(Circular) (Six-base) MAPSORT of: pFN21AE9403.seq Check: 4678 from: 1 to: 6187 pFN21AE9403 6187 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 2557 2818 2959 4250 5634 5902 1632 Size: 487 438 261 141 1291 1384 268 1917 Fragments arranged by size: 1917 1384 1291 487 438 268 261 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2322 2930 1184 Size: 1138 608 4441 Fragments arranged by size: 4441 1138 608 AlwNI CAG_nnn'CTG Cuts at: 2692 3930 5502 2692 Size: 1238 1572 3377 Fragments arranged by size: 3377 1572 1238 ApaI G_GGCC'C Cuts at: 2541 3042 3263 3388 2541 Size: 501 221 125 5340 Fragments arranged by size: 5340 501 221 125 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 3496 3722 3874 5983 1493 Size: 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2507 2744 2754 3262 3512 1870 Size: 73 564 237 10 508 250 4545 Fragments arranged by size: 4545 564 508 250 237 73 10 AvrII C'CTAG_G Cuts at: 3196 3196 Size: 6187 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2284 2518 2699 2749 Size: 325 217 627 282 215 234 181 50 Cuts at: 2749 3508 4963 618 Size: 759 1455 1842 Fragments arranged by size: 1842 1455 759 627 325 282 234 217 215 181 50 BanII G_rGCy'C Cuts at: 1391 2541 3042 3263 3388 3506 1391 Size: 1150 501 221 125 118 4072 Fragments arranged by size: 4072 1150 501 221 125 118 BbeI G_GCGC'C Cuts at: 2073 2288 2522 2703 2753 2073 Size: 215 234 181 50 5507 Fragments arranged by size: 5507 234 215 181 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3010 928 Size: 971 1111 4105 Fragments arranged by size: 4105 1111 971 BbvCI CC'TCA_GC Cuts at: 3107 3107 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3469 4079 5289 3469 Size: 610 1210 4367 Fragments arranged by size: 4367 1210 610 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2094 2120 3077 Size: 107 122 71 631 605 422 26 957 Cuts at: 3077 3143 3383 4798 136 Size: 66 240 1415 1525 Fragments arranged by size: 1525 1415 957 631 605 422 240 122 107 71 66 26 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 2603 3604 2603 Size: 1001 5186 Bme1580I G_kGCm'C Cuts at: 1790 2541 3042 3263 3388 4248 5404 1790 Size: 751 501 221 125 860 1156 2573 Fragments arranged by size: 2573 1156 860 751 501 221 125 BmgBI CAC'GTC Cuts at: 1318 2929 3373 1318 Size: 1611 444 4132 Fragments arranged by size: 4132 1611 444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 3918 4876 Size: 416 53 83 399 24 643 1858 958 Cuts at: 4876 442 Size: 1753 Fragments arranged by size: 1858 1753 958 643 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4847 1239 Size: 3608 2579 Bpu10I CC'TnA_GC Cuts at: 2168 3107 2168 Size: 939 5248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2285 2519 2700 Size: 53 83 186 936 537 215 234 181 Cuts at: 2700 2750 3994 4376 5011 275 Size: 50 1244 382 635 1451 Fragments arranged by size: 1451 1244 936 635 537 382 234 215 186 181 83 53 50 BsaWI w'CCGG_w Cuts at: 2796 3222 4620 5018 5292 5439 5850 2796 Size: 426 1398 398 274 147 411 3133 Fragments arranged by size: 3133 1398 426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2295 2325 2786 2816 1178 Size: 30 165 30 892 30 461 30 4549 Fragments arranged by size: 4549 892 461 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 3095 3227 1186 Size: 838 1071 132 4146 Fragments arranged by size: 4146 1071 838 132 BseYI C'CCAG_C Cuts at: 2158 2821 3033 3251 3316 3470 5390 5886 2158 Size: 663 212 218 65 154 1920 496 2459 Fragments arranged by size: 2459 1920 663 496 218 212 154 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5614 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2085 2124 2151 2267 2651 2671 Size: 275 186 84 39 27 116 384 20 Cuts at: 2671 2942 3637 4398 5426 1540 Size: 271 695 761 1028 2301 Fragments arranged by size: 2301 1028 761 695 384 275 271 186 116 84 39 27 20 BsiHKAI G_wGCw'C Cuts at: 2130 4248 4333 5404 2130 Size: 2118 85 1071 2913 Fragments arranged by size: 2913 2118 1071 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2541 3042 3263 3388 3506 4248 Size: 399 340 411 501 221 125 118 742 Cuts at: 4248 4333 5404 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 501 411 399 340 221 125 118 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 3009 4076 5806 3009 Size: 1067 1730 3390 Fragments arranged by size: 3390 1730 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2871 3542 844 Size: 2027 671 3489 Fragments arranged by size: 3489 2027 671 BsrBI CCG'CTC Cuts at: 1394 1823 2201 4074 1394 Size: 429 378 1873 3507 Fragments arranged by size: 3507 1873 429 378 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2397 2941 3312 3433 4831 5018 Size: 110 274 313 544 371 121 1398 187 Cuts at: 5018 1700 Size: 2869 Fragments arranged by size: 2869 1398 544 371 313 274 187 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 2679 2957 1925 Size: 754 278 5155 Fragments arranged by size: 5155 754 278 BssSI C'ACGA_G Cuts at: 2578 4247 5259 2578 Size: 1669 1012 3506 Fragments arranged by size: 3506 1669 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 3295 4991 3295 Size: 1696 4491 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 3316 3361 3316 Size: 45 6142 BstYI r'GATC_y Cuts at: 1972 2449 3517 3884 4269 4286 5738 5824 5836 Size: 477 1068 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1068 477 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2109 2115 2142 2151 2532 Size: 552 939 75 30 6 27 9 381 Cuts at: 2532 2654 2782 513 Size: 122 128 3918 Fragments arranged by size: 3918 939 552 381 128 122 75 30 27 9 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 2316 3450 3703 4497 4517 2316 Size: 1134 253 794 20 3986 Fragments arranged by size: 3986 1134 794 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2112 2121 Size: 54 1425 50 275 18 252 30 9 Cuts at: 2121 2264 2648 2668 2939 2991 3063 3117 3351 Size: 143 384 20 271 52 72 54 234 Cuts at: 3351 3375 3634 4523 6152 8 Size: 24 259 889 1629 43 Fragments arranged by size: 1629 1425 889 384 275 271 259 252 234 143 72 54 54 52 50 43 30 24 20 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2082 2121 2264 2648 2668 2939 3634 Size: 275 270 39 143 384 20 271 695 Cuts at: 3634 1537 Size: 4090 Fragments arranged by size: 4090 695 384 275 271 270 143 39 20 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 3346 4117 1353 Size: 1148 845 771 3423 Fragments arranged by size: 3423 1148 845 771 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2493 4774 5148 5294 387 Size: 1064 138 904 2281 374 146 1280 Fragments arranged by size: 2281 1280 1064 904 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2557 2818 2959 4250 4847 5634 Size: 393 487 438 261 141 1291 597 787 Cuts at: 5634 5902 1239 Size: 268 1524 Fragments arranged by size: 1524 1291 787 597 487 438 393 268 261 141 EcoNI CCTnn'n_nnAGG Cuts at: 3328 3328 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 2537 2538 3038 3039 3094 3259 3321 2537 Size: 1 500 1 55 165 62 5403 Fragments arranged by size: 5403 500 165 62 55 1 1 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FseI GG_CCGG'CC Cuts at: 2088 2088 Size: 6187 FspI TGC'GCA Cuts at: 2987 4693 2987 Size: 1706 4481 FspAI rTGC'GCAy Cuts at: 2987 2987 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 2073 2159 2288 2327 2465 2522 2615 2703 Size: 395 86 129 39 138 57 93 88 Cuts at: 2703 2753 5334 1678 Size: 50 2581 2531 Fragments arranged by size: 2581 2531 395 138 129 93 88 86 57 50 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373 Size: 32 135 32 3256 32 42 32 2626 Fragments arranged by size: 3256 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KasI G'GCGC_C Cuts at: 2069 2284 2518 2699 2749 2069 Size: 215 234 181 50 5507 Fragments arranged by size: 5507 234 215 181 50 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2604 2904 3369 5301 5485 1305 Size: 1299 300 465 1932 184 2007 Fragments arranged by size: 2007 1932 1299 465 300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3377 10 Size: 54 1425 343 1545 2820 Fragments arranged by size: 2820 1545 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3188 4145 4504 4663 518 Size: 648 2022 957 359 159 2042 Fragments arranged by size: 2042 2022 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 2111 2153 2195 2290 2534 2656 2689 Size: 475 30 42 42 95 244 122 33 Cuts at: 2689 2821 3357 3603 4280 5428 5673 1606 Size: 132 536 246 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 475 246 245 244 132 122 95 42 42 33 30 NaeI GCC'GGC Cuts at: 2086 2086 Size: 6187 NarI GG'CG_CC Cuts at: 2070 2285 2519 2700 2750 2070 Size: 215 234 181 50 5507 Fragments arranged by size: 5507 234 215 181 50 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NgoMIV G'CCGG_C Cuts at: 2084 2084 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 2264 2648 3634 2264 Size: 384 986 4817 Fragments arranged by size: 4817 986 384 NspI r_CATG'y Cuts at: 1132 1229 3545 5090 1132 Size: 97 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2716 2733 1348 Size: 1368 17 4802 Fragments arranged by size: 4802 1368 17 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2487 2487 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PspOMI G'GGCC_C Cuts at: 2537 3038 3259 3384 2537 Size: 501 221 125 5340 Fragments arranged by size: 5340 501 221 125 PstI C_TGCA'G Cuts at: 830 2311 2688 3539 830 Size: 1481 377 851 3478 Fragments arranged by size: 3478 1481 851 377 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2195 2689 2821 2195 Size: 494 132 5561 Fragments arranged by size: 5561 494 132 SacII CC_GC'GG Cuts at: 2082 2112 2154 2535 2657 2082 Size: 30 42 381 122 5612 Fragments arranged by size: 5612 381 122 42 30 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2307 2684 3535 4670 5351 5542 826 Size: 219 1262 377 851 1135 681 191 1471 Fragments arranged by size: 1471 1262 1135 851 681 377 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2120 3143 3383 2120 Size: 1023 240 4924 Fragments arranged by size: 4924 1023 240 SfoI GGC'GCC Cuts at: 2071 2286 2520 2701 2751 2071 Size: 215 234 181 50 5507 Fragments arranged by size: 5507 234 215 181 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 2509 2756 3264 3514 1872 Size: 637 247 508 250 4545 Fragments arranged by size: 4545 637 508 250 247 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 2218 2359 3196 3626 513 Size: 552 939 214 141 837 430 3074 Fragments arranged by size: 3074 939 837 552 430 214 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4231 4384 4569 5014 325 Size: 1255 2634 17 153 185 445 1498 Fragments arranged by size: 2634 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3403 4433 6145 Size: 274 80 33 51 1443 1426 1030 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 1712 1443 1426 1030 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2295 2327 6147 6179 2295 Size: 32 3820 32 2303 Fragments arranged by size: 3820 2303 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 2507 2754 3262 3512 1870 Size: 637 247 508 250 4545 Fragments arranged by size: 4545 637 508 250 247 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI