SEC22A ORF clone pFN21AE6378

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Halotag ORF Clone pFN21AE6378

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Clone info.

Species

human

Clone Name

pFN21AE6378 Sequence (with vector) Restriction Map

Product ID

FHC21671

ORF Sequence

DNA sequence (921 bp)
Protein sequence (307 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6378

Symbol

SEC22A

Alias : SEC22L2

Description

SEC22 homolog A, vesicle trafficking protein

Original Clone ID

oc96378

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 921 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 307 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3021.1 1.4e-138 100.0 SEC22A CCDS2700.1 3.2e-47 40.8 SEC22C CCDS2699.1 5.3e-33 39.1 SEC22C CCDS56246.1 6.1e-30 39.1 SEC22C CCDS83523.1 1.1e-18 40.8 SEC22B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition ProfileScan IPR010908 8 119 PS50859 Longin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 186 EPATLSGIVGFILSLLCGALNLI 208 PRIMARY 23 2 226 NYIIAFFLGTAACLYQCYLLVYY 248 SECONDARY 23 3 251 WRNVKSFLTFGLICLCNMYLYEL 273 SECONDARY 23 4 277 WQLFFHVTVGAFVTLQIWLRQA 298 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011510978 307 612442 100.0 100.0 XP_011510975 307 612442 100.0 100.0 XP_011510977 307 612442 100.0 100.0 NP_036562 307 612442 100.0 100.0 NP_116752 303 604028 40.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6378 Download

>KIEE6378  921 bp
ATGTCTATGATTTTATCTGCCTCAGTCATTCGTGTCAGAGATGGACTGCCACTTTCTGCT
TCTACTGATTATGAACAAAGCACAGGAATGCAGGAGTGCAGAAAGTATTTTAAAATGCTT
TCGAGGAAACTTGCTCAACTTCCTGATAGATGTACACTGAAAACTGGACATTATAACATT
AATTTTATTAGCTCTCTGGGAGTGAGCTACATGATGTTGTGCACTGAAAATTACCCAAAT
GTTCTCGCCTTCTCTTTCCTGGATGAGCTTCAGAAGGAGTTCATTACTACTTATAACATG
ATGAAGACAAATACTGCTGTCAGACCATACTGTTTCATTGAATTTGATAACTTCATTCAG
AGGACCAAGCAGCGATATAATAATCCCAGGTCTCTTTCAACAAAGATAAATCTTTCTGAC
ATGCAGACGGAAATCAAGCTGAGGCCTCCTTATCAAATTTCCATGTGCGAACTGGGGTCA
GCCAATGGAGTCACATCAGCATTTTCTGTTGACTGTAAAGGTGCTGGTAAGATTTCTTCT
GCTCACCAGCGACTGGAACCAGCAACTCTGTCAGGGATTGTAGGATTTATCCTTAGTCTT
TTATGTGGAGCTCTGAATTTAATTCGAGGCTTTCATGCTATAGAAAGTCTCCTGCAGAGT
GATGGTGATGATTTTAATTACATCATTGCATTTTTCCTTGGAACAGCAGCCTGCCTTTAC
CAGTGTTATTTACTTGTCTACTACACCGGCTGGCGGAATGTCAAATCTTTTTTGACTTTT
GGCTTAATCTGTCTATGCAACATGTATCTCTATGAACTGCGCAACCTCTGGCAGCTTTTC
TTTCATGTGACTGTGGGAGCATTTGTTACACTACAGATCTGGCTAAGGCAAGCCCAGGGC
AAGGCTCCCGATTATGATGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE6378 Download

>KIEE6378  307 aa
MSMILSASVIRVRDGLPLSASTDYEQSTGMQECRKYFKMLSRKLAQLPDRCTLKTGHYNI
NFISSLGVSYMMLCTENYPNVLAFSFLDELQKEFITTYNMMKTNTAVRPYCFIEFDNFIQ
RTKQRYNNPRSLSTKINLSDMQTEIKLRPPYQISMCELGSANGVTSAFSVDCKGAGKISS
AHQRLEPATLSGIVGFILSLLCGALNLIRGFHAIESLLQSDGDDFNYIIAFFLGTAACLY
QCYLLVYYTGWRNVKSFLTFGLICLCNMYLYELRNLWQLFFHVTVGAFVTLQIWLRQAQG
KAPDYDV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6378 Download

>pFN21AE6378  5626 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTATGATTTTATCTGCCTCAGTCATTCGTGT
CAGAGATGGACTGCCACTTTCTGCTTCTACTGATTATGAACAAAGCACAGGAATGCAGGA
GTGCAGAAAGTATTTTAAAATGCTTTCGAGGAAACTTGCTCAACTTCCTGATAGATGTAC
ACTGAAAACTGGACATTATAACATTAATTTTATTAGCTCTCTGGGAGTGAGCTACATGAT
GTTGTGCACTGAAAATTACCCAAATGTTCTCGCCTTCTCTTTCCTGGATGAGCTTCAGAA
GGAGTTCATTACTACTTATAACATGATGAAGACAAATACTGCTGTCAGACCATACTGTTT
CATTGAATTTGATAACTTCATTCAGAGGACCAAGCAGCGATATAATAATCCCAGGTCTCT
TTCAACAAAGATAAATCTTTCTGACATGCAGACGGAAATCAAGCTGAGGCCTCCTTATCA
AATTTCCATGTGCGAACTGGGGTCAGCCAATGGAGTCACATCAGCATTTTCTGTTGACTG
TAAAGGTGCTGGTAAGATTTCTTCTGCTCACCAGCGACTGGAACCAGCAACTCTGTCAGG
GATTGTAGGATTTATCCTTAGTCTTTTATGTGGAGCTCTGAATTTAATTCGAGGCTTTCA
TGCTATAGAAAGTCTCCTGCAGAGTGATGGTGATGATTTTAATTACATCATTGCATTTTT
CCTTGGAACAGCAGCCTGCCTTTACCAGTGTTATTTACTTGTCTACTACACCGGCTGGCG
GAATGTCAAATCTTTTTTGACTTTTGGCTTAATCTGTCTATGCAACATGTATCTCTATGA
ACTGCGCAACCTCTGGCAGCTTTTCTTTCATGTGACTGTGGGAGCATTTGTTACACTACA
GATCTGGCTAAGGCAAGCCCAGGGCAAGGCTCCCGATTATGATGTCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT