################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbrPI BcgI BclI BfrI BglII BpmI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HinfI HpaI KpnI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 1399 GTAC GT^AC AlwI 444 GGATC GGATC(4/5) AlwNI 682 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 121 GTGCTC G[AT]GC[AT]^C AviII 980 TGCGCA TGC^GCA AvrII 1191 CCTAGG C^CTAGG BalI 1370 TGGCCA TGG^CCA BbsI 1012 GTCTTC GAAGAC(2/6) BbvCI 1101 CCTCAGC CCTCAGC(-5/-2) BciVI 1470 GGATAC GTATCC(6/5) BlnI 1191 CCTAGG C^CTAGG BlpI 597 GCTCAGC GC^T[ACGT]AGC Bpu1102I 597 GCTCAGC GC^T[ACGT]AGC BpuAI 1012 GTCTTC GAAGAC(2/6) BsiHKAI 121 GTGCTC G[AT]GC[AT]^C BspHI 1004 TCATGA T^CATGA BspMI 857 ACCTGC ACCTGC(4/8) BsrBI 194 CCGCTC CCGCTC(-3/-3) BssSI 573 CTCGTG CACGAG(-5/-1) BstBI 1289 TTCGAA TT^CGAA BstYI 444 GGATCT [GA]^GATC[CT] CelII 597 GCTCAGC GC^T[ACGT]AGC EcoNI 1319 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 78 GGCCGGCC GGCCGG^CC FspI 980 TGCGCA TGC^GCA MflI 444 GGATCT [GA]^GATC[CT] MluNI 1370 TGGCCA TGG^CCA MscI 1370 TGGCCA TGG^CCA MslI 1179 CATTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 79 GCCGGC GCC^GGC NgoMIV 79 GCCGGC G^CCGGC NspV 1289 TTCGAA TT^CGAA RcaI 1004 TCATGA T^CATGA RsaI 1399 GTAC GT^AC SfuI 1289 TTCGAA TT^CGAA XcmI 593 CCACGCTCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 444 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BsaWI 791 ACCGGA [AT]^CCGG[AT] 1217 ACCGGA BsrI 45 ACTGG ACTGG(1/-1) 1393 CCAGT BssHII 674 GCGCGC G^CGCGC 952 GCGCGC BstXI 1304 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1349 CCAGCGGAGTGG EarI 490 CTCTTC CTCTTC(1/4) 1335 CTCTTC HgaI 169 GACGC GACGC(5/10) 1234 GACGC Ksp632I 490 CTCTTC CTCTTC(1/4) 1335 CTCTTC MaeII 923 ACGT A^CGT 1367 ACGT NotI 258 GCGGCCGC GC^GGCCGC 642 GCGGCCGC PstI 302 CTGCAG CTGCA^G 679 CTGCAG SfaNI 1185 GATGC GCATC(5/9) 584 GCATC SfcI 302 CTGCAG C^T[GA][CT]AG 679 CTGCAG TaqI 1183 TCGA T^CGA 1290 TCGA TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^ 1439 CAGTG TthHB8I 1183 TCGA T^CGA 1290 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PvuII SacII Sau3AI Sau96I ScrFI SfiI SfoI SmaI StyI TseI Tsp45I XmaCI XmaI