ASB12 ORF clone pFN21AE6693

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Halotag ORF Clone pFN21AE6693

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Clone info.

Species

human

Clone Name

pFN21AE6693 Sequence (with vector) Restriction Map

Product ID

FHC27413

ORF Sequence

DNA sequence (954 bp)
Protein sequence (318 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6693

Symbol

ASB12

Description

ankyrin repeat and SOCS box containing 12

Original Clone ID

cp96693

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 954 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 318 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14378.2 3.6e-105 100.0 ASB12 CCDS33416.1 5.3e-25 39.8 ASB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002110 75 103 PF00023 Ankyrin repeat IPR002110 107 132 PF00023 Ankyrin repeat IPR002110 184 208 PF00023 Ankyrin repeat IPR001496 277 317 PF07525 SOCS protein HMMSmart IPR002110 75 101 SM00248 Ankyrin repeat IPR002110 105 134 SM00248 Ankyrin repeat IPR002110 138 167 SM00248 Ankyrin repeat IPR002110 180 209 SM00248 Ankyrin repeat IPR002110 226 252 SM00248 Ankyrin repeat IPR001496 277 317 SM00969 SOCS protein ProfileScan IPR002110 75 104 PS50088 Ankyrin repeat IPR020683 75 255 PS50297 Ankyrin repeat-containing domain IPR002110 105 131 PS50088 Ankyrin repeat IPR002110 138 170 PS50088 Ankyrin repeat IPR002110 184 212 PS50088 Ankyrin repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_569059 318 300891 100.0 100.0 NP_001035535 335 605758 39.8 89.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6693 Download

>KIEE6693  954 bp
ATGAGAATAGTTCTCCAATTAGCCAAGATGAACCTCATGGACATCACCAAGATCTTCTCC
CTCCTGCAGCCCGACAAGGAGGAGGAGGACACTGACACAGAGGAGAAGCAGGCTCTCAAT
CAAGCAGTGTATGACAACGACTCCTATACTTTGGACCAGCTTTTGCGCCAGGAGCGTTAC
AAACGTTTCATCAACAGCAGGAGTGGCTGGGGTGTTCCTGGGACACCCTTGCGCTTGGCT
GCTTCTTATGGCCACTTGAGCTGTTTGCAAGTCCTCTTAGCCCATGGTGCTGATGTTGAC
AGCTTGGATGTCAAGGCACAGACGCCACTTTTCACTGCTGTCAGTCATGGCCATCTGGAC
TGTGTACGTGTGCTTTTGGAAGCTGGTGCCTCTCCTGGTGGTAGCATCTACAACAACTGT
TCTCCCGTGCTCACAGCTGCCCGTGATGGTGCTGTTGCTATCCTGCAGGAGCTCCTAGAC
CATGGTGCAGAGGCCAACGTCAAAGCTAAACTACCAGTCTGGGCATCAAACATAGCTTCA
TGTTCTGGCCCCCTCTATTTGGCCGCAGTCTACGGGCACCTGGACTGTTTCCGCCTGCTT
TTGCTCCACGGGGCAGACCCTGACTACAACTGCACTGACCAGGGCCTATTGGCTCGTGTC
CCAAGACCCCGCACCCTCCTTGAAATCTGCCTCCATCATAATTGTGAGCCAGAGTATATC
CAGCTGTTAATCGATTTTGGTGCTAATATCTACCTTCCATCTCTCTCCCTTGACCTGACC
TCACAAGATGATAAAGGCATTGCATTGCTGCTACAGGCCCGAGCCACTCCACGGTCACTT
CTATCACAGGTCCGTTTAGTCGTCCGCAGAGCCTTGTGCCAGGCTGGCCAGCCACAAGCC
ATCAACCAGCTGGATATTCCTCCCATGTTGATTAGCTACCTAAAACACCAACTG

ORF Protein

Cloned ORF protein sequence for pFN21AE6693 Download

>KIEE6693  318 aa
MRIVLQLAKMNLMDITKIFSLLQPDKEEEDTDTEEKQALNQAVYDNDSYTLDQLLRQERY
KRFINSRSGWGVPGTPLRLAASYGHLSCLQVLLAHGADVDSLDVKAQTPLFTAVSHGHLD
CVRVLLEAGASPGGSIYNNCSPVLTAARDGAVAILQELLDHGAEANVKAKLPVWASNIAS
CSGPLYLAAVYGHLDCFRLLLLHGADPDYNCTDQGLLARVPRPRTLLEICLHHNCEPEYI
QLLIDFGANIYLPSLSLDLTSQDDKGIALLLQARATPRSLLSQVRLVVRRALCQAGQPQA
INQLDIPPMLISYLKHQL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6693 Download

>pFN21AE6693  5659 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGAATAGTTCTCCAATTAGCCAAGATGAACCT
CATGGACATCACCAAGATCTTCTCCCTCCTGCAGCCCGACAAGGAGGAGGAGGACACTGA
CACAGAGGAGAAGCAGGCTCTCAATCAAGCAGTGTATGACAACGACTCCTATACTTTGGA
CCAGCTTTTGCGCCAGGAGCGTTACAAACGTTTCATCAACAGCAGGAGTGGCTGGGGTGT
TCCTGGGACACCCTTGCGCTTGGCTGCTTCTTATGGCCACTTGAGCTGTTTGCAAGTCCT
CTTAGCCCATGGTGCTGATGTTGACAGCTTGGATGTCAAGGCACAGACGCCACTTTTCAC
TGCTGTCAGTCATGGCCATCTGGACTGTGTACGTGTGCTTTTGGAAGCTGGTGCCTCTCC
TGGTGGTAGCATCTACAACAACTGTTCTCCCGTGCTCACAGCTGCCCGTGATGGTGCTGT
TGCTATCCTGCAGGAGCTCCTAGACCATGGTGCAGAGGCCAACGTCAAAGCTAAACTACC
AGTCTGGGCATCAAACATAGCTTCATGTTCTGGCCCCCTCTATTTGGCCGCAGTCTACGG
GCACCTGGACTGTTTCCGCCTGCTTTTGCTCCACGGGGCAGACCCTGACTACAACTGCAC
TGACCAGGGCCTATTGGCTCGTGTCCCAAGACCCCGCACCCTCCTTGAAATCTGCCTCCA
TCATAATTGTGAGCCAGAGTATATCCAGCTGTTAATCGATTTTGGTGCTAATATCTACCT
TCCATCTCTCTCCCTTGACCTGACCTCACAAGATGATAAAGGCATTGCATTGCTGCTACA
GGCCCGAGCCACTCCACGGTCACTTCTATCACAGGTCCGTTTAGTCGTCCGCAGAGCCTT
GTGCCAGGCTGGCCAGCCACAAGCCATCAACCAGCTGGATATTCCTCCCATGTTGATTAG
CTACCTAAAACACCAACTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT