SEC61A2 ORF clone pFN21AE6390

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Halotag ORF Clone pFN21AE6390

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Clone info.

Species

human

Clone Name

pFN21AE6390 Sequence (with vector) Restriction Map

Product ID

FHC13542

ORF Sequence

DNA sequence (1254 bp)
Protein sequence (418 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6390

Symbol

SEC61A2

Description

Sec61 alpha 2 subunit (S. cerevisiae)

Original Clone ID

oc96390

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1254 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 418 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44358.1 6.2e-190 99.3 SEC61A2 CCDS7088.1 7.9e-190 100.0 SEC61A2 CCDS3046.1 1.1e-180 93.2 SEC61A1 CCDS44359.1 1.4e-166 94.7 SEC61A2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019561 40 74 PF10559 Translocon Sec61/SecY IPR002208 75 414 PF00344 SecY protein HMMTigr IPR002208 29 413 TIGR00967 SecY protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MGIKFLEVIKPFCAVLPEIQKPE 23 SECONDARY 23 2 33 LWTAITLFIFLVCCQIPLFGIMS 55 PRIMARY 23 3 72 NRGTLMELGISPIVTSGLIMQLL 94 SECONDARY 23 4 115 AQKLFGMIITIGQAIVYVMTGMY 137 PRIMARY 23 5 144 GAGICLLIIIQLFVAGLIVLLLD 166 PRIMARY 23 6 171 KGYGLGSGISLFIATNICETIVW 193 SECONDARY 23 7 239 LPNLMNLIATVFVFAVVIYFQGF 261 PRIMARY 23 8 294 QSALVSNLYVISQMLSVRFSGNF 316 SECONDARY 23 9 356 EDPVHVVVYIIFMLGSCAFFSK 377 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_037468 476 609213,617056 93.2 99.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6390 Download

>KIEE6390  1254 bp
ATGGGCATCAAATTTTTAGAAGTTATCAAACCATTCTGTGCAGTTCTACCAGAAATTCAG
AAACCGGAAAGGAAAATCCAGTTTAGAGAGAAGGTTCTGTGGACTGCTATAACGCTCTTC
ATTTTCTTAGTGTGTTGTCAGATCCCACTGTTTGGAATCATGTCATCAGATTCTGCAGAT
CCTTTCTACTGGATGAGAGTTATTCTGGCTTCCAATAGAGGAACTTTAATGGAATTGGGT
ATCTCCCCAATTGTAACATCTGGTTTGATTATGCAGTTGTTAGCTGGAGCCAAAATCATT
GAAGTTGGAGATACACCGAAAGATAGAGCTTTATTCAATGGAGCCCAGAAACTGTTTGGT
ATGATCATTACCATTGGGCAAGCCATTGTGTATGTCATGACGGGGATGTATGGGGACCCT
GCAGAAATGGGTGCTGGAATCTGTCTCCTGATCATCATTCAGTTGTTTGTTGCTGGTTTG
ATTGTGCTGCTGTTAGATGAGCTGCTACAGAAGGGTTACGGCTTGGGGTCTGGGATTTCC
CTCTTTATTGCCACCAACATCTGTGAGACCATTGTCTGGAAGGCCTTTAGTCCCACTACC
ATTAACACTGGCAGAGGTACTGAGTTTGAGGGTGCAGTCATAGCTCTGTTCCATTTGTTG
GCCACCAGGACGGACAAAGTCCGAGCTTTACGGGAGGCTTTTTATCGGCAGAACTTACCC
AATCTCATGAACCTCATTGCTACAGTTTTTGTGTTTGCTGTTGTTATATATTTCCAAGGA
TTTCGCGTTGATCTGCCCATTAAGTCGGCCCGTTACCGAGGACAGTACAGCAGCTACCCC
ATCAAACTCTTCTACACCTCCAACATCCCCATCATCCTCCAGTCGGCCCTGGTGTCCAAC
CTGTATGTTATTTCCCAGATGCTGTCTGTTCGATTTAGTGGCAACTTTTTAGTAAATTTA
CTAGGACAGTGGGCCGATGTCAGTGGGGGAGGACCCGCACGTTCTTACCCAGTTGGAGGC
CTTTGTTACTATCTTTCTCCTCCTGAGTCCATGGGCGCCATCTTTGAGGATCCTGTCCAT
GTCGTTGTTTATATCATCTTCATGTTGGGGTCATGTGCATTCTTCTCTAAGACATGGATT
GAAGTGTCTGGTTCCTCAGCCAAAGATGTAGCTAAACAGCTGAAAGAACAGCAGATGGTA
ATGAGGGGCCACCGAGATACCTCTATGGTTCATGAGCTTAATAGCGCTAGAGCG

ORF Protein

Cloned ORF protein sequence for pFN21AE6390 Download

>KIEE6390  418 aa
MGIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSAD
PFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFG
MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS
LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP
NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN
LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH
VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNSARA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6390 Download

>pFN21AE6390  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCATCAAATTTTTAGAAGTTATCAAACCATT
CTGTGCAGTTCTACCAGAAATTCAGAAACCGGAAAGGAAAATCCAGTTTAGAGAGAAGGT
TCTGTGGACTGCTATAACGCTCTTCATTTTCTTAGTGTGTTGTCAGATCCCACTGTTTGG
AATCATGTCATCAGATTCTGCAGATCCTTTCTACTGGATGAGAGTTATTCTGGCTTCCAA
TAGAGGAACTTTAATGGAATTGGGTATCTCCCCAATTGTAACATCTGGTTTGATTATGCA
GTTGTTAGCTGGAGCCAAAATCATTGAAGTTGGAGATACACCGAAAGATAGAGCTTTATT
CAATGGAGCCCAGAAACTGTTTGGTATGATCATTACCATTGGGCAAGCCATTGTGTATGT
CATGACGGGGATGTATGGGGACCCTGCAGAAATGGGTGCTGGAATCTGTCTCCTGATCAT
CATTCAGTTGTTTGTTGCTGGTTTGATTGTGCTGCTGTTAGATGAGCTGCTACAGAAGGG
TTACGGCTTGGGGTCTGGGATTTCCCTCTTTATTGCCACCAACATCTGTGAGACCATTGT
CTGGAAGGCCTTTAGTCCCACTACCATTAACACTGGCAGAGGTACTGAGTTTGAGGGTGC
AGTCATAGCTCTGTTCCATTTGTTGGCCACCAGGACGGACAAAGTCCGAGCTTTACGGGA
GGCTTTTTATCGGCAGAACTTACCCAATCTCATGAACCTCATTGCTACAGTTTTTGTGTT
TGCTGTTGTTATATATTTCCAAGGATTTCGCGTTGATCTGCCCATTAAGTCGGCCCGTTA
CCGAGGACAGTACAGCAGCTACCCCATCAAACTCTTCTACACCTCCAACATCCCCATCAT
CCTCCAGTCGGCCCTGGTGTCCAACCTGTATGTTATTTCCCAGATGCTGTCTGTTCGATT
TAGTGGCAACTTTTTAGTAAATTTACTAGGACAGTGGGCCGATGTCAGTGGGGGAGGACC
CGCACGTTCTTACCCAGTTGGAGGCCTTTGTTACTATCTTTCTCCTCCTGAGTCCATGGG
CGCCATCTTTGAGGATCCTGTCCATGTCGTTGTTTATATCATCTTCATGTTGGGGTCATG
TGCATTCTTCTCTAAGACATGGATTGAAGTGTCTGGTTCCTCAGCCAAAGATGTAGCTAA
ACAGCTGAAAGAACAGCAGATGGTAATGAGGGGCCACCGAGATACCTCTATGGTTCATGA
GCTTAATAGCGCTAGAGCGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT