################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BbrPI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI KspI MamI MluI MroI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 784 CGCG CG^CG AciI 995 CCGC CCGC(-3/-1) AcyI 1054 GGCGCC G[GA]^CG[CT]C Aor51HI 1243 AGCGCT AGC^GCT AspI 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 659 TGGCCA TGG^CCA BamHI 1068 GGATCC G^GATCC BanI 1054 GGCGCC G^G[CT][GA]CC BanII 341 GAGCCC G[GA]GC[CT]^C BbeI 1054 GGCGCC GGCGC^C BbvCI 1154 CCTCAGC CCTCAGC(-5/-2) BmyI 341 GAGCCC G[AGT]GC[ACT]^C BsaHI 1054 GGCGCC G[GA]^CG[CT]C BsaI 565 GAGACC GGTCTC(1/5) BsaWI 63 ACCGGA [AT]^CCGG[AT] BseRI 1037 CTCCTC GAGGAG(10/8) BsmI 1117 GCATTC GAATGC(1/-1) Bsp1286I 341 GAGCCC G[AGT]GC[ACT]^C BsrDI 735 CATTGC GCAATG(2/0) BstUI 784 CGCG CG^CG Cac8I 378 GCAAGC GC[ACGT]^[ACGT]GC DsaI 1049 CCATGG C^C[GA][CT]GG EaeI 659 TGGCCA [CT]^GGCC[GA] Eco47III 1243 AGCGCT AGC^GCT FauI 994 CCCGC CCCGC(4/6) HapII 64 CCGG C^CGG Hin1I 1054 GGCGCC G[GA]^CG[CT]C HpaII 64 CCGG C^CGG KasI 1054 GGCGCC G^GCGCC MaeII 999 ACGT A^CGT MfeI 248 CAATTG C^AATTG MluNI 659 TGGCCA TGG^CCA MscI 659 TGGCCA TGG^CCA MslI 1095 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1177 CAGCTG C[AC]G^C[GT]G MspI 64 CCGG C^CGG MunI 248 CAATTG C^AATTG MvnI 784 CGCG CG^CG MwoI 634 GCAGTCATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 1054 GGCGCC GG^CGCC NcoI 1049 CCATGG C^CATGG PflFI 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1045 GAGTC GAGTC(4/5) PshAI 567 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1177 CAGCTG CAG^CTG SapI 114 GCTCTTC GCTCTTC(1/4) SfoI 1054 GGCGCC GGC^GCC TaqI 930 TCGA T^CGA Tth111I 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 930 TCGA T^CGA XcmI 345 CCAGAAACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 617 GTAC GT^AC 825 GTAC AvaII 414 GGACC G^G[AT]CC 991 GGACC BclI 362 TGATCA T^GATCA 449 TGATCA BfaI 961 CTAG C^TAG 1247 CTAG BpmI 877 CTCCAG CTGGAG(16/14) 284 CTGGAG BsgI 38 GTGCAG GTGCAG(16/14) 632 GTGCAG BsmAI 565 GAGAC GTCTC(1/5) 443 GTCTC BsmFI 413 GGGAC GGGAC(10/14) 590 GTCCC BssKI 665 CCAGG ^CC[ACGT]GG 888 CCTGG BstNI 665 CCAGG CC^[AT]GG 888 CCTGG CfoI 1055 GCGC GCG^C 1244 GCGC DraII 413 GGGACCC [GA]G^G[ACGT]CC[CT] 990 AGGACCC EarI 115 CTCTTC CTCTTC(1/4) 847 CTCTTC EcoO109I 413 GGGACCC [GA]G^G[ACGT]CC[CT] 990 AGGACCC EcoRII 665 CCAGG ^CC[AT]GG 888 CCTGG EcoT14I 774 CCAAGG C^C[AT][AT]GG 1049 CCATGG FbaI 362 TGATCA T^GATCA 449 TGATCA HaeII 1054 GGCGCC [GA]GCGC^[CT] 1243 AGCGCT HhaI 1055 GCGC GCG^C 1244 GCGC HinP1I 1055 GCGC G^CGC 1244 GCGC Ksp632I 115 CTCTTC CTCTTC(1/4) 847 CTCTTC MaeI 961 CTAG C^TAG 1247 CTAG MvaI 665 CCAGG CC^[AT]GG 888 CCTGG PpuMI 413 GGGACCC [GA]G^G[AT]CC[CT] 990 AGGACCC PstI 173 CTGCAG CTGCA^G 419 CTGCAG RsaI 617 GTAC GT^AC 825 GTAC ScrFI 665 CCAGG CC^[ACGT]GG 888 CCTGG SfaNI 918 GATGC GCATC(5/9) 5 GCATC StuI 581 AGGCCT AGG^CCT 1017 AGGCCT StyI 774 CCAAGG C^C[AT][AT]GG 1049 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AcsI AluI AlwI AlwNI ApoI BbvI BsaJI BsiYI BslI BspHI BsrI BstF5I BstYI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII NlaIV PflMI RcaI Sau3AI Sau96I SfcI TfiI Tru9I TseI Tsp509I TspRI Van91I XhoII