Restriction map for
pFN21AE6693
(Circular) (Six-base) MAPSORT of: pFN21AE6693.seq Check: 9652 from: 1 to: 5659
pFN21AE6693 5659 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3002 1937
Size: 637 428 4594
Fragments arranged by size:
4594 637 428
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 2188 3786 4159 1563
Size: 625 1598 373 3063
Fragments arranged by size:
3063 1598 625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3722 5106 5374 1632
Size: 2090 1384 268 1917
Fragments arranged by size:
2090 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 2371 4558 5535 1203
Size: 1168 2187 977 1327
Fragments arranged by size:
2187 1327 1168 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2825 1184
Size: 1641 4018
AlwNI CAG_nnn'CTG
Cuts at: 2624 3402 4974 2624
Size: 778 1572 3309
Fragments arranged by size:
3309 1572 778
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2823 2984 1870
Size: 73 880 161 4545
Fragments arranged by size:
4545 880 161 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2580 2980 4435 618
Size: 325 217 627 603 190 400 1455 1842
Fragments arranged by size:
1842 1455 627 603 400 325 217 190
BanII G_rGCy'C
Cuts at: 1391 2478 2978 1391
Size: 1087 500 4072
Fragments arranged by size:
4072 1087 500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2726 2760 3850 3884 1903
Size: 34 789 34 1090 34 3678
Fragments arranged by size:
3678 1090 789 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2889 4270 136
Size: 107 122 71 631 605 1217 1381 1525
Fragments arranged by size:
1525 1381 1217 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2055 5654 2055
Size: 3599 2060
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2583 3720 4876 1790
Size: 793 1137 1156 2573
Fragments arranged by size:
2573 1156 1137 793
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4319 1239
Size: 3080 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 3784 4649 4947 2281
Size: 1503 865 298 2993
Fragments arranged by size:
2993 1503 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2372 3450 493
Size: 711 1168 1078 2702
Fragments arranged by size:
2702 1168 1078 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2327 3466 3848 4483
Size: 53 83 186 936 794 1139 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1139 936 794 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 1178
Size: 30 165 30 726 30 4678
Fragments arranged by size:
4678 726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2102 2120 1186
Size: 913 3 18 4725
Fragments arranged by size:
4725 913 18 3
BseYI C'CCAG_C
Cuts at: 2211 4862 5358 2211
Size: 2651 496 2512
Fragments arranged by size:
2651 2512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2511 2620 1163
Size: 573 775 109 4202
Fragments arranged by size:
4202 775 573 109
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 2478 3720 3805 4876 2436
Size: 42 1242 85 1071 3219
Fragments arranged by size:
3219 1242 1071 85 42
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2436 2478 2583 2978 3720 3805 4876
Size: 399 646 42 105 395 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 646 399 395 105 85
42
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2802 2807 4154 4328 65
Size: 1183 1554 5 1347 174 1396
Fragments arranged by size:
1554 1396 1347 1183 174 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 2658 3719 4731 2658
Size: 1061 1012 3586
Fragments arranged by size:
3586 1061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2055 2989 3356 3741 3758 5210 5296 5308
Size: 83 934 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 934 385 367 346 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2287 2485 2611 2834 513
Size: 552 1222 198 126 223 3338
Fragments arranged by size:
3338 1222 552 223 198 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2136 2337 3175 3969 3989 2136
Size: 201 838 794 20 3806
Fragments arranged by size:
3806 838 794 201 20
ClaI AT'CG_AT
Cuts at: 1554 2736 1554
Size: 1182 4477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2254 2353 2565
Size: 54 1425 50 275 18 424 99 212
Cuts at: 2565 2890 3106 3995 5624 8
Size: 325 216 889 1629 43
Fragments arranged by size:
1629 1425 889 424 325 275 216 212
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3589 1353
Size: 2236 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 4246 4620 4766 387
Size: 1064 138 996 1661 374 146 1280
Fragments arranged by size:
1661 1280 1064 996 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3722 4319 5106 5374 1239
Size: 393 2090 597 787 268 1524
Fragments arranged by size:
2090 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2647 2647
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2768 2800 4300
Size: 32 135 32 557 32 607 32 1500
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1500 607 557 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 3003 3249 669
Size: 988 281 364 701 246 3079
Fragments arranged by size:
3079 988 701 364 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2256 2355 2892 10
Size: 54 1425 343 424 99 537 2777
Fragments arranged by size:
2777 1425 537 424 343 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 3617 3976 4135 518
Size: 648 1540 911 359 159 2042
Fragments arranged by size:
2042 1540 911 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 2728 2914 3075 3752 4900 5145 1606
Size: 835 287 186 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 287 245 186 161
NcoI C'CATG_G
Cuts at: 513 1065 2287 2485 513
Size: 552 1222 198 3687
Fragments arranged by size:
3687 1222 552 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2073 2472 3011 830
Size: 1243 399 539 3478
Fragments arranged by size:
3478 1243 539 399
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2441 2728 2914 2441
Size: 287 186 5186
Fragments arranged by size:
5186 287 186
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 2472 3011 2472
Size: 539 5120
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2069 2468 2816 3007 4142 4823 5014
Size: 219 1024 399 348 191 1135 681 191
Cuts at: 5014 826
Size: 1471
Fragments arranged by size:
1471 1135 1024 681 399 348 219 191
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2260 3763 4664 4926 820
Size: 197 926 317 1503 901 262 1553
Fragments arranged by size:
1553 1503 926 901 317 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2287 2485 3098 513
Size: 552 1222 198 613 3074
Fragments arranged by size:
3074 1222 613 552 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 2911
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI