(Linear) (Six-base) MAPSORT of: KIEE6486.seq Check: 8394 from: 1 to: 1752 KIEE6486.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 91 152 1752 Size: 91 61 1600 Fragments arranged by size: 1600 91 61 Acc65I G'GTAC_C Cuts at: 0 236 1752 Size: 236 1516 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 418 447 591 1190 1752 Size: 110 308 29 144 599 562 Fragments arranged by size: 599 562 308 144 110 29 AleI CACnn'nnGTG Cuts at: 0 550 1315 1549 1752 Size: 550 765 234 203 Fragments arranged by size: 765 550 234 203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1384 1752 Size: 1384 368 AlwNI CAG_nnn'CTG Cuts at: 0 73 912 1752 Size: 73 839 840 Fragments arranged by size: 840 839 73 ApaI G_GGCC'C Cuts at: 0 924 1752 Size: 924 828 ApaLI G'TGCA_C Cuts at: 0 449 1752 Size: 449 1303 ApoI r'AATT_y Cuts at: 0 1723 1752 Size: 1723 29 BanI G'GyrC_C Cuts at: 0 236 1594 1752 Size: 236 1358 158 Fragments arranged by size: 1358 236 158 BanII G_rGCy'C Cuts at: 0 88 102 690 924 1379 1607 1752 Size: 88 14 588 234 455 228 145 Fragments arranged by size: 588 455 234 228 145 88 14 BbeI G_GCGC'C Cuts at: 0 1598 1752 Size: 1598 154 BbsI GAAGACnn'nnnn_ Cuts at: 0 1341 1608 1752 Size: 1341 267 144 Fragments arranged by size: 1341 267 144 BglI GCCn_nnn'nGGC Cuts at: 0 475 505 514 1245 1752 Size: 475 30 9 731 507 Fragments arranged by size: 731 507 475 30 9 BglII A'GATC_T Cuts at: 0 1529 1752 Size: 1529 223 BlpI GC'TnA_GC Cuts at: 0 1357 1606 1654 1752 Size: 1357 249 48 98 Fragments arranged by size: 1357 249 98 48 Bme1580I G_kGCm'C Cuts at: 0 33 453 471 519 924 1752 Size: 33 420 18 48 405 828 Fragments arranged by size: 828 420 405 48 33 18 BmrI ACTGGGnnnn_n' Cuts at: 0 108 1752 Size: 108 1644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 788 825 1752 Size: 788 37 927 Fragments arranged by size: 927 788 37 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 579 782 1510 1579 1752 Size: 579 203 728 69 173 Fragments arranged by size: 728 579 203 173 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 1752 Size: 964 788 BsaI GGTCTCn'nnnn_ Cuts at: 0 889 1632 1752 Size: 889 743 120 Fragments arranged by size: 889 743 120 BsaHI Gr'CG_yC Cuts at: 0 422 1595 1752 Size: 422 1173 157 Fragments arranged by size: 1173 422 157 BsaWI w'CCGG_w Cuts at: 0 203 1752 Size: 203 1549 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 55 85 1752 Size: 55 30 1667 Fragments arranged by size: 1667 55 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 274 835 1006 1672 1705 1752 Size: 274 561 171 666 33 47 Fragments arranged by size: 666 561 274 171 47 33 BseYI C'CCAG_C Cuts at: 0 26 277 1371 1512 1752 Size: 26 251 1094 141 240 Fragments arranged by size: 1094 251 240 141 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 723 1752 Size: 723 1029 BsiEI CG_ry'CG Cuts at: 0 877 1307 1752 Size: 877 430 445 Fragments arranged by size: 877 445 430 BsiHKAI G_wGCw'C Cuts at: 0 102 453 1752 Size: 102 351 1299 Fragments arranged by size: 1299 351 102 Bsp1286I G_dGCh'C Cuts at: 0 33 88 102 453 471 519 690 924 Size: 33 55 14 351 18 48 171 234 Cuts at: 924 1379 1607 1752 Size: 455 228 145 Fragments arranged by size: 455 351 234 228 171 145 55 48 33 18 14 BspEI T'CCGG_A Cuts at: 0 203 1752 Size: 203 1549 BsrDI GCAATG_nn' Cuts at: 0 1636 1752 Size: 1636 116 BsrFI r'CCGG_y Cuts at: 0 4 499 872 1752 Size: 4 495 373 880 Fragments arranged by size: 880 495 373 4 BssSI C'ACGA_G Cuts at: 0 1498 1752 Size: 1498 254 BstAPI GCAn_nnn'nTGC Cuts at: 0 263 1752 Size: 263 1489 BstEII G'GTnAC_C Cuts at: 0 540 672 694 1752 Size: 540 132 22 1058 Fragments arranged by size: 1058 540 132 22 BstXI CCAn_nnnn'nTGG Cuts at: 0 229 552 1317 1752 Size: 229 323 765 435 Fragments arranged by size: 765 435 323 229 BstYI r'GATC_y Cuts at: 0 746 769 803 908 1529 1752 Size: 746 23 34 105 621 223 Fragments arranged by size: 746 621 223 105 34 23 BtgI C'CryG_G Cuts at: 0 146 663 899 1450 1752 Size: 146 517 236 551 302 Fragments arranged by size: 551 517 302 236 146 BtsI GCAGTG_nn' Cuts at: 0 1648 1752 Size: 1648 104 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 150 185 1752 Size: 150 35 1567 Fragments arranged by size: 1567 150 35 DraIII CAC_nnn'GTG Cuts at: 0 37 414 1752 Size: 37 377 1338 Fragments arranged by size: 1338 377 37 DrdI GACnn_nn'nnGTC Cuts at: 0 1122 1752 Size: 1122 630 EaeI y'GGCC_r Cuts at: 0 2 380 554 874 1304 1752 Size: 2 378 174 320 430 448 Fragments arranged by size: 448 430 378 320 174 2 EagI C'GGCC_G Cuts at: 0 874 1304 1752 Size: 874 430 448 Fragments arranged by size: 874 448 430 EarI CTCTTCn'nnn_ Cuts at: 0 664 1690 1752 Size: 664 1026 62 Fragments arranged by size: 1026 664 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 76 1455 1752 Size: 76 1379 297 Fragments arranged by size: 1379 297 76 EcoICRI GAG'CTC Cuts at: 0 100 1752 Size: 100 1652 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 418 447 579 591 782 1190 1510 Size: 110 308 29 132 12 191 408 320 Cuts at: 1510 1579 1752 Size: 69 173 Fragments arranged by size: 408 320 308 191 173 132 110 69 29 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 606 1752 Size: 606 1146 EcoO109I rG'GnC_Cy Cuts at: 0 599 921 1130 1584 1752 Size: 599 322 209 454 168 Fragments arranged by size: 599 454 322 209 168 HaeII r_GCGC'y Cuts at: 0 530 1289 1598 1752 Size: 530 759 309 154 Fragments arranged by size: 759 530 309 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 817 849 888 920 1402 1434 Size: 55 32 730 32 39 32 482 32 Cuts at: 1434 1752 Size: 318 Fragments arranged by size: 730 482 318 55 39 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1594 1752 Size: 1594 158 KpnI G_GTAC'C Cuts at: 0 240 1752 Size: 240 1512 MslI CAynn'nnrTG Cuts at: 0 227 550 1315 1498 1549 1641 1752 Size: 227 323 765 183 51 92 111 Fragments arranged by size: 765 323 227 183 111 92 51 MspA1I CmG'CkG Cuts at: 0 191 879 1752 Size: 191 688 873 Fragments arranged by size: 873 688 191 NaeI GCC'GGC Cuts at: 0 6 501 1752 Size: 6 495 1251 Fragments arranged by size: 1251 495 6 NarI GG'CG_CC Cuts at: 0 1595 1752 Size: 1595 157 NgoMIV G'CCGG_C Cuts at: 0 4 499 1752 Size: 4 495 1253 Fragments arranged by size: 1253 495 4 NotI GC'GGCC_GC Cuts at: 0 1304 1752 Size: 1304 448 PasI CC'CwG_GG Cuts at: 0 1338 1599 1752 Size: 1338 261 153 Fragments arranged by size: 1338 261 153 PflMI CCAn_nnn'nTGG Cuts at: 0 414 1752 Size: 414 1338 PpuMI rG'GwC_Cy Cuts at: 0 1130 1584 1752 Size: 1130 454 168 Fragments arranged by size: 1130 454 168 PspOMI G'GGCC_C Cuts at: 0 920 1752 Size: 920 832 PstI C_TGCA'G Cuts at: 0 1124 1752 Size: 1124 628 SacI G_AGCT'C Cuts at: 0 102 1752 Size: 102 1650 SapI GCTCTTCn'nnn_ Cuts at: 0 664 1752 Size: 664 1088 SfcI C'TryA_G Cuts at: 0 92 1120 1752 Size: 92 1028 632 Fragments arranged by size: 1028 632 92 SfoI GGC'GCC Cuts at: 0 1596 1752 Size: 1596 156 SmlI C'TyrA_G Cuts at: 0 979 1752 Size: 979 773 StyI C'CwwG_G Cuts at: 0 510 1199 1752 Size: 510 689 553 Fragments arranged by size: 689 553 510 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1330 1492 1752 Size: 1330 162 260 Fragments arranged by size: 1330 260 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1122 1154 1562 1594 1752 Size: 1122 32 408 32 158 Fragments arranged by size: 1122 408 158 32 32 Tth111I GACn'n_nGTC Cuts at: 0 69 1752 Size: 69 1683 Enzymes that do cut: AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApaLI ApoI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PpuMI PspOMI PstI SacI SapI SfcI SfoI SmlI StyI TaqII TstI Tth111I Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI DraI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmaI XmnI ZraI