Restriction map for
pFN21AE6773
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
MamI MluI MluNI MroI MscI
NciI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 1642 GTAC GT^AC
AlwI 376 GATCC GGATC(4/5)
AlwNI 146 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1454 GAGCAC G[AT]GC[AT]^C
BbeI 585 GGCGCC GGCGC^C
BclI 690 TGATCA T^GATCA
BglI 1237 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1073 AGATCT A^GATCT
BsgI 1346 CTGCAC GTGCAG(16/14)
BsiHKAI 1454 GAGCAC G[AT]GC[AT]^C
BsmI 636 GAATGC GAATGC(1/-1)
Bsp1407I 1641 TGTACA T^GTACA
BspHI 1194 TCATGA T^CATGA
BsrDI 1301 GCAATG GCAATG(2/0)
BsrFI 582 GCCGGC [GA]^CCGG[CT]
BsrGI 1641 TGTACA T^GTACA
BssSI 339 CACGAG CACGAG(-5/-1)
BstAPI 1456 GCACAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 474 CCACCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1073 AGATCT [GA]^GATC[CT]
CfoI 586 GCGC GCG^C
Cfr10I 582 GCCGGC [GA]^CCGG[CT]
DraII 1235 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 1423 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1235 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 690 TGATCA T^GATCA
HaeII 585 GGCGCC [GA]GCGC^[CT]
HhaI 586 GCGC GCG^C
HinP1I 586 GCGC G^CGC
KasI 585 GGCGCC G^GCGCC
MfeI 1626 CAATTG C^AATTG
MflI 1073 AGATCT [GA]^GATC[CT]
MunI 1626 CAATTG C^AATTG
NaeI 582 GCCGGC GCC^GGC
NarI 585 GGCGCC GG^CGCC
NgoMIV 582 GCCGGC G^CCGGC
NspI 614 GCATGC [GA]CATG^[CT]
PshAI 155 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1194 TCATGA T^CATGA
RsaI 1642 GTAC GT^AC
SapI 931 GCTCTTC GCTCTTC(1/4)
SfoI 585 GGCGCC GGC^GCC
SphI 614 GCATGC GCATG^C
SspBI 1641 TGTACA T^GTACA
Tsp45I 563 GTGAC ^GT[GC]AC
XbaI 193 TCTAGA T^CTAGA
XhoII 1073 AGATCT [GA]^GATC[CT]
XmnI 1377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 226 GGACC G^G[AT]CC
335 GGACC
BstF5I 1593 CATCC GGATG(2/0)
1398 GGATG
EaeI 64 CGGCCA [CT]^GGCC[GA]
296 CGGCCA
FokI 1593 CATCC GGATG(9/13)
1398 GGATG
HapII 295 CCGG C^CGG
583 CCGG
HpaII 295 CCGG C^CGG
583 CCGG
KspI 221 CCGCGG CCGC^GG
259 CCGCGG
MaeIII 563 GTGAC ^GT[ACGT]AC
1329 GTTAC
MseI 680 TTAA T^TAA
721 TTAA
MspA1I 221 CCGCGG C[AC]G^C[GT]G
259 CCGCGG
MspI 295 CCGG C^CGG
583 CCGG
NcoI 67 CCATGG C^CATGG
1318 CCATGG
NdeI 839 CATATG CA^TATG
1759 CATATG
SacII 221 CCGCGG CCGC^GG
259 CCGCGG
SmlI 619 CTTGAG C^T[CT][GA]AG
1021 CTCAAG
SspI 98 AATATT AAT^ATT
760 AATATT
Tru9I 680 TTAA T^TAA
721 TTAA
TspRI 561 CAGTG CA[GC]TG[ACGT][ACGT]^
1386 CACTG
XcmI 200 CCAAGACGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1309 CCAGGAAGACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
ApoI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI HaeIII HgaI Hin1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV PflMI PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp509I Van91I