RHCG ORF clone pFN21AE6476

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Halotag ORF Clone pFN21AE6476

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Clone info.

Species

human

Clone Name

pFN21AE6476 Sequence (with vector) Restriction Map

Product ID

FHC25500

ORF Sequence

DNA sequence (1437 bp)
Protein sequence (479 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6476

Symbol

RHCG

Alias : C15orf6, PDRC2, RHGK, SLC42A3

Description

Rh family, C glycoprotein, transcript variant 1

Original Clone ID

oc96476

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1437 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 479 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10351.1 6e-196 100.0 RHCG CCDS41414.2 8.2e-102 57.4 RHBG CCDS4927.1 1.1e-78 50.7 RHAG CCDS60030.1 1.6e-32 31.6 RHD CCDS262.1 2e-32 31.7 RHD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002229 10 28 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 60 77 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 89 106 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 127 143 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 150 173 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 184 199 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 219 237 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 249 271 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 284 297 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 307 325 PR00342 Blood group Rhesus C/E/D polypeptide IPR002229 345 361 PR00342 Blood group Rhesus C/E/D polypeptide HMMPfam IPR024041 48 418 PF00909 Ammonium transporter AmtB-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 11 LPLTCLLLQVIMVILFGVFVRY 32 PRIMARY 22 2 61 PSFQDVHVMVFVGFGFLMTFLQR 83 SECONDARY 23 3 85 GFSAVGFNFLLAAFGIQWALLMQ 107 PRIMARY 23 4 121 GVENLINADFCVASVCVAFGAVL 143 PRIMARY 23 5 151 LLIMTFFQVTLFAVNEFILLNLL 173 PRIMARY 23 6 215 YQSDLFAMIGTLFLWMYWPSFNS 237 SECONDARY 23 7 250 AINTYCSLAACVLTSVAISSALH 272 SECONDARY 23 8 301 LMPYGALIIGFVCGIISTLGFVY 323 SECONDARY 23 9 343 LHGIPGIIGGIVGAVTAASASL 364 SECONDARY 22 10 396 YGLLVTLAMALMGGIIVGLILRL 418 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057405 479 605381 100.0 100.0 NP_001307970 479 605381 100.0 100.0 NP_065140 458 607079 57.4 90.2 XP_016857346 428 607079 58.2 85.8 XP_016857347 414 607079 58.1 85.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6476 Download

>KIEE6476  1437 bp
ATGGCCTGGAACACCAACCTCCGCTGGCGGCTGCCGCTCACCTGCCTGCTCCTGCAGGTG
ATTATGGTGATTCTCTTCGGGGTGTTCGTGCGCTACGACTTCGAGGCCGACGCCCACTGG
TGGTCAGAGAGGACGCACAAGAACTTGAGCGACATGGAGAACGAATTCTACTATCGCTAC
CCAAGCTTCCAGGACGTGCACGTGATGGTCTTCGTGGGCTTCGGCTTCCTCATGACTTTC
CTGCAGCGCTACGGCTTCAGCGCCGTGGGCTTCAACTTCCTGTTGGCAGCCTTCGGCATC
CAGTGGGCGCTGCTCATGCAGGGCTGGTTCCACTTCTTACAAGACCGCTACATCGTCGTG
GGCGTGGAGAACCTCATCAACGCTGACTTCTGCGTGGCCTCTGTCTGCGTGGCCTTTGGG
GCAGTTCTGGGTAAAGTCAGCCCCATTCAGCTGCTCATCATGACTTTCTTCCAAGTGACC
CTCTTCGCTGTGAATGAGTTCATTCTCCTTAACCTGCTAAAGGTGAAGGATGCAGGAGGC
TCCATGACCATCCACACATTTGGCGCCTACTTTGGGCTCACAGTGACCCGGATCCTCTAC
CGACGCAACCTAGAGCAGAGCAAGGAGAGACAGAATTCTGTGTACCAGTCGGACCTCTTT
GCCATGATTGGCACCCTCTTCCTGTGGATGTACTGGCCCAGCTTCAACTCAGCCATATCC
TACCATGGGGACAGCCAGCACCGAGCCGCCATCAACACCTACTGCTCCTTGGCAGCCTGC
GTGCTTACCTCGGTGGCAATATCCAGTGCCCTGCACAAGAAGGGCAAGCTGGACATGGTG
CACATCCAGAATGCCACGCTCGCAGGAGGGGTGGCCGTGGGTACCGCTGCTGAGATGATG
CTCATGCCTTACGGTGCCCTCATCATCGGCTTCGTCTGCGGCATCATCTCCACCCTGGGT
TTTGTATACCTGACCCCATTCCTGGAGTCCCGGCTGCACATCCAGGACACATGCGGCATT
AACAATCTGCATGGCATTCCTGGCATCATAGGCGGCATCGTGGGTGCTGTGACAGCGGCC
TCCGCCAGCCTTGAAGTCTATGGAAAAGAAGGGCTTGTCCATTCCTTTGACTTTCAAGGT
TTCAACGGGGACTGGACCGCAAGAACACAGGGAAAGTTCCAGATTTATGGTCTCTTGGTG
ACCCTGGCCATGGCCCTGATGGGTGGCATCATTGTGGGGCTCATTTTGAGATTACCATTC
TGGGGACAACCTTCAGATGAGAACTGCTTTGAGGATGCGGTCTACTGGGAGATGCCTGAA
GGGAACAGCACTGTCTACATCCCTGAGGACCCCACCTTCAAGCCCTCAGGACCCTCAGTA
CCCTCAGTACCCATGGTGTCCCCACTACCCATGGCTTCCTCGGTACCCTTGGTACCC

ORF Protein

Cloned ORF protein sequence for pFN21AE6476 Download

>KIEE6476  479 aa
MAWNTNLRWRLPLTCLLLQVIMVILFGVFVRYDFEADAHWWSERTHKNLSDMENEFYYRY
PSFQDVHVMVFVGFGFLMTFLQRYGFSAVGFNFLLAAFGIQWALLMQGWFHFLQDRYIVV
GVENLINADFCVASVCVAFGAVLGKVSPIQLLIMTFFQVTLFAVNEFILLNLLKVKDAGG
SMTIHTFGAYFGLTVTRILYRRNLEQSKERQNSVYQSDLFAMIGTLFLWMYWPSFNSAIS
YHGDSQHRAAINTYCSLAACVLTSVAISSALHKKGKLDMVHIQNATLAGGVAVGTAAEMM
LMPYGALIIGFVCGIISTLGFVYLTPFLESRLHIQDTCGINNLHGIPGIIGGIVGAVTAA
SASLEVYGKEGLVHSFDFQGFNGDWTARTQGKFQIYGLLVTLAMALMGGIIVGLILRLPF
WGQPSDENCFEDAVYWEMPEGNSTVYIPEDPTFKPSGPSVPSVPMVSPLPMASSVPLVP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6476 Download

>pFN21AE6476  6142 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCTGGAACACCAACCTCCGCTGGCGGCTGCC
GCTCACCTGCCTGCTCCTGCAGGTGATTATGGTGATTCTCTTCGGGGTGTTCGTGCGCTA
CGACTTCGAGGCCGACGCCCACTGGTGGTCAGAGAGGACGCACAAGAACTTGAGCGACAT
GGAGAACGAATTCTACTATCGCTACCCAAGCTTCCAGGACGTGCACGTGATGGTCTTCGT
GGGCTTCGGCTTCCTCATGACTTTCCTGCAGCGCTACGGCTTCAGCGCCGTGGGCTTCAA
CTTCCTGTTGGCAGCCTTCGGCATCCAGTGGGCGCTGCTCATGCAGGGCTGGTTCCACTT
CTTACAAGACCGCTACATCGTCGTGGGCGTGGAGAACCTCATCAACGCTGACTTCTGCGT
GGCCTCTGTCTGCGTGGCCTTTGGGGCAGTTCTGGGTAAAGTCAGCCCCATTCAGCTGCT
CATCATGACTTTCTTCCAAGTGACCCTCTTCGCTGTGAATGAGTTCATTCTCCTTAACCT
GCTAAAGGTGAAGGATGCAGGAGGCTCCATGACCATCCACACATTTGGCGCCTACTTTGG
GCTCACAGTGACCCGGATCCTCTACCGACGCAACCTAGAGCAGAGCAAGGAGAGACAGAA
TTCTGTGTACCAGTCGGACCTCTTTGCCATGATTGGCACCCTCTTCCTGTGGATGTACTG
GCCCAGCTTCAACTCAGCCATATCCTACCATGGGGACAGCCAGCACCGAGCCGCCATCAA
CACCTACTGCTCCTTGGCAGCCTGCGTGCTTACCTCGGTGGCAATATCCAGTGCCCTGCA
CAAGAAGGGCAAGCTGGACATGGTGCACATCCAGAATGCCACGCTCGCAGGAGGGGTGGC
CGTGGGTACCGCTGCTGAGATGATGCTCATGCCTTACGGTGCCCTCATCATCGGCTTCGT
CTGCGGCATCATCTCCACCCTGGGTTTTGTATACCTGACCCCATTCCTGGAGTCCCGGCT
GCACATCCAGGACACATGCGGCATTAACAATCTGCATGGCATTCCTGGCATCATAGGCGG
CATCGTGGGTGCTGTGACAGCGGCCTCCGCCAGCCTTGAAGTCTATGGAAAAGAAGGGCT
TGTCCATTCCTTTGACTTTCAAGGTTTCAACGGGGACTGGACCGCAAGAACACAGGGAAA
GTTCCAGATTTATGGTCTCTTGGTGACCCTGGCCATGGCCCTGATGGGTGGCATCATTGT
GGGGCTCATTTTGAGATTACCATTCTGGGGACAACCTTCAGATGAGAACTGCTTTGAGGA
TGCGGTCTACTGGGAGATGCCTGAAGGGAACAGCACTGTCTACATCCCTGAGGACCCCAC
CTTCAAGCCCTCAGGACCCTCAGTACCCTCAGTACCCATGGTGTCCCCACTACCCATGGC
TTCCTCGGTACCCTTGGTACCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT