Restriction map for
pFN21AE6476
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AlwNI ApaI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FauI FbaI FseI FspI HincII
HindII HpaI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Aor51HI 245 AGCGCT AGC^GCT
BalI 1205 TGGCCA TGG^CCA
BamHI 590 GGATCC G^GATCC
BbeI 562 GGCGCC GGCGC^C
BbrPI 199 CACGTG CAC^GTG
BbsI 208 GTCTTC GAAGAC(2/6)
BfaI 610 CTAG C^TAG
BglI 412 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 982 CTGGAG CTGGAG(16/14)
BpuAI 208 GTCTTC GAAGAC(2/6)
BsaAI 199 CACGTG [CT]AC^GT[GA]
BsaI 1189 GGTCTC GGTCTC(1/5)
BsrBI 34 CCGCTC CCGCTC(-3/-3)
Bst1107I 964 GTATAC GTA^TAC
BstEII 1197 GGTGACC G^GT[ACGT]ACC
BstPI 1197 GGTGACC G^GT[ACGT]ACC
BstYI 590 GGATCC [GA]^GATC[CT]
BstZ17I 964 GTATAC GTA^TAC
DpnII 591 GATC ^GATC
Eco47III 245 AGCGCT AGC^GCT
EcoNI 513 CCTGCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1197 GGTGACC G^GT[ACGT]ACC
HindIII 183 AAGCTT A^AGCTT
KasI 562 GGCGCC G^GCGCC
MaeI 610 CTAG C^TAG
MboI 591 GATC ^GATC
MflI 590 GGATCC [GA]^GATC[CT]
MluNI 1205 TGGCCA TGG^CCA
MscI 1205 TGGCCA TGG^CCA
NarI 562 GGCGCC GG^CGCC
NdeII 591 GATC ^GATC
NspI 1009 ACATGC [GA]CATG^[CT]
PleI 985 GAGTC GAGTC(4/5)
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PvuII 448 CAGCTG CAG^CTG
Sau3AI 591 GATC ^GATC
SbfI 51 CCTGCAGG CCTGCA^GG
SfoI 562 GGCGCC GGC^GCC
SmlI 144 CTTGAG C^T[CT][GA]AG
Sse8387I 51 CCTGCAGG CCTGCA^GG
TaqI 101 TCGA T^CGA
TfiI 69 GATTC G^A[AT]TC
TthHB8I 101 TCGA T^CGA
XcmI 1208 CCATGGCCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 590 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 163 GAATTC [GA]^AATT[CT]
633 GAATTC
AcyI 109 GACGCC G[GA]^CG[CT]C
562 GGCGCC
Alw44I 196 GTGCAC G^TGCAC
838 GTGCAC
AlwI 591 GATCC GGATC(4/5)
590 GGATC
AocI 1342 CCTGAGG CC^T[ACGT]AGG
1364 CCTCAGG
ApaLI 196 GTGCAC G^TGCAC
838 GTGCAC
ApoI 163 GAATTC [GA]^AATT[CT]
633 GAATTC
Asp700I 159 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
492 GAATGAGTTC
AspHI 196 GTGCAC G[AT]GC[AT]^C
838 GTGCAC
BanII 574 GGGCTC G[GA]GC[CT]^C
1237 GGGCTC
BcnI 587 CCCGG CC^[GC]GG
989 CCCGG
BsaHI 109 GACGCC G[GA]^CG[CT]C
562 GGCGCC
BsgI 811 CTGCAC GTGCAG(16/14)
994 CTGCAC
BsiHKAI 196 GTGCAC G[AT]GC[AT]^C
838 GTGCAC
BsmAI 627 GAGAC GTCTC(1/5)
1190 GTCTC
BsmI 849 GAATGC GAATGC(1/-1)
1034 GCATTC
BspHI 230 TCATGA T^CATGA
458 TCATGA
BstXI 552 CCACACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1179 CCAGATTTATGG
Bsu36I 1342 CCTGAGG CC^T[ACGT]AGG
1364 CCTCAGG
DraII 1346 AGGACCC [GA]G^G[ACGT]CC[CT]
1368 AGGACCC
EaeI 872 TGGCCG [CT]^GGCC[GA]
1205 TGGCCA
Eco81I 1342 CCTGAGG CC^T[ACGT]AGG
1364 CCTCAGG
EcoO109I 1346 AGGACCC [GA]G^G[ACGT]CC[CT]
1368 AGGACCC
EcoRI 163 GAATTC G^AATTC
633 GAATTC
HapII 588 CCGG C^CGG
990 CCGG
Hin1I 109 GACGCC G[GA]^CG[CT]C
562 GGCGCC
HinfI 69 GATTC G^A[ACGT]TC
985 GAGTC
HpaII 588 CCGG C^CGG
990 CCGG
MaeII 194 ACGT A^CGT
200 ACGT
MseI 509 TTAA T^TAA
1019 TTAA
MslI 351 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1227 CATCATTGTG
MspI 588 CCGG C^CGG
990 CCGG
NciI 587 CCCGG CC^[GC]GG
989 CCCGG
PpuMI 1346 AGGACCC [GA]G^G[AT]CC[CT]
1368 AGGACCC
PstI 52 CTGCAG CTGCA^G
241 CTGCAG
RcaI 230 TCATGA T^CATGA
458 TCATGA
SfcI 52 CTGCAG C^T[GA][CT]AG
241 CTGCAG
Tru9I 509 TTAA T^TAA
1019 TTAA
Tsp509I 164 AATT ^AATT
634 AATT
XmnI 159 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
492 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
Asp718I AvaII BanI BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinP1I
HphI ItaI KpnI Ksp632I MaeIII
MboII MnlI MspA1I MvaI MwoI
NcoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI