RSPH4A ORF clone pFN21AE6773

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6773

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Clone info.

Species

human

Clone Name

pFN21AE6773 Sequence (with vector) Restriction Map

Product ID

FHC13228

ORF Sequence

DNA sequence (1800 bp)
Protein sequence (600 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6773

Symbol

RSPH4A

Alias : CILD11, RSHL3, RSPH6B, dJ412I7.1

Description

radial spoke head 4 homolog A (Chlamydomonas), transcript variant 2

Original Clone ID

oc96773

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1800 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 600 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55051.1 8.8e-119 100.0 RSPH4A CCDS34521.1 9.3e-109 100.0 RSPH4A CCDS12675.1 6.7e-33 48.3 RSPH6A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam NULL 209 554 PF04712 NULL
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001155136 600 612647,612649 100.0 100.0 XP_016866315 600 612647,612649 100.0 100.0 NP_001010892 716 612647,612649 100.0 92.3 XP_011525653 647 607548 48.3 85.5 NP_110412 717 607548 48.3 85.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6773 Download

>KIEE6773  1800 bp
ATGGAGGACTCAACCTCCCCGAAGCAAGAAAAAGAAAACCAAGAAGAACTAGGGGAAACA
AGGCGGCCATGGGAAGGAAAGACAGCAGCTTCTCCCCAATATTCTGAGCCTGAGTCGTCT
GAGCCCTTGGAGGCGAAGCAGGGGCCAGAAACTGGACGCCAGTCCCGAAGCAGCCGTCCT
TGGAGCCCGCAGTCTAGAGCCAAGACGCCTCTGGGTGGCCCCGCGGGACCAGAAACATCA
TCACCTGCTCCTGTCTCTCCGCGGGAGCCCTCTTCCTCTCCTTCTCCCCTGGCTCCGGCC
AGACAAGACCTCGCGGCACCACCTCAGTCGGACAGGACCACGAGTGTGATTCCTGAAGCT
GGGACACCTTATCCTGATCCTTTGGAACAATCATCTGATAAAAGAGAATCAACTCCTCAT
CACACAAGCCAGTCAGAAGGAAACACCTTTCAACAGTCTCAGCAACCCAAACCCCACCTG
TGTGGACGAAGGGACGTGAGCTATAACAACGCTAAACAGAAAGAGCTGAGATTTGACGTT
TTTCAGGAGGAAGACTCAAACAGTGACTATGATTTACAGCAGCCGGCGCCTGGGGGCTCT
GAAGTGGCCCCCAGCATGCTTGAGATCACCATTCAGAATGCTAAGGCTTACCTGCTGAAG
ACTAGCAGCAATTCGGGCTTTAATCTATATGATCATCTTTCTAATATGTTGACCAAGATA
TTAAATGAGCGTCCTGAAAATGCTGTTGACATCTTTGAAAATATTAGCCAAGATGTGAAG
ATGGCACATTTTAGTAAAAAATTTGATGCACTACAAAATGAGAATGAGTTGCTTCCAACA
TATGAAATAGCAGAAAAGCAAAAGGCTCTTTTTCTCCAGGGACATTTGGAAGGAGTTGAC
CAAGAATTGGAAGATGAAATAGCAGAAAACGCTCTTCCAAATGTAATGGAGTCAGCTTTT
TATTTTGAACAAGCTGGAGTTGGTTTGGGCACAGATGAGACATACCGCATATTTCTTGCC
CTCAAGCAGCTTACTGATACCCACCCAATCCAAAGATGCCGCTTCTGGGGAAAGATCTTG
GGTCTGGAAATGAATTATATTGTAGCTGAAGTGGAATTTCGTGAGGGGGAAGATGAAGAG
GAAGTGGAAGAGGAAGATGTAGCTGAAGAGAGGGACAATGGAGAAAGTGAAGCTCATGAA
GATGAGGAAGATGAATTACCAAAGTCCTTTTACAAGGCCCCACAGGCTATACCAAAAGAA
GAAAGTAGAACAGGTGCCAACAAATATGTCTATTTTGTTTGCAATGAACCAGGAAGACCA
TGGGTGAAGTTACCACCAGTTATACCTGCACAAATTGTTATTGCAAGAAAAATCAAGAAA
TTTTTCACTGGGCGATTGGATGCTCCCATCATAAGCTACCCACCTTTCCCAGGAAATGAG
AGTAATTATTTACGAGCACAAATTGCCCGAATTTCAGCAGGAACCCACGTCAGTCCTCTA
GGATTTTATCAGTTTGGTGAAGAGGAAGGAGAGGAGGAGGAAGAGGCAGAAGGTGGGCGA
AATAGCTTTGAGGAAAACCCTGATTTTGAAGGCATCCAAGTGATTGATCTAGTAGAATCC
CTATCCAATTGGGTTCATCATGTACAGCATATTCTCTCTCAGAGATTCAGAATATACCCC
CCTGGACAACACGGTTATCCTCAAATCTCATTCCACAATATGCTATTGCAGTCCTTCAAT
CCAACCTTTGGCCTGGAGCATATGCCTTCTCCAATGGCAAAAAGTTTGAAAATTTCTACA

ORF Protein

Cloned ORF protein sequence for pFN21AE6773 Download

>KIEE6773  600 aa
MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRP
WSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEA
GTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDV
FQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKI
LNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVD
QELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKIL
GLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKE
ESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNE
SNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVES
LSNWVHHVQHILSQRFRIYPPGQHGYPQISFHNMLLQSFNPTFGLEHMPSPMAKSLKIST

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6773 Download

>pFN21AE6773  6505 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGACTCAACCTCCCCGAAGCAAGAAAAAGA
AAACCAAGAAGAACTAGGGGAAACAAGGCGGCCATGGGAAGGAAAGACAGCAGCTTCTCC
CCAATATTCTGAGCCTGAGTCGTCTGAGCCCTTGGAGGCGAAGCAGGGGCCAGAAACTGG
ACGCCAGTCCCGAAGCAGCCGTCCTTGGAGCCCGCAGTCTAGAGCCAAGACGCCTCTGGG
TGGCCCCGCGGGACCAGAAACATCATCACCTGCTCCTGTCTCTCCGCGGGAGCCCTCTTC
CTCTCCTTCTCCCCTGGCTCCGGCCAGACAAGACCTCGCGGCACCACCTCAGTCGGACAG
GACCACGAGTGTGATTCCTGAAGCTGGGACACCTTATCCTGATCCTTTGGAACAATCATC
TGATAAAAGAGAATCAACTCCTCATCACACAAGCCAGTCAGAAGGAAACACCTTTCAACA
GTCTCAGCAACCCAAACCCCACCTGTGTGGACGAAGGGACGTGAGCTATAACAACGCTAA
ACAGAAAGAGCTGAGATTTGACGTTTTTCAGGAGGAAGACTCAAACAGTGACTATGATTT
ACAGCAGCCGGCGCCTGGGGGCTCTGAAGTGGCCCCCAGCATGCTTGAGATCACCATTCA
GAATGCTAAGGCTTACCTGCTGAAGACTAGCAGCAATTCGGGCTTTAATCTATATGATCA
TCTTTCTAATATGTTGACCAAGATATTAAATGAGCGTCCTGAAAATGCTGTTGACATCTT
TGAAAATATTAGCCAAGATGTGAAGATGGCACATTTTAGTAAAAAATTTGATGCACTACA
AAATGAGAATGAGTTGCTTCCAACATATGAAATAGCAGAAAAGCAAAAGGCTCTTTTTCT
CCAGGGACATTTGGAAGGAGTTGACCAAGAATTGGAAGATGAAATAGCAGAAAACGCTCT
TCCAAATGTAATGGAGTCAGCTTTTTATTTTGAACAAGCTGGAGTTGGTTTGGGCACAGA
TGAGACATACCGCATATTTCTTGCCCTCAAGCAGCTTACTGATACCCACCCAATCCAAAG
ATGCCGCTTCTGGGGAAAGATCTTGGGTCTGGAAATGAATTATATTGTAGCTGAAGTGGA
ATTTCGTGAGGGGGAAGATGAAGAGGAAGTGGAAGAGGAAGATGTAGCTGAAGAGAGGGA
CAATGGAGAAAGTGAAGCTCATGAAGATGAGGAAGATGAATTACCAAAGTCCTTTTACAA
GGCCCCACAGGCTATACCAAAAGAAGAAAGTAGAACAGGTGCCAACAAATATGTCTATTT
TGTTTGCAATGAACCAGGAAGACCATGGGTGAAGTTACCACCAGTTATACCTGCACAAAT
TGTTATTGCAAGAAAAATCAAGAAATTTTTCACTGGGCGATTGGATGCTCCCATCATAAG
CTACCCACCTTTCCCAGGAAATGAGAGTAATTATTTACGAGCACAAATTGCCCGAATTTC
AGCAGGAACCCACGTCAGTCCTCTAGGATTTTATCAGTTTGGTGAAGAGGAAGGAGAGGA
GGAGGAAGAGGCAGAAGGTGGGCGAAATAGCTTTGAGGAAAACCCTGATTTTGAAGGCAT
CCAAGTGATTGATCTAGTAGAATCCCTATCCAATTGGGTTCATCATGTACAGCATATTCT
CTCTCAGAGATTCAGAATATACCCCCCTGGACAACACGGTTATCCTCAAATCTCATTCCA
CAATATGCTATTGCAGTCCTTCAATCCAACCTTTGGCCTGGAGCATATGCCTTCTCCAAT
GGCAAAAAGTTTGAAAATTTCTACAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT