################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 568 GTCTAC GT^[AC][GT]AC AclI 182 AACGTT AA^CGTT AcyI 321 GACGCC G[GA]^CG[CT]C AfaI 364 GTAC GT^AC AflIII 366 ACGTGT A^C[GA][CT]GT AlwNI 614 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 818 CCCGAG C^[CT]CG[GA]G BanII 469 GAGCTC G[GA]GC[CT]^C BcgI 732 CGATTTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 475 CTAG C^TAG BglI 878 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 50 AGATCT A^GATCT BsaAI 365 TACGTG [CT]AC^GT[GA] BsaHI 321 GACGCC G[GA]^CG[CT]C BsoBI 818 CCCGAG C^[CT]CG[GA]G BspDI 730 ATCGAT AT^CGAT BsrI 514 CCAGT ACTGG(1/-1) BssSI 653 CTCGTG CACGAG(-5/-1) BstF5I 306 GGATG GGATG(2/0) BstYI 50 AGATCT [GA]^GATC[CT] ClaI 730 ATCGAT AT^CGAT DdeI 276 CTTAG C^T[ACGT]AG DpnII 51 GATC ^GATC DraII 641 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 641 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 668 CCCGC CCCGC(4/6) FokI 306 GGATG GGATG(9/13) HgaI 321 GACGC GACGC(5/10) Hin1I 321 GACGCC G[GA]^CG[CT]C HincII 295 GTTGAC GT[CT]^[GA]AC HindII 295 GTTGAC GT[CT]^[GA]AC HinfI 139 GACTC G^A[ACGT]TC HphI 44 TCACC GGTGA(8/7) MaeI 475 CTAG C^TAG MboI 51 GATC ^GATC MboII 53 TCTTC GAAGA(8/7) MflI 50 AGATCT [GA]^GATC[CT] MseI 727 TTAA T^TAA MslI 697 CATAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 51 GATC ^GATC PleI 139 GACTC GAGTC(4/5) Psp1406I 182 AACGTT AA^CGTT RsaI 364 GTAC GT^AC SacI 469 GAGCTC GAGCT^C Sau3AI 51 GATC ^GATC SbfI 462 CCTGCAGG CCTGCA^GG SmlI 255 CTTGAG C^T[CT][GA]AG Sse8387I 462 CCTGCAGG CCTGCA^GG TaqI 731 TCGA T^CGA Tru9I 727 TTAA T^TAA Tsp45I 834 GTCAC ^GT[GC]AC TthHB8I 731 TCGA T^CGA XcmI 899 CCATCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 50 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 427 GTGCTC G[AT]GC[AT]^C 469 GAGCTC AvaII 153 GGACC G^G[AT]CC 849 GGTCC BanI 385 GGTGCC G^G[CT][GA]CC 575 GGCACC BsgI 630 CTGCAC GTGCAG(16/14) 485 GTGCAG BsiHKAI 427 GTGCTC G[AT]GC[AT]^C 469 GAGCTC BsiYI 552 CCTCTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 824 CCACTCCACGG BslI 552 CCTCTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 824 CCACTCCACGG BsmFI 220 GGGAC GGGAC(10/14) 658 GTCCC BsrDI 798 CATTGC GCAATG(2/0) 803 CATTGC CfoI 165 GCGC GCG^C 231 GCGC EcoT14I 282 CCATGG C^C[AT][AT]GG 480 CCATGG HhaI 165 GCGC GCG^C 231 GCGC HinP1I 165 GCGC G^CGC 231 GCGC MaeIII 176 GTTAC ^GT[ACGT]AC 834 GTCAC NcoI 282 CCATGG C^CATGG 480 CCATGG PstI 64 CTGCAG CTGCA^G 463 CTGCAG SfaNI 404 GCATC GCATC(5/9) 523 GCATC StyI 282 CCATGG C^C[AT][AT]GG 480 CCATGG Tsp509I 17 AATT ^AATT 700 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI BalI BbvI BmyI BsaJI BseRI Bsp1286I BssKI BstNI Cac8I Cfr13I DsaI EaeI EcoRII Fnu4HI HaeIII ItaI MaeII MluNI MnlI MscI MspA1I MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI SfcI TseI TspRI