(Linear) (Six-base) MAPSORT of: KIEE6693.seq Check: 11 from: 1 to: 954 KIEE6693.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AccI GT'mk_AC Cuts at: 0 569 954 Size: 569 385 AclI AA'CG_TT Cuts at: 0 183 954 Size: 183 771 AflIII A'CryG_T Cuts at: 0 366 954 Size: 366 588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 820 954 Size: 820 134 AlwNI CAG_nnn'CTG Cuts at: 0 619 954 Size: 619 335 AvaI C'yCGr_G Cuts at: 0 818 954 Size: 818 136 BanI G'GyrC_C Cuts at: 0 385 575 954 Size: 385 190 379 Fragments arranged by size: 385 379 190 BanII G_rGCy'C Cuts at: 0 473 954 Size: 473 481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 721 755 954 Size: 721 34 199 Fragments arranged by size: 721 199 34 BglI GCCn_nnn'nGGC Cuts at: 0 884 954 Size: 884 70 BglII A'GATC_T Cuts at: 0 50 954 Size: 50 904 Bme1580I G_kGCm'C Cuts at: 0 578 954 Size: 578 376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 276 954 Size: 276 678 BsaAI yAC'GTr Cuts at: 0 367 954 Size: 367 587 BsaHI Gr'CG_yC Cuts at: 0 322 954 Size: 322 632 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 124 154 954 Size: 124 30 800 Fragments arranged by size: 800 124 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 97 115 954 Size: 94 3 18 839 Fragments arranged by size: 839 94 18 3 BseYI C'CCAG_C Cuts at: 0 206 954 Size: 206 748 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 506 615 954 Size: 506 109 339 Fragments arranged by size: 506 339 109 BsiHKAI G_wGCw'C Cuts at: 0 431 473 954 Size: 431 42 481 Fragments arranged by size: 481 431 42 Bsp1286I G_dGCh'C Cuts at: 0 431 473 578 954 Size: 431 42 105 376 Fragments arranged by size: 431 376 105 42 BsrDI GCAATG_nn' Cuts at: 0 797 802 954 Size: 797 5 152 Fragments arranged by size: 797 152 5 BssSI C'ACGA_G Cuts at: 0 653 954 Size: 653 301 BstYI r'GATC_y Cuts at: 0 50 954 Size: 50 904 BtgI C'CryG_G Cuts at: 0 282 480 606 829 954 Size: 282 198 126 223 125 Fragments arranged by size: 282 223 198 126 125 BtsI GCAGTG_nn' Cuts at: 0 131 332 954 Size: 131 201 622 Fragments arranged by size: 622 201 131 ClaI AT'CG_AT Cuts at: 0 731 954 Size: 731 223 EaeI y'GGCC_r Cuts at: 0 249 348 560 885 954 Size: 249 99 212 325 69 Fragments arranged by size: 325 249 212 99 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 580 954 Size: 580 374 EcoICRI GAG'CTC Cuts at: 0 471 954 Size: 471 483 EcoO109I rG'GnC_Cy Cuts at: 0 642 954 Size: 642 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 763 795 954 Size: 124 32 607 32 159 Fragments arranged by size: 607 159 124 32 32 HincII GTy'rAC Cuts at: 0 297 954 Size: 297 657 MscI TGG'CCA Cuts at: 0 251 350 887 954 Size: 251 99 537 67 Fragments arranged by size: 537 251 99 67 MslI CAynn'nnrTG Cuts at: 0 701 954 Size: 701 253 MspA1I CmG'CkG Cuts at: 0 436 723 909 954 Size: 436 287 186 45 Fragments arranged by size: 436 287 186 45 NcoI C'CATG_G Cuts at: 0 282 480 954 Size: 282 198 474 Fragments arranged by size: 474 282 198 PstI C_TGCA'G Cuts at: 0 68 467 954 Size: 68 399 487 Fragments arranged by size: 487 399 68 PvuII CAG'CTG Cuts at: 0 436 723 909 954 Size: 436 287 186 45 Fragments arranged by size: 436 287 186 45 SacI G_AGCT'C Cuts at: 0 473 954 Size: 473 481 SbfI CC_TGCA'GG Cuts at: 0 467 954 Size: 467 487 SfcI C'TryA_G Cuts at: 0 64 463 811 954 Size: 64 399 348 143 Fragments arranged by size: 399 348 143 64 SmlI C'TyrA_G Cuts at: 0 255 954 Size: 255 699 StyI C'CwwG_G Cuts at: 0 282 480 954 Size: 282 198 474 Fragments arranged by size: 474 282 198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 906 954 Size: 906 48 Enzymes that do cut: AccI AclI AflIII AlfI AlwNI AvaI BanI BanII BcgI BglI BglII Bme1580I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BssSI BstYI BtgI BtsI ClaI EaeI EciI EcoICRI EcoO109I Hin4I HincII MscI MslI MspA1I NcoI PstI PvuII SacI SbfI SfcI SmlI StyI XcmI Enzymes that do not cut: AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI