Restriction map for
pFN21AE6486
(Circular) (Six-base) MAPSORT of: pFN21AE6486.seq Check: 3449 from: 1 to: 6457
pFN21AE6486 6457 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2096 2157 3800 1937
Size: 159 61 1643 4594
Fragments arranged by size:
4594 1643 159 61
Acc65I G'GTAC_C
Cuts at: 2241 3778 2241
Size: 1537 4920
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2423 2452 2596 3195 4520 5904 6172
Size: 483 308 29 144 599 1325 1384 268
Cuts at: 6172 1632
Size: 1917
Fragments arranged by size:
1917 1384 1325 599 483 308 268 144
29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 2555 3320 3554 1166
Size: 1389 765 234 4069
Fragments arranged by size:
4069 1389 765 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3389 1184
Size: 2205 4252
AlwNI CAG_nnn'CTG
Cuts at: 2078 2917 4200 5772 2078
Size: 839 1283 1572 2763
Fragments arranged by size:
2763 1572 1283 839
ApaI G_GGCC'C
Cuts at: 2929 2929
Size: 6457
ApaLI G'TGCA_C
Cuts at: 2454 4514 5670 2454
Size: 2060 1156 3241
Fragments arranged by size:
3241 2060 1156
ApoI r'AATT_y
Cuts at: 1493 3728 3766 3992 4144 6253 1493
Size: 2235 38 226 152 2109 1697
Fragments arranged by size:
2235 2109 1697 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 3782 1870
Size: 73 1839 4545
Fragments arranged by size:
4545 1839 73
BamHI G'GATC_C
Cuts at: 3787 4154 3787
Size: 367 6090
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2241 3599 3778 5233 618
Size: 325 217 627 454 1358 179 1455 1842
Fragments arranged by size:
1842 1455 1358 627 454 325 217 179
BanII G_rGCy'C
Cuts at: 1391 2093 2107 2695 2929 3384 3612 3776 1391
Size: 702 14 588 234 455 228 164 4072
Fragments arranged by size:
4072 702 588 455 234 228 164 14
BbeI G_GCGC'C
Cuts at: 3603 3603
Size: 6457
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3346 3613 928
Size: 971 1447 267 3772
Fragments arranged by size:
3772 1447 971 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4648 4682 1903
Size: 34 2711 34 3678
Fragments arranged by size:
3678 2711 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4349 5559 4349
Size: 1210 5247
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2480 2510 2519
Size: 107 122 71 631 605 808 30 9
Cuts at: 2519 3250 5068 136
Size: 731 1818 1525
Fragments arranged by size:
1818 1525 808 731 631 605 122 107
71 30 9
BglII A'GATC_T
Cuts at: 3534 6452 3534
Size: 2918 3539
BlpI GC'TnA_GC
Cuts at: 3362 3611 3659 3874 3362
Size: 249 48 215 5945
Fragments arranged by size:
5945 249 215 48
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2458 2476 2524 2929 4518 5674 1790
Size: 248 420 18 48 405 1589 1156 2573
Fragments arranged by size:
2573 1589 1156 420 405 248 48 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 4188 5146
Size: 416 53 83 399 24 696 2075 958
Cuts at: 5146 442
Size: 1753
Fragments arranged by size:
2075 1753 958 696 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2793 2830 2793
Size: 37 6420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2787 3515 3584 5117 1239
Size: 1345 203 728 69 1533 2579
Fragments arranged by size:
2579 1533 1345 728 203 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2969 4582 5447 5745 2969
Size: 1613 865 298 3681
Fragments arranged by size:
3681 1613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2894 3637 5120 882
Size: 617 1395 743 1483 2219
Fragments arranged by size:
2219 1483 1395 743 617
BsaAI yAC'GTr
Cuts at: 493 1204 4248 493
Size: 711 3044 2702
Fragments arranged by size:
3044 2702 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2427 3600 4264 4646
Size: 53 83 186 936 894 1173 664 382
Cuts at: 4646 5281 275
Size: 635 1451
Fragments arranged by size:
1451 1173 936 894 664 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2208 4890 5288 5562 5709 6120 2208
Size: 2682 398 274 147 411 2545
Fragments arranged by size:
2682 2545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 1178
Size: 30 165 30 657 30 5545
Fragments arranged by size:
5545 657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2840 3011 3677 3710 1186
Size: 1093 561 171 666 33 3933
Fragments arranged by size:
3933 1093 666 561 171 33
BseYI C'CCAG_C
Cuts at: 2031 2282 3376 3517 5660 6156 2031
Size: 251 1094 141 2143 496 2332
Fragments arranged by size:
2332 2143 1094 496 251 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2728 1163
Size: 573 992 4892
Fragments arranged by size:
4892 992 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2882 3312 3907 4668 5696 1540
Size: 275 186 881 430 595 761 1028 2301
Fragments arranged by size:
2301 1028 881 761 595 430 275 186
BsiHKAI G_wGCw'C
Cuts at: 2107 2458 4518 4603 5674 2107
Size: 351 2060 85 1071 2890
Fragments arranged by size:
2890 2060 1071 351 85
BsmI GAATG_Cn'
Cuts at: 3968 4061 3968
Size: 93 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2093 2107 2458 2476 2524 2695
Size: 399 248 55 14 351 18 48 171
Cuts at: 2695 2929 3384 3612 3776 4518 4603 5674 1391
Size: 234 455 228 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 399 351 248 234
228 171 164 85 55 48 18 14
BspEI T'CCGG_A
Cuts at: 2208 6120 2208
Size: 3912 2545
BspHI T'CATG_A
Cuts at: 4346 6076 4346
Size: 1730 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3812 844
Size: 2968 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 1394
Size: 429 2521 3507
Fragments arranged by size:
3507 2521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3641 4952 5126 65
Size: 1183 2393 1311 174 1396
Fragments arranged by size:
2393 1396 1311 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2504 2877 5101 5288 1700
Size: 110 199 495 373 2224 187 2869
Fragments arranged by size:
2869 2224 495 373 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 3503 4517 5529 3503
Size: 1014 1012 4431
Fragments arranged by size:
4431 1014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 794
Size: 1474 4983
BstBI TT'CG_AA
Cuts at: 5261 5261
Size: 6457
BstEII G'GTnAC_C
Cuts at: 1184 2545 2677 2699 1184
Size: 1361 132 22 4942
Fragments arranged by size:
4942 1361 132 22
BstXI CCAn_nnnn'nTGG
Cuts at: 2234 2557 3322 2234
Size: 323 765 5369
Fragments arranged by size:
5369 765 323
BstYI r'GATC_y
Cuts at: 1972 2751 2774 2808 2913 3534 3787 4154 4539
Size: 779 23 34 105 621 253 367 385
Cuts at: 4539 4556 6008 6094 6106 6452 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 779 621 385 367 346 253
105 86 34 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2668 2904 3455 513
Size: 552 939 147 517 236 551 3515
Fragments arranged by size:
3515 939 552 551 517 236 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 3653 3973 4767 4787 3653
Size: 320 794 20 5323
Fragments arranged by size:
5323 794 320 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2155 2190 529
Size: 35 1591 35 4796
Fragments arranged by size:
4796 1591 35 35
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DraIII CAC_nnn'GTG
Cuts at: 2042 2419 2042
Size: 377 6080
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3127 5464 809
Size: 507 1811 2337 1802
Fragments arranged by size:
2337 1811 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2385 2559
Size: 54 1425 50 275 18 177 378 174
Cuts at: 2559 2879 3309 3904 4793 6422 8
Size: 320 430 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 430 378 320 275
177 174 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2879 3309 3904 1537
Size: 275 1067 430 595 4090
Fragments arranged by size:
4090 1067 595 430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3695 4387 1353
Size: 1316 1026 692 3423
Fragments arranged by size:
3423 1316 1026 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 3460 5044 5418 5564 387
Size: 1064 138 492 1379 1584 374 146 1280
Fragments arranged by size:
1584 1379 1280 1064 492 374 146 138
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 6457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2423 2452 2584 2596 2787 3195
Size: 393 483 308 29 132 12 191 408
Cuts at: 3195 3515 3584 4520 5117 5904 6172 1239
Size: 320 69 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 483 408 393 320
308 268 191 132 69 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2611 2611
Size: 6457
EcoO109I rG'GnC_Cy
Cuts at: 2604 2926 3135 3589 2604
Size: 322 209 454 5472
Fragments arranged by size:
5472 454 322 209
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 2535 3294 3603 5604 1678
Size: 857 759 309 2001 2531
Fragments arranged by size:
2531 2001 857 759 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2822 2854 2893
Size: 32 135 32 488 32 730 32 39
Cuts at: 2893 2925 3407 3439 5098 5130 5172 5204 1373
Size: 32 482 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 730 488 482 135 42 39
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4047 669
Size: 988 281 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KasI G'GCGC_C
Cuts at: 3599 3599
Size: 6457
KpnI G_GTAC'C
Cuts at: 2245 3782 2245
Size: 1537 4920
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5571 5755 1305
Size: 4266 184 2007
Fragments arranged by size:
4266 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4635
Fragments arranged by size:
4635 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 2555 3320 3503 3554 3646 4415
Size: 648 1066 323 765 183 51 92 769
Cuts at: 4415 4774 4933 518
Size: 359 159 2042
Fragments arranged by size:
2042 1066 769 765 648 359 323 183
159 92 51
MspA1I CmG'CkG
Cuts at: 1606 2196 2884 3873 4550 5698 5943 1606
Size: 590 688 989 677 1148 245 2120
Fragments arranged by size:
2120 1148 989 688 677 590 245
NaeI GCC'GGC
Cuts at: 2011 2506 2011
Size: 495 5962
NarI GG'CG_CC
Cuts at: 3600 3600
Size: 6457
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4966
Fragments arranged by size:
4966 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NgoMIV G'CCGG_C
Cuts at: 2009 2504 2009
Size: 495 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3309 3904 3309
Size: 595 5862
NspI r_CATG'y
Cuts at: 1132 1229 3815 5360 1132
Size: 97 2586 1545 2229
Fragments arranged by size:
2586 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3343 3604 1348
Size: 1995 261 4201
Fragments arranged by size:
4201 1995 261
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 1108
Size: 1311 5146
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3135 3589 3135
Size: 454 6003
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6457
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PspOMI G'GGCC_C
Cuts at: 2925 2925
Size: 6457
PstI C_TGCA'G
Cuts at: 830 3129 3809 830
Size: 2299 680 3478
Fragments arranged by size:
3478 2299 680
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SfcI C'TryA_G
Cuts at: 826 1045 2097 3125 3805 4940 5621 5812 826
Size: 219 1052 1028 680 1135 681 191 1471
Fragments arranged by size:
1471 1135 1052 1028 681 680 219 191
SfoI GGC'GCC
Cuts at: 3601 3601
Size: 6457
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 3784 1872
Size: 1912 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2984 4561 5462 5724 820
Size: 197 926 1041 1577 901 262 1553
Fragments arranged by size:
1577 1553 1041 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6457
StyI C'CwwG_G
Cuts at: 513 1065 2004 2515 3204 3896 513
Size: 552 939 511 689 692 3074
Fragments arranged by size:
3074 939 692 689 552 511
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 3497 4484 4501 4654 4839 5284
Size: 1255 1755 162 987 17 153 185 445
Cuts at: 5284 325
Size: 1498
Fragments arranged by size:
1755 1498 1255 987 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4703 6415 96
Size: 274 80 33 51 1443 2726 1712 138
Fragments arranged by size:
2726 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3127 3159 3567 3599 6417 6449 3127
Size: 32 408 32 2818 32 3135
Fragments arranged by size:
3135 2818 408 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2074 1316
Size: 758 5699
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 3782 1870
Size: 1912 4545
XmnI GAAnn'nnTTC
Cuts at: 4586 4586
Size: 6457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI
XcmI