Restriction map for
pFN21AE6476
(Linear) (Six-base) MAPSORT of: KIEE6476.seq Check: 3971 from: 1 to: 1437
KIEE6476.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 49 1437
Size: 45 4 1388
Fragments arranged by size:
1388 45 4
AccI GT'mk_AC
Cuts at: 0 965 1301 1334 1437
Size: 965 336 33 103
Fragments arranged by size:
965 336 103 33
Acc65I G'GTAC_C
Cuts at: 0 880 1422 1431 1437
Size: 880 542 9 6
Fragments arranged by size:
880 542 9 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1256 1337 1437
Size: 240 1016 81 100
Fragments arranged by size:
1016 240 100 81
AfeI AGC'GCT
Cuts at: 0 247 1437
Size: 247 1190
ApaLI G'TGCA_C
Cuts at: 0 196 838 1437
Size: 196 642 599
Fragments arranged by size:
642 599 196
ApoI r'AATT_y
Cuts at: 0 163 633 1437
Size: 163 470 804
Fragments arranged by size:
804 470 163
BamHI G'GATC_C
Cuts at: 0 590 1437
Size: 590 847
BanI G'GyrC_C
Cuts at: 0 562 670 880 913 1422 1431 1437
Size: 562 108 210 33 509 9 6
Fragments arranged by size:
562 509 210 108 33 9 6
BanII G_rGCy'C
Cuts at: 0 578 1241 1437
Size: 578 663 196
Fragments arranged by size:
663 578 196
BbeI G_GCGC'C
Cuts at: 0 566 1437
Size: 566 871
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 1437
Size: 201 1236
BglI GCCn_nnn'nGGC
Cuts at: 0 418 1437
Size: 418 1019
Bme1580I G_kGCm'C
Cuts at: 0 200 810 842 918 1437
Size: 200 610 32 76 519
Fragments arranged by size:
610 519 200 76 32
BmgBI CAC'GTC
Cuts at: 0 195 1437
Size: 195 1242
BmrI ACTGGGnnnn_n'
Cuts at: 0 1314 1437
Size: 1314 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1437
Size: 1003 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1437
Size: 165 1272
BsaI GGTCTCn'nnnn_
Cuts at: 0 1195 1437
Size: 1195 242
BsaAI yAC'GTr
Cuts at: 0 201 1437
Size: 201 1236
BsaHI Gr'CG_yC
Cuts at: 0 110 563 1437
Size: 110 453 874
Fragments arranged by size:
874 453 110
BseYI C'CCAG_C
Cuts at: 0 697 1437
Size: 697 740
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 979 1437
Size: 796 183 458
Fragments arranged by size:
796 458 183
BsiHKAI G_wGCw'C
Cuts at: 0 200 842 1437
Size: 200 642 595
Fragments arranged by size:
642 595 200
BsmI GAATG_Cn'
Cuts at: 0 855 1034 1437
Size: 855 179 403
Fragments arranged by size:
855 403 179
Bsp1286I G_dGCh'C
Cuts at: 0 200 578 810 842 918 1241 1437
Size: 200 378 232 32 76 323 196
Fragments arranged by size:
378 323 232 200 196 76 32
BspHI T'CATG_A
Cuts at: 0 230 458 1437
Size: 230 228 979
Fragments arranged by size:
979 230 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 49 521 1437
Size: 45 4 472 916
Fragments arranged by size:
916 472 45 4
BsrBI CCG'CTC
Cuts at: 0 36 1437
Size: 36 1401
BstEII G'GTnAC_C
Cuts at: 0 1197 1437
Size: 1197 240
BstXI CCAn_nnnn'nTGG
Cuts at: 0 559 1186 1437
Size: 559 627 251
Fragments arranged by size:
627 559 251
BstYI r'GATC_y
Cuts at: 0 590 1437
Size: 590 847
BstZ17I GTA'TAC
Cuts at: 0 966 1437
Size: 966 471
Bsu36I CC'TnA_GG
Cuts at: 0 1343 1365 1437
Size: 1343 22 72
Fragments arranged by size:
1343 72 22
BtgI C'CryG_G
Cuts at: 0 263 723 875 1208 1391 1409 1437
Size: 263 460 152 333 183 18 28
Fragments arranged by size:
460 333 263 183 152 28 18
EaeI y'GGCC_r
Cuts at: 0 872 1205 1437
Size: 872 333 232
Fragments arranged by size:
872 333 232
EarI CTCTTCn'nnn_
Cuts at: 0 79 487 682 1437
Size: 79 408 195 755
Fragments arranged by size:
755 408 195 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1071 1437
Size: 1071 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1003 1256 1337 1437
Size: 240 763 253 81 100
Fragments arranged by size:
763 253 240 100 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 517 1437
Size: 517 920
EcoO109I rG'GnC_Cy
Cuts at: 0 1347 1369 1437
Size: 1347 22 68
Fragments arranged by size:
1347 68 22
EcoRI G'AATT_C
Cuts at: 0 163 633 1437
Size: 163 470 804
Fragments arranged by size:
804 470 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1129 1437
Size: 1129 308
HaeII r_GCGC'y
Cuts at: 0 249 263 310 566 1437
Size: 249 14 47 256 871
Fragments arranged by size:
871 256 249 47 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 734 766 1173 1205 1437
Size: 734 32 407 32 232
Fragments arranged by size:
734 407 232 32 32
HindIII A'AGCT_T
Cuts at: 0 183 1437
Size: 183 1254
KasI G'GCGC_C
Cuts at: 0 562 1437
Size: 562 875
KpnI G_GTAC'C
Cuts at: 0 884 1426 1435 1437
Size: 884 542 9 2
Fragments arranged by size:
884 542 9 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1437
Size: 629 808
MscI TGG'CCA
Cuts at: 0 1207 1437
Size: 1207 230
MslI CAynn'nnrTG
Cuts at: 0 355 1231 1437
Size: 355 876 206
Fragments arranged by size:
876 355 206
MspA1I CmG'CkG
Cuts at: 0 23 450 886 1075 1437
Size: 23 427 436 189 362
Fragments arranged by size:
436 427 362 189 23
NarI GG'CG_CC
Cuts at: 0 563 1437
Size: 563 874
NcoI C'CATG_G
Cuts at: 0 723 1208 1391 1409 1437
Size: 723 485 183 18 28
Fragments arranged by size:
723 485 183 28 18
NspI r_CATG'y
Cuts at: 0 1013 1437
Size: 1013 424
PasI CC'CwG_GG
Cuts at: 0 954 1437
Size: 954 483
PfoI T'CCnGG_A
Cuts at: 0 188 980 1001 1437
Size: 188 792 21 436
Fragments arranged by size:
792 436 188 21
PmlI CAC'GTG
Cuts at: 0 201 1437
Size: 201 1236
PpuMI rG'GwC_Cy
Cuts at: 0 1347 1369 1437
Size: 1347 22 68
Fragments arranged by size:
1347 68 22
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 151 183 1437
Size: 151 32 1254
Fragments arranged by size:
1254 151 32
PstI C_TGCA'G
Cuts at: 0 56 245 1437
Size: 56 189 1192
Fragments arranged by size:
1192 189 56
PvuII CAG'CTG
Cuts at: 0 450 1437
Size: 450 987
SbfI CC_TGCA'GG
Cuts at: 0 56 1437
Size: 56 1381
SfcI C'TryA_G
Cuts at: 0 52 241 1437
Size: 52 189 1196
Fragments arranged by size:
1196 189 52
SfoI GGC'GCC
Cuts at: 0 564 1437
Size: 564 873
SmlI C'TyrA_G
Cuts at: 0 144 1437
Size: 144 1293
StyI C'CwwG_G
Cuts at: 0 723 767 1208 1391 1409 1427 1437
Size: 723 44 441 183 18 18 10
Fragments arranged by size:
723 441 183 44 18 18 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1051 1210 1437
Size: 1051 159 227
Fragments arranged by size:
1051 227 159
TatI w'GTAC_w
Cuts at: 0 689 1437
Size: 689 748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1215 1437
Size: 1215 222
XmnI GAAnn'nnTTC
Cuts at: 0 163 496 1437
Size: 163 333 941
Fragments arranged by size:
941 333 163
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI ApaLI ApoI BamHI
BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaHI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PfoI PmlI PpuMI PsrI PstI PvuII SbfI SfcI
SfoI SmlI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI Bpu10I BsaBI BsaWI BsaXI BseRI BsiEI
BsiWI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRV FseI FspI FspAI HincII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI