(Circular) (Six-base) MAPSORT of: pFN21AE6476.seq Check: 447 from: 1 to: 6142 pFN21AE6476 6142 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2054 2050 Size: 4 6138 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2970 3306 3339 3485 1937 Size: 1033 336 33 146 4594 Fragments arranged by size: 4594 1033 336 146 33 Acc65I G'GTAC_C Cuts at: 2885 3427 3436 3463 2885 Size: 542 9 27 5564 Fragments arranged by size: 5564 542 27 9 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2245 3261 3342 4205 5589 5857 1632 Size: 613 1016 81 863 1384 268 1917 Fragments arranged by size: 1917 1384 1016 863 613 268 81 AfeI AGC'GCT Cuts at: 2252 2252 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 3885 5457 3885 Size: 1572 4570 ApaLI G'TGCA_C Cuts at: 2201 2843 4199 5355 2201 Size: 642 1356 1156 2988 Fragments arranged by size: 2988 1356 1156 642 ApoI r'AATT_y Cuts at: 1493 2168 2638 3451 3677 3829 5938 1493 Size: 675 470 813 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 675 470 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3467 1870 Size: 73 1524 4545 Fragments arranged by size: 4545 1524 73 BamHI G'GATC_C Cuts at: 2595 3472 3839 2595 Size: 877 367 4898 Fragments arranged by size: 4898 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 2675 2885 2918 3427 Size: 325 217 627 780 108 210 33 509 Cuts at: 3427 3436 3463 4918 618 Size: 9 27 1455 1842 Fragments arranged by size: 1842 1455 780 627 509 325 217 210 108 33 27 9 BanII G_rGCy'C Cuts at: 1391 2583 3246 3461 1391 Size: 1192 663 215 4072 Fragments arranged by size: 4072 1192 663 215 BbeI G_GCGC'C Cuts at: 2571 2571 Size: 6142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 928 Size: 971 307 4864 Fragments arranged by size: 4864 971 307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 4753 136 Size: 107 122 71 631 605 751 2330 1525 Fragments arranged by size: 2330 1525 751 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2205 2815 2847 2923 4203 5359 1790 Size: 415 610 32 76 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 610 415 76 32 BmgBI CAC'GTC Cuts at: 1318 2200 1318 Size: 882 5260 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3319 3873 4831 Size: 416 53 83 399 24 1902 554 958 Cuts at: 4831 442 Size: 1753 Fragments arranged by size: 1902 1753 958 554 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3008 4802 1239 Size: 1769 1794 2579 Fragments arranged by size: 2579 1794 1769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2170 4267 5132 5430 2170 Size: 2097 865 298 2882 Fragments arranged by size: 2882 2097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3200 4805 882 Size: 617 1701 1605 2219 Fragments arranged by size: 2219 1701 1605 617 BsaAI yAC'GTr Cuts at: 493 1204 2206 3933 493 Size: 711 1002 1727 2702 Fragments arranged by size: 2702 1727 1002 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2568 3949 4331 Size: 53 83 186 936 582 453 1381 382 Cuts at: 4331 4966 275 Size: 635 1451 Fragments arranged by size: 1451 1381 936 635 582 453 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5917 Fragments arranged by size: 5917 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6142 BseYI C'CCAG_C Cuts at: 2702 5345 5841 2702 Size: 2643 496 3003 Fragments arranged by size: 3003 2643 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 2984 1163 Size: 573 1065 183 4321 Fragments arranged by size: 4321 1065 573 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 2847 4203 4288 5359 2205 Size: 642 1356 85 1071 2988 Fragments arranged by size: 2988 1356 1071 642 85 BsmI GAATG_Cn' Cuts at: 2860 3039 3653 3746 2860 Size: 179 614 93 5256 Fragments arranged by size: 5256 614 179 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2583 2815 2847 2923 3246 3461 Size: 399 415 378 232 32 76 323 215 Cuts at: 3461 4203 4288 5359 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 378 323 232 215 85 76 32 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2235 2463 4031 5761 2235 Size: 228 1568 1730 2616 Fragments arranged by size: 2616 1730 1568 228 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 2054 2526 3497 844 Size: 1206 4 472 971 3489 Fragments arranged by size: 3489 1206 971 472 4 BsrBI CCG'CTC Cuts at: 1394 1823 2041 4029 1394 Size: 429 218 1988 3507 Fragments arranged by size: 3507 1988 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 3202 1184 Size: 2018 4124 BstXI CCAn_nnnn'nTGG Cuts at: 2564 3191 2564 Size: 627 5515 BstYI r'GATC_y Cuts at: 1972 2595 3472 3839 4224 4241 5693 5779 5791 Size: 623 877 367 385 17 1452 86 12 Cuts at: 5791 6137 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 877 623 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2971 2971 Size: 6142 Bsu36I CC'TnA_GG Cuts at: 3348 3370 3348 Size: 22 6120 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 2728 2880 3213 3396 3414 Size: 552 939 264 460 152 333 183 18 Cuts at: 3414 513 Size: 3241 Fragments arranged by size: 3241 939 552 460 333 264 183 152 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2877 3210 3589 Size: 54 1425 50 275 18 1047 333 379 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 379 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2492 2687 4072 1353 Size: 731 408 195 1385 3423 Fragments arranged by size: 3423 1385 731 408 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3076 4729 5103 5249 387 Size: 1064 138 1487 1653 374 146 1280 Fragments arranged by size: 1653 1487 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2245 3008 3261 3342 4205 4802 5589 Size: 393 613 763 253 81 863 597 787 Cuts at: 5589 5857 1239 Size: 268 1524 Fragments arranged by size: 1524 863 787 763 613 597 393 268 253 81 EcoNI CCTnn'n_nnAGG Cuts at: 2522 2522 Size: 6142 EcoO109I rG'GnC_Cy Cuts at: 3352 3374 3352 Size: 22 6120 EcoRI G'AATT_C Cuts at: 2168 2638 3451 3829 2168 Size: 470 813 378 4481 Fragments arranged by size: 4481 813 470 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3134 3134 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2254 2268 2315 2571 5289 1678 Size: 576 14 47 256 2718 2531 Fragments arranged by size: 2718 2531 576 256 47 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2739 2771 3178 3210 4783 Size: 32 135 32 1167 32 407 32 1573 Cuts at: 4783 4815 4857 4889 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1573 1167 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 2188 748 Size: 1440 4702 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 2567 2567 Size: 6142 KpnI G_GTAC'C Cuts at: 2889 3431 3440 3467 2889 Size: 542 9 27 5564 Fragments arranged by size: 5564 542 27 9 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 5256 5440 1305 Size: 1329 2622 184 2007 Fragments arranged by size: 2622 2007 1329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3212 10 Size: 54 1425 343 1380 2940 Fragments arranged by size: 2940 1425 1380 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 3236 4100 4459 4618 518 Size: 648 1194 876 864 359 159 2042 Fragments arranged by size: 2042 1194 876 864 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2455 2891 3080 3558 4235 5383 5628 Size: 422 427 436 189 478 677 1148 245 Cuts at: 5628 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 478 436 427 422 245 189 NarI GG'CG_CC Cuts at: 2568 2568 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2004 2728 3213 3396 3414 513 Size: 552 939 724 485 183 18 3241 Fragments arranged by size: 3241 939 724 552 485 183 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3018 3500 5045 1132 Size: 97 1789 482 1545 2229 Fragments arranged by size: 2229 1789 1545 482 97 PasI CC'CwG_GG Cuts at: 1348 2959 1348 Size: 1611 4531 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5240 PfoI T'CCnGG_A Cuts at: 2193 2985 3006 2193 Size: 792 21 5329 Fragments arranged by size: 5329 792 21 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2206 2206 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3352 3374 3352 Size: 22 6120 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2156 2188 2156 Size: 32 6110 PstI C_TGCA'G Cuts at: 830 2061 2250 3494 830 Size: 1231 189 1244 3478 Fragments arranged by size: 3478 1244 1231 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2455 2455 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 2061 3494 2061 Size: 1433 4709 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2057 2246 3490 4625 5306 5497 826 Size: 219 1012 189 1244 1135 681 191 1471 Fragments arranged by size: 1471 1244 1135 1012 681 219 191 189 SfoI GGC'GCC Cuts at: 2569 2569 Size: 6142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 4246 5147 5409 820 Size: 197 926 206 2097 901 262 1553 Fragments arranged by size: 2097 1553 926 901 262 206 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2004 2728 2772 3213 3396 3414 3432 Size: 552 939 724 44 441 183 18 18 Cuts at: 3432 3581 513 Size: 149 3074 Fragments arranged by size: 3074 939 724 552 441 183 149 44 18 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3056 3215 4169 4186 4339 4524 4969 Size: 1255 1476 159 954 17 153 185 445 Cuts at: 4969 325 Size: 1498 Fragments arranged by size: 1498 1476 1255 954 445 185 159 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 4388 6100 Size: 274 80 33 51 1443 717 1694 1712 Cuts at: 6100 96 Size: 138 Fragments arranged by size: 1712 1694 1443 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3220 3220 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 2168 2501 4271 2168 Size: 333 1770 4039 Fragments arranged by size: 4039 1770 333 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI