(Linear) (Six-base) MAPSORT of: KIEE6773.seq Check: 3664 from: 1 to: 1800 KIEE6773.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 252 1800 Size: 252 1548 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 620 676 1127 1184 1800 Size: 374 246 56 451 57 616 Fragments arranged by size: 616 451 374 246 57 56 AleI CACnn'nnGTG Cuts at: 0 343 479 1800 Size: 343 136 1321 Fragments arranged by size: 1321 343 136 AlwNI CAG_nnn'CTG Cuts at: 0 151 1800 Size: 151 1649 ApoI r'AATT_y Cuts at: 0 799 1114 1378 1469 1790 1800 Size: 799 315 264 91 321 10 Fragments arranged by size: 799 321 315 264 91 10 BanI G'GyrC_C Cuts at: 0 315 585 1273 1800 Size: 315 270 688 527 Fragments arranged by size: 688 527 315 270 BanII G_rGCy'C Cuts at: 0 125 187 269 598 1800 Size: 125 62 82 329 1202 Fragments arranged by size: 1202 329 125 82 62 BbeI G_GCGC'C Cuts at: 0 589 1800 Size: 589 1211 BbsI GAAGACnn'nnnn_ Cuts at: 0 557 664 1320 1800 Size: 557 107 656 480 Fragments arranged by size: 656 557 480 107 BclI T'GATC_A Cuts at: 0 690 1800 Size: 690 1110 BglI GCCn_nnn'nGGC Cuts at: 0 1243 1800 Size: 1243 557 BglII A'GATC_T Cuts at: 0 1073 1800 Size: 1073 727 Bme1580I G_kGCm'C Cuts at: 0 991 1800 Size: 991 809 BmgBI CAC'GTC Cuts at: 0 495 1488 1800 Size: 495 993 312 Fragments arranged by size: 993 495 312 BmrI ACTGGGnnnn_n' Cuts at: 0 1397 1800 Size: 1397 403 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 1800 Size: 570 1230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 859 995 1774 1800 Size: 859 136 779 26 Fragments arranged by size: 859 779 136 26 Bpu10I CC'TnA_GC Cuts at: 0 641 1800 Size: 641 1159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1006 1800 Size: 640 366 794 Fragments arranged by size: 794 640 366 BsaHI Gr'CG_yC Cuts at: 0 156 205 586 1800 Size: 156 49 381 1214 Fragments arranged by size: 1214 381 156 49 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 28 1800 Size: 28 1772 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 404 1546 1549 1800 Size: 404 1142 3 251 Fragments arranged by size: 1142 404 251 3 BseYI C'CCAG_C Cuts at: 0 358 610 1800 Size: 358 252 1190 Fragments arranged by size: 1190 358 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1331 1800 Size: 1331 469 BsiHKAI G_wGCw'C Cuts at: 0 1458 1800 Size: 1458 342 BsmI GAATG_Cn' Cuts at: 0 642 1800 Size: 642 1158 Bsp1286I G_dGCh'C Cuts at: 0 125 187 269 598 991 1458 1800 Size: 125 62 82 329 393 467 342 Fragments arranged by size: 467 393 342 329 125 82 62 BspHI T'CATG_A Cuts at: 0 1194 1800 Size: 1194 606 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 252 659 1353 1800 Size: 252 407 694 447 Fragments arranged by size: 694 447 407 252 BsrDI GCAATG_nn' Cuts at: 0 1308 1800 Size: 1308 492 BsrFI r'CCGG_y Cuts at: 0 582 1800 Size: 582 1218 BsrGI T'GTAC_A Cuts at: 0 1641 1800 Size: 1641 159 BssSI C'ACGA_G Cuts at: 0 339 1800 Size: 339 1461 BstAPI GCAn_nnn'nTGC Cuts at: 0 1462 1800 Size: 1462 338 BstXI CCAn_nnnn'nTGG Cuts at: 0 481 1800 Size: 481 1319 BstYI r'GATC_y Cuts at: 0 1073 1800 Size: 1073 727 BtgI C'CryG_G Cuts at: 0 67 221 259 1318 1800 Size: 67 154 38 1059 482 Fragments arranged by size: 1059 482 154 67 38 EaeI y'GGCC_r Cuts at: 0 64 296 1800 Size: 64 232 1504 Fragments arranged by size: 1504 232 64 EarI CTCTTCn'nnn_ Cuts at: 0 276 938 1130 1142 1160 1514 1535 1800 Size: 276 662 192 12 18 354 21 265 Fragments arranged by size: 662 354 276 265 192 21 18 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 620 676 859 995 1127 1184 1774 Size: 374 246 56 183 136 132 57 590 Cuts at: 1774 1800 Size: 26 Fragments arranged by size: 590 374 246 183 136 132 57 56 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 1427 1800 Size: 1427 373 EcoO109I rG'GnC_Cy Cuts at: 0 1236 1800 Size: 1236 564 HaeII r_GCGC'y Cuts at: 0 589 1800 Size: 589 1211 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 30 97 129 293 325 354 386 1065 Size: 30 67 32 164 32 29 32 679 Cuts at: 1065 1097 1800 Size: 32 703 Fragments arranged by size: 703 679 164 67 32 32 32 32 30 29 HincII GTy'rAC Cuts at: 0 710 747 897 1800 Size: 710 37 150 903 Fragments arranged by size: 903 710 150 37 KasI G'GCGC_C Cuts at: 0 585 1800 Size: 585 1215 MfeI C'AATT_G Cuts at: 0 1626 1800 Size: 1626 174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 308 859 1765 1800 Size: 308 551 906 35 Fragments arranged by size: 906 551 308 35 MslI CAynn'nnrTG Cuts at: 0 343 479 1199 1597 1800 Size: 343 136 720 398 203 Fragments arranged by size: 720 398 343 203 136 MspA1I CmG'CkG Cuts at: 0 223 261 1800 Size: 223 38 1539 Fragments arranged by size: 1539 223 38 NaeI GCC'GGC Cuts at: 0 584 1800 Size: 584 1216 NarI GG'CG_CC Cuts at: 0 586 1800 Size: 586 1214 NcoI C'CATG_G Cuts at: 0 67 1318 1800 Size: 67 1251 482 Fragments arranged by size: 1251 482 67 NdeI CA'TA_TG Cuts at: 0 840 1760 1800 Size: 840 920 40 Fragments arranged by size: 920 840 40 NgoMIV G'CCGG_C Cuts at: 0 582 1800 Size: 582 1218 NspI r_CATG'y Cuts at: 0 618 1800 Size: 618 1182 PflMI CCAn_nnn'nTGG Cuts at: 0 151 906 1747 1800 Size: 151 755 841 53 Fragments arranged by size: 841 755 151 53 PshAI GACnn'nnGTC Cuts at: 0 159 1800 Size: 159 1641 SacII CC_GC'GG Cuts at: 0 224 262 1800 Size: 224 38 1538 Fragments arranged by size: 1538 224 38 SapI GCTCTTCn'nnn_ Cuts at: 0 938 1800 Size: 938 862 SfoI GGC'GCC Cuts at: 0 587 1800 Size: 587 1213 SmlI C'TyrA_G Cuts at: 0 619 1021 1800 Size: 619 402 779 Fragments arranged by size: 779 619 402 SphI G_CATG'C Cuts at: 0 618 1800 Size: 618 1182 SspI AAT'ATT Cuts at: 0 100 762 1800 Size: 100 662 1038 Fragments arranged by size: 1038 662 100 StyI C'CwwG_G Cuts at: 0 67 125 178 1318 1800 Size: 67 58 53 1140 482 Fragments arranged by size: 1140 482 67 58 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 202 1058 1311 1800 Size: 202 856 253 489 Fragments arranged by size: 856 489 253 202 TatI w'GTAC_w Cuts at: 0 1641 1800 Size: 1641 159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 327 359 1800 Size: 327 32 1441 Fragments arranged by size: 1441 327 32 XbaI T'CTAG_A Cuts at: 0 193 1800 Size: 193 1607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 207 1316 1800 Size: 207 1109 484 Fragments arranged by size: 1109 484 207 XmnI GAAnn'nnTTC Cuts at: 0 1381 1800 Size: 1381 419 Enzymes that do cut: AarI AcuI AleI AlwNI ApoI BanI BanII BbeI BbsI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PshAI SacII SapI SfoI SmlI SphI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BlpI BmtI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MluI MscI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI ZraI