Restriction map for
pFN21AE6773
(Circular) (Six-base) MAPSORT of: pFN21AE6773.seq Check: 7988 from: 1 to: 6505
pFN21AE6773 6505 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2257 2257
Size: 6505
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3848 1937
Size: 1911 4594
Acc65I G'GTAC_C
Cuts at: 3826 3826
Size: 6505
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2625 2681 3132 3189 4568 5952 6220
Size: 747 246 56 451 57 1379 1384 268
Cuts at: 6220 1632
Size: 1917
Fragments arranged by size:
1917 1384 1379 747 451 268 246 57
56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6308
AflIII A'CryG_T
Cuts at: 1203 5404 6381 1203
Size: 4201 977 1327
Fragments arranged by size:
4201 1327 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 2348 2484 1166
Size: 1182 136 5187
Fragments arranged by size:
5187 1182 136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6505
AlwNI CAG_nnn'CTG
Cuts at: 2156 4248 5820 2156
Size: 2092 1572 2841
Fragments arranged by size:
2841 2092 1572
ApaLI G'TGCA_C
Cuts at: 4562 5718 4562
Size: 1156 5349
ApoI r'AATT_y
Cuts at: 1493 2804 3119 3383 3474 3795 3814 4040 4192
Size: 1311 315 264 91 321 19 226 152
Cuts at: 4192 6301 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1311 321 315 264 226 152
91 19
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 3830 1870
Size: 73 1887 4545
Fragments arranged by size:
4545 1887 73
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 2590 3278 3826 5281
Size: 325 217 627 533 270 688 548 1455
Cuts at: 5281 618
Size: 1842
Fragments arranged by size:
1842 1455 688 627 548 533 325 270
217
BanII G_rGCy'C
Cuts at: 1391 2130 2192 2274 2603 3824 1391
Size: 739 62 82 329 1221 4072
Fragments arranged by size:
4072 1221 739 329 82 62
BbeI G_GCGC'C
Cuts at: 2594 2594
Size: 6505
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2562 2669 3325 928
Size: 971 663 107 656 4108
Fragments arranged by size:
4108 971 663 656 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4696 4730 1903
Size: 34 2759 34 3678
Fragments arranged by size:
3678 2759 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 5358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3248 5116 136
Size: 107 122 71 631 605 1576 1868 1525
Fragments arranged by size:
1868 1576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3078 6500 3078
Size: 3422 3083
BlpI GC'TnA_GC
Cuts at: 3922 3922
Size: 6505
Bme1580I G_kGCm'C
Cuts at: 1790 2996 4566 5722 1790
Size: 1206 1570 1156 2573
Fragments arranged by size:
2573 1570 1206 1156
BmgBI CAC'GTC
Cuts at: 1318 2500 3493 1318
Size: 1182 993 4330
Fragments arranged by size:
4330 1182 993
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4236 5194
Size: 416 53 83 399 24 1985 834 958
Cuts at: 5194 442
Size: 1753
Fragments arranged by size:
1985 1753 958 834 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 6505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 3000 3779 5165 1239
Size: 1625 136 779 1386 2579
Fragments arranged by size:
2579 1625 1386 779 136
Bpu10I CC'TnA_GC
Cuts at: 2646 2646
Size: 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3011 4630 5495 5793 2645
Size: 366 1619 865 298 3357
Fragments arranged by size:
3357 1619 865 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5168 882
Size: 617 3669 2219
Fragments arranged by size:
3669 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4296 493
Size: 711 3092 2702
Fragments arranged by size:
3092 2702 711
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2161 2210 2591 4312
Size: 53 83 186 936 628 49 381 1721
Cuts at: 4312 4694 5329 275
Size: 382 635 1451
Fragments arranged by size:
1721 1451 936 635 628 382 381 186
83 53 49
BsaWI w'CCGG_w
Cuts at: 4938 5336 5610 5757 6168 4938
Size: 398 274 147 411 5275
Fragments arranged by size:
5275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 5650
Fragments arranged by size:
5650 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2409 3551 3554 1186
Size: 1223 1142 3 4137
Fragments arranged by size:
4137 1223 1142 3
BseYI C'CCAG_C
Cuts at: 2363 2615 5708 6204 2363
Size: 252 3093 496 2664
Fragments arranged by size:
3093 2664 496 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3336 1163
Size: 573 1600 4332
Fragments arranged by size:
4332 1600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3955 4716 5744 1540
Size: 275 186 1954 761 1028 2301
Fragments arranged by size:
2301 1954 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3463 4566 4651 5722 3463
Size: 1103 85 1071 4246
Fragments arranged by size:
4246 1103 1071 85
BsmI GAATG_Cn'
Cuts at: 2647 4016 4109 2647
Size: 1369 93 5043
Fragments arranged by size:
5043 1369 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2192 2274 2603 2996 3463 3824
Size: 399 340 62 82 329 393 467 361
Cuts at: 3824 4566 4651 5722 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 393 361 340
329 85 82 62
BspEI T'CCGG_A
Cuts at: 6168 6168
Size: 6505
BspHI T'CATG_A
Cuts at: 3199 4394 6124 3199
Size: 1195 1730 3580
Fragments arranged by size:
3580 1730 1195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 2664 3358 3860 844
Size: 1413 407 694 502 3489
Fragments arranged by size:
3489 1413 694 502 407
BsrBI CCG'CTC
Cuts at: 1394 1823 4392 1394
Size: 429 2569 3507
Fragments arranged by size:
3507 2569 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3313 5000 5174 65
Size: 1183 2065 1687 174 1396
Fragments arranged by size:
2065 1687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2587 5149 5336 1700
Size: 110 777 2562 187 2869
Fragments arranged by size:
2869 2562 777 187 110
BsrGI T'GTAC_A
Cuts at: 96 3646 96
Size: 3550 2955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6505
BssSI C'ACGA_G
Cuts at: 2344 4565 5577 2344
Size: 2221 1012 3272
Fragments arranged by size:
3272 2221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3467 794
Size: 2673 3832
BstBI TT'CG_AA
Cuts at: 5309 5309
Size: 6505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6505
BstXI CCAn_nnnn'nTGG
Cuts at: 2486 2486
Size: 6505
BstYI r'GATC_y
Cuts at: 1972 3078 3835 4202 4587 4604 6056 6142 6154
Size: 1106 757 367 385 17 1452 86 12
Cuts at: 6154 6500 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1106 757 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 2226 2264 3323 513
Size: 552 939 68 154 38 1059 3695
Fragments arranged by size:
3695 1059 939 552 154 68 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 4021 4815 4835 4021
Size: 794 20 5691
Fragments arranged by size:
5691 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 3809 3809
Size: 6505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5512 809
Size: 507 4196 1802
Fragments arranged by size:
4196 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2301 3952
Size: 54 1425 50 275 18 239 232 1651
Cuts at: 3952 4841 6470 8
Size: 889 1629 43
Fragments arranged by size:
1651 1629 1425 889 275 239 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3952 1537
Size: 275 2140 4090
Fragments arranged by size:
4090 2140 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2281 2943 3135 3147 3165 3519 3540 4435
Size: 928 662 192 12 18 354 21 895
Cuts at: 4435 1353
Size: 3423
Fragments arranged by size:
3423 928 895 662 354 192 21 18
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5092 5466 5612 387
Size: 1064 138 3503 374 146 1280
Fragments arranged by size:
3503 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2625 2681 2864 3000 3132 3189
Size: 393 747 246 56 183 136 132 57
Cuts at: 3189 3779 4568 5165 5952 6220 1239
Size: 590 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 747 597 590 393 268
246 183 136 132 57 56
EcoNI CCTnn'n_nnAGG
Cuts at: 3432 3432
Size: 6505
EcoO109I rG'GnC_Cy
Cuts at: 3241 3241
Size: 6505
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2594 5652 1678
Size: 916 3058 2531
Fragments arranged by size:
3058 2531 916
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2102 2134 2298
Size: 32 135 32 431 32 67 32 164
Cuts at: 2298 2330 2359 2391 3070 3102 5146 5178 5220
Size: 32 29 32 679 32 2044 32 42
Cuts at: 5220 5252 1373
Size: 32 2626
Fragments arranged by size:
2626 2044 679 431 164 135 67 42
32 32 32 32 32 32 32 32
32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2715 2752 2902 3849 4095 669
Size: 988 281 777 37 150 947 246 3079
Fragments arranged by size:
3079 988 947 777 281 246 150 37
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6505
HpaI GTT'AAC
Cuts at: 4095 4095
Size: 6505
KasI G'GCGC_C
Cuts at: 2590 2590
Size: 6505
KpnI G_GTAC'C
Cuts at: 3830 3830
Size: 6505
MfeI C'AATT_G
Cuts at: 3631 4104 3631
Size: 473 6032
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 2864 3770 5619 5803 1305
Size: 1008 551 906 1849 184 2007
Fragments arranged by size:
2007 1849 1008 906 551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2484 3204 3602 4463 4822 4981
Size: 648 1182 136 720 398 861 359 159
Cuts at: 4981 518
Size: 2042
Fragments arranged by size:
2042 1182 861 720 648 398 359 159
136
MspA1I CmG'CkG
Cuts at: 1606 2228 2266 3921 4598 5746 5991 1606
Size: 622 38 1655 677 1148 245 2120
Fragments arranged by size:
2120 1655 1148 677 622 245 38
NaeI GCC'GGC
Cuts at: 2589 2589
Size: 6505
NarI GG'CG_CC
Cuts at: 2591 2591
Size: 6505
NcoI C'CATG_G
Cuts at: 513 1065 2004 2072 3323 513
Size: 552 939 68 1251 3695
Fragments arranged by size:
3695 1251 939 552 68
NdeI CA'TA_TG
Cuts at: 387 2845 3765 387
Size: 2458 920 3127
Fragments arranged by size:
3127 2458 920
NgoMIV G'CCGG_C
Cuts at: 2587 2587
Size: 6505
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 2623 3863 5408 1132
Size: 97 1394 1240 1545 2229
Fragments arranged by size:
2229 1545 1394 1240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6505
PciI A'CATG_T
Cuts at: 5404 5404
Size: 6505
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2156 2911 3752 1108
Size: 1048 755 841 3861
Fragments arranged by size:
3861 1048 841 755
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2164 1613
Size: 551 5954
PsiI TTA'TAA
Cuts at: 4075 4075
Size: 6505
PstI C_TGCA'G
Cuts at: 830 3857 830
Size: 3027 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
SacII CC_GC'GG
Cuts at: 2229 2267 2229
Size: 38 6467
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SapI GCTCTTCn'nnn_
Cuts at: 2943 2943
Size: 6505
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SfcI C'TryA_G
Cuts at: 826 1045 3801 3853 4988 5669 5860 826
Size: 219 2756 52 1135 681 191 1471
Fragments arranged by size:
2756 1471 1135 681 219 191 52
SfoI GGC'GCC
Cuts at: 2592 2592
Size: 6505
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 3832 1872
Size: 1960 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 3026 4609 5510 5772 820
Size: 197 926 681 402 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 681 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 2623 3863 1132
Size: 1491 1240 3774
Fragments arranged by size:
3774 1491 1240
SspI AAT'ATT
Cuts at: 5 52 2105 2767 4429 5
Size: 47 2053 662 1662 2081
Fragments arranged by size:
2081 2053 1662 662 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6505
StyI C'CwwG_G
Cuts at: 513 1065 2004 2072 2130 2183 3323 3944 513
Size: 552 939 68 58 53 1140 621 3074
Fragments arranged by size:
3074 1140 939 621 552 68 58 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 3063 3316 4532 4549 4702 4887
Size: 1255 627 856 253 1216 17 153 185
Cuts at: 4887 5332 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1216 856 627 445 253 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3646 4751 6463
Size: 274 80 33 51 1443 1669 1105 1712
Cuts at: 6463 96
Size: 138
Fragments arranged by size:
1712 1669 1443 1105 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2332 2364 6465 6497 2332
Size: 32 4101 32 2340
Fragments arranged by size:
4101 2340 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6505
XbaI T'CTAG_A
Cuts at: 2198 3841 2198
Size: 1643 4862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 3321 2212
Size: 1109 5396
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6505
XmaI C'CCGG_G
Cuts at: 1870 3830 1870
Size: 1960 4545
XmnI GAAnn'nnTTC
Cuts at: 3386 4634 3386
Size: 1248 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SfiI SrfI SwaI