SLC8B1 ORF clone pFN21AE6486

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Halotag ORF Clone pFN21AE6486

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Clone info.

Species

human

Clone Name

pFN21AE6486 Sequence (with vector) Restriction Map

Product ID

FHC28183

ORF Sequence

DNA sequence (1752 bp)
Protein sequence (584 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6486

Symbol

SLC8B1

Alias : NCKX6, NCLX, SLC24A6

Description

solute carrier family 8 (sodium/lithium/calcium exchanger), member B1

Original Clone ID

oc96486

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1752 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 584 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31909.1 0 100.0 SLC8B1 CCDS81744.1 1.8e-150 90.4 SLC8B1 CCDS13140.1 7.2e-13 25.3 SLC24A3 CCDS45156.1 5.6e-12 24.9 SLC24A4 CCDS45155.2 5.9e-12 24.9 SLC24A4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004837 113 243 PF01699 Sodium/calcium exchanger membrane region IPR004837 431 570 PF01699 Sodium/calcium exchanger membrane region
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 6 LNLRWALSVLCVLLMAETVSGT 27 PRIMARY 22 2 81 LDYLEGIFCHFPPSLLPLAVTLY 103 SECONDARY 23 3 108 LYLFLILGVTAAKFFCPNLSAIS 130 PRIMARY 23 4 172 GALFGAGVLVTTVVAGGITILHP 194 PRIMARY 23 5 201 PFFRDIVFYMVAVFLTFLMLFR 222 PRIMARY 22 6 228 AWALGYLGLYVFYVVTVILCTWI 250 PRIMARY 23 7 324 WKALKVFKLPVEFLLLLTVPVV 345 SECONDARY 22 8 357 PLNCLHLVISPLVVVLTLQSGTY 379 PRIMARY 23 9 385 GGLVPVWVVVVIAGTALASVTFF 407 PRIMARY 23 10 418 HWLFAFLGFLTSALWINAAATEV 440 SECONDARY 23 11 447 LGVVFRLSNTVLGLTLLAWGNSI 469 SECONDARY 23 12 488 FSACFGGIIFNILVGVGLGCLLQ 510 PRIMARY 23 13 523 DGLLVWVLAGALGLSLVFSLVSV 545 PRIMARY 23 14 557 GFCLLLFYLNFLVVALLTEFGVI 579 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011537051 584 609841 100.0 100.0 NP_079235 584 609841 100.0 100.0 NP_001317395 528 609841 90.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6486 Download

>KIEE6486  1752 bp
ATGGCCGGCAGAAGGCTGAATCTGCGCTGGGCACTGAGTGTGCTTTGTGTGCTGCTAATG
GCGGAGACAGTGTCTGGGACTAGGGGCTCGTCTACAGGAGCTCACATTAGCCCCCAGTTT
CCAGCTTCAGGTGTGAACCAGACCCCCGTGGTAGACTGCCGCAAGGTGTGTGGCCTGAAT
GTCTCTGACCGCTGTGACTTCATCCGGACCAACCCTGACTGCCACAGTGATGGGGGGTAC
CTGGACTACCTGGAAGGCATCTTCTGCCACTTCCCTCCCAGCCTCCTCCCTCTGGCTGTC
ACTCTCTACGTTTCCTGGCTGCTCTACCTGTTTCTGATTCTGGGAGTCACCGCAGCCAAG
TTTTTCTGCCCCAACTTGTCGGCCATTTCTACCACACTGAAGCTCTCCCACAACGTGGCA
GGCGTCACCTTCCTGGCATTTGGGAATGGTGCACCTGACATCTTCAGTGCCCTGGTGGCC
TTCTCTGACCCGCACACAGCCGGCCTGGCCCTTGGGGCACTGTTTGGCGCTGGCGTGCTG
GTTACCACAGTGGTGGCCGGAGGCATTACCATCCTACACCCCTTCATGGCTGCCTCCAGG
CCCTTCTTCAGGGACATCGTTTTCTACATGGTGGCTGTGTTCCTGACCTTCCTCATGCTC
TTCCGTGGCAGGGTCACCCTGGCATGGGCTCTGGGTTACCTGGGCTTGTATGTGTTCTAT
GTGGTCACTGTGATTCTCTGCACCTGGATCTACCAACGGCAACGGAGAGGATCTCTGTTC
TGCCCCATGCCAGTTACTCCAGAGATCCTCTCAGACTCCGAGGAGGACCGGGTATCTTCT
AATACCAACAGCTATGACTACGGTGATGAGTACCGGCCGCTGTTCTTCTACCAGGAGACC
ACGGCTCAGATCCTGGTCCGGGCCCTCAATCCCCTGGATTACATGAAGTGGAGAAGGAAA
TCAGCATACTGGAAAGCCCTCAAGGTGTTCAAGCTGCCTGTGGAGTTCCTGCTGCTCCTC
ACAGTCCCCGTCGTGGACCCGGACAAGGATGACCAGAACTGGAAACGGCCCCTCAACTGT
CTGCATCTGGTTATCAGCCCCCTGGTTGTGGTCCTGACCCTGCAGTCGGGGACCTATGGT
GTCTATGAGATAGGCGGCCTCGTTCCCGTCTGGGTCGTGGTGGTGATCGCAGGCACAGCC
TTGGCTTCAGTGACCTTTTTTGCCACATCTGACAGCCAGCCCCCCAGGCTTCACTGGCTC
TTTGCTTTCCTGGGCTTTCTGACCAGCGCCCTGTGGATCAACGCGGCCGCCACAGAGGTG
GTGAACATCTTGCGGTCCCTGGGTGTGGTCTTCCGGCTGAGCAACACTGTGCTGGGGCTC
ACGCTGCTGGCCTGGGGGAACAGCATTGGAGATGCCTTCTCGGATTTCACACTGGCTCGC
CAGGGCTACCCACGGATGGCGTTCTCCGCCTGCTTTGGCGGCATCATCTTCAACATCCTC
GTGGGTGTGGGGCTGGGCTGCCTGCTCCAGATCTCCCGAAGCCACACAGAAGTGAAGCTG
GAGCCAGACGGACTGCTGGTGTGGGTCCTGGCAGGCGCCCTGGGGCTCAGCCTCGTCTTC
TCCCTGGTCTCAGTCCCATTGCAGTGCTTCCAGCTCAGCAGAGTCTATGGCTTCTGCCTG
CTCCTCTTCTACCTGAACTTCCTTGTCGTGGCCCTCCTCACTGAATTTGGAGTGATTCAC
CTGAAAAGCATG

ORF Protein

Cloned ORF protein sequence for pFN21AE6486 Download

>KIEE6486  584 aa
MAGRRLNLRWALSVLCVLLMAETVSGTRGSSTGAHISPQFPASGVNQTPVVDCRKVCGLN
VSDRCDFIRTNPDCHSDGGYLDYLEGIFCHFPPSLLPLAVTLYVSWLLYLFLILGVTAAK
FFCPNLSAISTTLKLSHNVAGVTFLAFGNGAPDIFSALVAFSDPHTAGLALGALFGAGVL
VTTVVAGGITILHPFMAASRPFFRDIVFYMVAVFLTFLMLFRGRVTLAWALGYLGLYVFY
VVTVILCTWIYQRQRRGSLFCPMPVTPEILSDSEEDRVSSNTNSYDYGDEYRPLFFYQET
TAQILVRALNPLDYMKWRRKSAYWKALKVFKLPVEFLLLLTVPVVDPDKDDQNWKRPLNC
LHLVISPLVVVLTLQSGTYGVYEIGGLVPVWVVVVIAGTALASVTFFATSDSQPPRLHWL
FAFLGFLTSALWINAAATEVVNILRSLGVVFRLSNTVLGLTLLAWGNSIGDAFSDFTLAR
QGYPRMAFSACFGGIIFNILVGVGLGCLLQISRSHTEVKLEPDGLLVWVLAGALGLSLVF
SLVSVPLQCFQLSRVYGFCLLLFYLNFLVVALLTEFGVIHLKSM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6486 Download

>pFN21AE6486  6457 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGCAGAAGGCTGAATCTGCGCTGGGCACT
GAGTGTGCTTTGTGTGCTGCTAATGGCGGAGACAGTGTCTGGGACTAGGGGCTCGTCTAC
AGGAGCTCACATTAGCCCCCAGTTTCCAGCTTCAGGTGTGAACCAGACCCCCGTGGTAGA
CTGCCGCAAGGTGTGTGGCCTGAATGTCTCTGACCGCTGTGACTTCATCCGGACCAACCC
TGACTGCCACAGTGATGGGGGGTACCTGGACTACCTGGAAGGCATCTTCTGCCACTTCCC
TCCCAGCCTCCTCCCTCTGGCTGTCACTCTCTACGTTTCCTGGCTGCTCTACCTGTTTCT
GATTCTGGGAGTCACCGCAGCCAAGTTTTTCTGCCCCAACTTGTCGGCCATTTCTACCAC
ACTGAAGCTCTCCCACAACGTGGCAGGCGTCACCTTCCTGGCATTTGGGAATGGTGCACC
TGACATCTTCAGTGCCCTGGTGGCCTTCTCTGACCCGCACACAGCCGGCCTGGCCCTTGG
GGCACTGTTTGGCGCTGGCGTGCTGGTTACCACAGTGGTGGCCGGAGGCATTACCATCCT
ACACCCCTTCATGGCTGCCTCCAGGCCCTTCTTCAGGGACATCGTTTTCTACATGGTGGC
TGTGTTCCTGACCTTCCTCATGCTCTTCCGTGGCAGGGTCACCCTGGCATGGGCTCTGGG
TTACCTGGGCTTGTATGTGTTCTATGTGGTCACTGTGATTCTCTGCACCTGGATCTACCA
ACGGCAACGGAGAGGATCTCTGTTCTGCCCCATGCCAGTTACTCCAGAGATCCTCTCAGA
CTCCGAGGAGGACCGGGTATCTTCTAATACCAACAGCTATGACTACGGTGATGAGTACCG
GCCGCTGTTCTTCTACCAGGAGACCACGGCTCAGATCCTGGTCCGGGCCCTCAATCCCCT
GGATTACATGAAGTGGAGAAGGAAATCAGCATACTGGAAAGCCCTCAAGGTGTTCAAGCT
GCCTGTGGAGTTCCTGCTGCTCCTCACAGTCCCCGTCGTGGACCCGGACAAGGATGACCA
GAACTGGAAACGGCCCCTCAACTGTCTGCATCTGGTTATCAGCCCCCTGGTTGTGGTCCT
GACCCTGCAGTCGGGGACCTATGGTGTCTATGAGATAGGCGGCCTCGTTCCCGTCTGGGT
CGTGGTGGTGATCGCAGGCACAGCCTTGGCTTCAGTGACCTTTTTTGCCACATCTGACAG
CCAGCCCCCCAGGCTTCACTGGCTCTTTGCTTTCCTGGGCTTTCTGACCAGCGCCCTGTG
GATCAACGCGGCCGCCACAGAGGTGGTGAACATCTTGCGGTCCCTGGGTGTGGTCTTCCG
GCTGAGCAACACTGTGCTGGGGCTCACGCTGCTGGCCTGGGGGAACAGCATTGGAGATGC
CTTCTCGGATTTCACACTGGCTCGCCAGGGCTACCCACGGATGGCGTTCTCCGCCTGCTT
TGGCGGCATCATCTTCAACATCCTCGTGGGTGTGGGGCTGGGCTGCCTGCTCCAGATCTC
CCGAAGCCACACAGAAGTGAAGCTGGAGCCAGACGGACTGCTGGTGTGGGTCCTGGCAGG
CGCCCTGGGGCTCAGCCTCGTCTTCTCCCTGGTCTCAGTCCCATTGCAGTGCTTCCAGCT
CAGCAGAGTCTATGGCTTCTGCCTGCTCCTCTTCTACCTGAACTTCCTTGTCGTGGCCCT
CCTCACTGAATTTGGAGTGATTCACCTGAAAAGCATGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT