(Circular) (Six-base) MAPSORT of: pFN21AE6390.seq Check: 9523 from: 1 to: 5959 pFN21AE6390 5959 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AfeI AGC'GCT Cuts at: 3250 3250 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2177 2356 2926 3702 5274 2177 Size: 179 570 776 1572 2862 Fragments arranged by size: 2862 1572 776 570 179 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2015 2058 2959 3268 3494 3646 5755 1493 Size: 522 43 901 309 226 152 2109 1697 Fragments arranged by size: 2109 1697 901 522 309 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3073 3289 3656 3073 Size: 216 367 5376 Fragments arranged by size: 5376 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3059 3280 4735 618 Size: 325 217 627 1272 221 1455 1842 Fragments arranged by size: 1842 1455 1272 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2350 3278 1391 Size: 959 928 4072 Fragments arranged by size: 4072 959 928 BbeI G_GCGC'C Cuts at: 3063 3063 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BbvCI CC'TCA_GC Cuts at: 3160 3160 Size: 5959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2367 2454 1548 Size: 819 87 5053 Fragments arranged by size: 5053 819 87 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3008 3690 4648 Size: 416 53 83 399 24 1591 682 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1591 958 682 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 2867 4619 1239 Size: 1071 557 1752 2579 Fragments arranged by size: 2579 1752 1071 557 Bpu10I CC'TnA_GC Cuts at: 3160 3160 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 4622 882 Size: 617 1065 2058 2219 Fragments arranged by size: 2219 2058 1065 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3060 3766 4148 4783 Size: 53 83 186 936 1527 706 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1527 1451 936 706 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2068 4392 4790 5064 5211 5622 2068 Size: 2324 398 274 147 411 2405 Fragments arranged by size: 2405 2324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3033 1186 Size: 1847 4112 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2064 2658 1163 Size: 573 328 594 4464 Fragments arranged by size: 4464 594 573 328 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4020 4105 5176 4020 Size: 85 1071 4803 Fragments arranged by size: 4803 1071 85 BsmI GAATG_Cn' Cuts at: 3122 3470 3563 3122 Size: 348 93 5518 Fragments arranged by size: 5518 348 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2350 3278 4020 4105 5176 1391 Size: 399 560 928 742 85 1071 2174 Fragments arranged by size: 2174 1071 928 742 560 399 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2400 2730 3235 3848 5578 2400 Size: 330 505 613 1730 2781 Fragments arranged by size: 2781 1730 613 505 330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3257 3846 1394 Size: 429 1434 589 3507 Fragments arranged by size: 3507 1434 589 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2739 4454 4628 65 Size: 1183 1491 1715 174 1396 Fragments arranged by size: 1715 1491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2145 2182 3073 3289 3656 4041 4058 5510 Size: 173 37 891 216 367 385 17 1452 Cuts at: 5510 5596 5608 5954 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 891 385 367 346 216 173 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3054 513 Size: 552 939 1050 3418 Fragments arranged by size: 3418 1050 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2664 3406 4295 Size: 54 1425 50 275 18 834 742 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 834 742 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2858 3889 1353 Size: 773 732 1031 3423 Fragments arranged by size: 3423 1031 773 732 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2867 4022 4619 5406 5674 1239 Size: 393 678 557 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 787 678 597 557 393 268 EcoO109I rG'GnC_Cy Cuts at: 2419 2996 2419 Size: 577 5382 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 3063 3252 5106 1678 Size: 1385 189 1854 2531 Fragments arranged by size: 2531 1854 1385 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 4600 4632 4674 Size: 32 135 32 711 32 2285 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 2285 711 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 3059 3059 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 2253 3558 2253 Size: 1305 4654 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2290 2889 2925 2998 5073 5257 1305 Size: 985 599 36 73 2075 184 2007 Fragments arranged by size: 2075 2007 985 599 184 73 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 10 Size: 54 1425 343 834 3303 Fragments arranged by size: 3303 1425 834 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3104 3917 4276 4435 518 Size: 648 1938 813 359 159 2042 Fragments arranged by size: 2042 1938 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3184 3375 4052 5200 5445 1606 Size: 1578 191 677 1148 245 2120 Fragments arranged by size: 2120 1578 1148 677 245 191 NarI GG'CG_CC Cuts at: 3060 3060 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2004 3054 513 Size: 552 939 1050 3418 Fragments arranged by size: 3418 1050 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2156 2580 2662 1108 Size: 1048 424 82 4405 Fragments arranged by size: 4405 1048 424 82 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2082 2114 4028 4060 5541 5573 2082 Size: 32 1914 32 1481 32 2468 Fragments arranged by size: 2468 1914 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2419 2996 2419 Size: 577 5382 PshAI GACnn'nnGTC Cuts at: 1613 2576 1613 Size: 963 4996 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2182 2428 3311 830 Size: 1352 246 883 3478 Fragments arranged by size: 3478 1352 883 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 3184 3184 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SanDI GG'GwC_CC Cuts at: 2419 2419 Size: 5959 SapI GCTCTTCn'nnn_ Cuts at: 2126 2126 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2178 2424 2510 2745 3307 4442 5123 Size: 219 1133 246 86 235 562 1135 681 Cuts at: 5123 5314 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1133 681 562 246 235 219 191 86 SfoI GGC'GCC Cuts at: 3061 3061 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2588 3024 1517 Size: 1071 436 4452 Fragments arranged by size: 4452 1071 436 StyI C'CwwG_G Cuts at: 513 1065 2004 2779 3054 3398 513 Size: 552 939 775 275 344 3074 Fragments arranged by size: 3074 939 775 552 344 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 3986 4003 4156 4341 4786 325 Size: 1255 843 1563 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 4205 5917 Size: 274 80 33 51 1443 852 1376 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1443 1376 852 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 2681 1316 Size: 1365 4594 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 2357 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI