGGATCTGCTCACGGCGCTGCTCTGCCTGCCCGCCGCC
TTCCTGGACCTCTTCACTCCGCCCGGGGGTTCGGCGCCTGCCGCCGCCGCGGGGCCCTGG
CGCGGCTTCTGCGCCGCCAGCCGCTTCTTCAGCTCGTGCTTCGGCATCGTGTCCACGCTC
AGCGTGGCGCTCATCTCGTTGGACCGTTACTGCGCTATCGTGCGGCCGCCGCGGGAGAAG
ATCGGCCGCCGCCGCGCGCTGCAGCTGCTGGCGGGCGCCTGGCTGACGGCCCTGGGCTTC
TCCTTGCCCTGGGAGCTGCTCGGGGCGCCCCGGGAACTCGCGGCGGCGCAGAGCTTCCAC
GGCTGCCTCTACCGGACCTCCCCGGACCCCGCGCAGCTGGGCGCGGCCTTCAGCGTGGGG
CTGGTGGTGGCCTGCTACCTGCTGCCCTTCCTGCTCATGTGCTTCTGCCACTACCACATC
TGCAAGACGGTGCGCCTGTCGGACGTGCGCGTGCGGCCGGTGAACACCTACGCGCGCGTG
CTGCGCTTCTTCAGCGAGGTGCGCACGGCCACCACCGTCCTCATCATGATCGTCTTCGTC
ATCTGCTGCTGGGGGCCCTACTGCTTCCTGGTGCTGCTGGCCGCCGCCCGGCAGGCCCAG
ACCATGCAGGCCCCCTCGCTCCTCAGCGTGGTGGCCGTCTGGCTGACCTGGGCCAATGGG
GCCATCAACCCTGTCATCTACGCCATCCGCAATCCCAACATTTCGATGCTCCTAGGGCGC
AACCGCGAGGAGGGCTACCGGACTAGGAATGTGGACGCTTTCCTGCCCAGCCAGGGCCCG
GGTCTGCAAGCCAGAAGCCGCAGTCGCCTTCGAAACCGCTATGCCAACCGGCTGGGGGCC
TGCAACAGGATGTCCTCTTCCAACCCGGCCAGCGGAGTGGCAGGGGACGTGGCCATGTGG
GCCCGCAAAAATCCAGTTGTACTTTTCTGCCGAGAGGGACCACCAGAGCCGGTGACGGCA
GTGACCAAACAGCCTAAATCCGAAGCTGGGGATACCAGCCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE9403 Download

>KIEE9403  494 aa
MEEPQPPRPPASMALLGSQHSGAPSAAGPPGGTSSAATAAVLSFSTVATAALGNLSDASG
GGTAAAPGGGGLGGSGAAREAGAAVRRPLGPEAAPLLSHGAAVAAQALVLLLIFLLSSLG
NCAVMGVIVKHRQLRTVTNAFILSLSLSDLLTALLCLPAAFLDLFTPPGGSAPAAAAGPW
RGFCAASRFFSSCFGIVSTLSVALISLDRYCAIVRPPREKIGRRRALQLLAGAWLTALGF
SLPWELLGAPRELAAAQSFHGCLYRTSPDPAQLGAAFSVGLVVACYLLPFLLMCFCHYHI
CKTVRLSDVRVRPVNTYARVLRFFSEVRTATTVLIMIVFVICCWGPYCFLVLLAAARQAQ
TMQAPSLLSVVAVWLTWANGAINPVIYAIRNPNISMLLGRNREEGYRTRNVDAFLPSQGP
GLQARSRSRLRNRYANRLGACNRMSSSNPASGVAGDVAMWARKNPVVLFCREGPPEPVTA
VTKQPKSEAGDTSL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9403 Download

>pFN21AE9403  6187 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGAGCCGCAGCCGCCCCGCCCACCAGCGAG
CATGGCCTTACTGGGCAGCCAGCACTCCGGCGCCCCCTCCGCGGCCGGCCCACCTGGCGG
GACTTCCTCCGCGGCCACGGCGGCCGTGCTCTCCTTCAGCACCGTGGCGACCGCGGCGCT
GGGGAACCTGAGCGACGCAAGCGGAGGCGGCACAGCTGCCGCTCCCGGTGGCGGCGGCCT
TGGCGGGTCCGGGGCAGCGCGGGAGGCGGGGGCGGCGGTGAGGCGGCCGCTAGGCCCGGA
GGCGGCGCCGCTGCTGTCGCACGGAGCTGCAGTGGCGGCCCAGGCGCTCGTCCTCCTGCT
CATCTTCCTGCTGTCTAGCCTTGGCAACTGCGCGGTGATGGGGGTGATTGTGAAGCACCG
GCAGCTCCGCACCGTCACCAACGCCTTCATCCTGTCGCTGTCCCTATCGGATCTGCTCAC
GGCGCTGCTCTGCCTGCCCGCCGCCTTCCTGGACCTCTTCACTCCGCCCGGGGGTTCGGC
GCCTGCCGCCGCCGCGGGGCCCTGGCGCGGCTTCTGCGCCGCCAGCCGCTTCTTCAGCTC
GTGCTTCGGCATCGTGTCCACGCTCAGCGTGGCGCTCATCTCGTTGGACCGTTACTGCGC
TATCGTGCGGCCGCCGCGGGAGAAGATCGGCCGCCGCCGCGCGCTGCAGCTGCTGGCGGG
CGCCTGGCTGACGGCCCTGGGCTTCTCCTTGCCCTGGGAGCTGCTCGGGGCGCCCCGGGA
ACTCGCGGCGGCGCAGAGCTTCCACGGCTGCCTCTACCGGACCTCCCCGGACCCCGCGCA
GCTGGGCGCGGCCTTCAGCGTGGGGCTGGTGGTGGCCTGCTACCTGCTGCCCTTCCTGCT
CATGTGCTTCTGCCACTACCACATCTGCAAGACGGTGCGCCTGTCGGACGTGCGCGTGCG
GCCGGTGAACACCTACGCGCGCGTGCTGCGCTTCTTCAGCGAGGTGCGCACGGCCACCAC
CGTCCTCATCATGATCGTCTTCGTCATCTGCTGCTGGGGGCCCTACTGCTTCCTGGTGCT
GCTGGCCGCCGCCCGGCAGGCCCAGACCATGCAGGCCCCCTCGCTCCTCAGCGTGGTGGC
CGTCTGGCTGACCTGGGCCAATGGGGCCATCAACCCTGTCATCTACGCCATCCGCAATCC
CAACATTTCGATGCTCCTAGGGCGCAACCGCGAGGAGGGCTACCGGACTAGGAATGTGGA
CGCTTTCCTGCCCAGCCAGGGCCCGGGTCTGCAAGCCAGAAGCCGCAGTCGCCTTCGAAA
CCGCTATGCCAACCGGCTGGGGGCCTGCAACAGGATGTCCTCTTCCAACCCGGCCAGCGG
AGTGGCAGGGGACGTGGCCATGTGGGCCCGCAAAAATCCAGTTGTACTTTTCTGCCGAGA
GGGACCACCAGAGCCGGTGACGGCAGTGACCAAACAGCCTAAATCCGAAGCTGGGGATAC
CAGCCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT