Restriction map for
pFN21AE6378
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 736 GTCTAC GT^[AC][GT]AC
AciI 753 GCGG CCGC(-3/-1)
AfaI 152 GTAC GT^AC
AflIII 800 ACATGT A^C[GA][CT]GT
Alw44I 219 GTGCAC G^TGCAC
AlwNI 547 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 219 GTGCAC G^TGCAC
AseI 178 ATTAAT AT^TAAT
AsnI 178 ATTAAT AT^TAAT
Asp700I 273 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 362 GGACC G^G[AT]CC
AviII 818 TGCGCA TGC^GCA
BanII 608 GAGCTC G[GA]GC[CT]^C
BbsI 303 GAAGAC GAAGAC(2/6)
BbvCI 438 GCTGAGG CCTCAGC(-5/-2)
BglII 875 AGATCT A^GATCT
BpuAI 303 GAAGAC GAAGAC(2/6)
BsaI 389 GGTCTC GGTCTC(1/5)
BsgI 96 GTGCAG GTGCAG(16/14)
BsmI 86 GAATGC GAATGC(1/-1)
Bsp1407I 151 TGTACA T^GTACA
BspLU11I 800 ACATGT A^CATGT
BsrDI 684 CATTGC GCAATG(2/0)
BsrFI 745 ACCGGC [GA]^CCGG[CT]
BsrGI 151 TGTACA T^GTACA
BstF5I 261 GGATG GGATG(2/0)
BstYI 875 AGATCT [GA]^GATC[CT]
CfoI 819 GCGC GCG^C
Cfr10I 745 ACCGGC [GA]^CCGG[CT]
Cfr13I 362 GGACC G^G[ACGT]CC
DpnII 876 GATC ^GATC
DraI 109 TTTAAA TTT^AAA
EcoT14I 696 CCTTGG C^C[AT][AT]GG
FokI 261 GGATG GGATG(9/13)
FspI 818 TGCGCA TGC^GCA
HaeIII 443 GGCC GG^CC
HapII 746 CCGG C^CGG
HhaI 819 GCGC GCG^C
HinP1I 819 GCGC G^CGC
HincII 508 GTTGAC GT[CT]^[GA]AC
HindII 508 GTTGAC GT[CT]^[GA]AC
HinfI 488 GAGTC G^A[ACGT]TC
HpaII 746 CCGG C^CGG
MboI 876 GATC ^GATC
MflI 875 AGATCT [GA]^GATC[CT]
MslI 81 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 746 CCGG C^CGG
NdeII 876 GATC ^GATC
PflMI 546 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 488 GAGTC GAGTC(4/5)
PshBI 178 ATTAAT AT^TAAT
PstI 652 CTGCAG CTGCA^G
RsaI 152 GTAC GT^AC
SacI 608 GAGCTC GAGCT^C
Sau3AI 876 GATC ^GATC
Sau96I 362 GGACC G^G[ACGT]CC
SspBI 151 TGTACA T^GTACA
StuI 442 AGGCCT AGG^CCT
StyI 696 CCTTGG C^C[AT][AT]GG
Van91I 546 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 461 CCATGTGCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 875 AGATCT [GA]^GATC[CT]
XmnI 273 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AspHI 219 GTGCAC G[AT]GC[AT]^C
608 GAGCTC
BmyI 219 GTGCAC G[AGT]GC[ACT]^C
608 GAGCTC
BsiHKAI 219 GTGCAC G[AT]GC[AT]^C
608 GAGCTC
BsmAI 390 GTCTC GTCTC(1/5)
647 GTCTC
Bsp1286I 219 GTGCAC G[AGT]GC[ACT]^C
608 GAGCTC
HphI 664 GGTGA GGTGA(8/7)
543 TCACC
MboII 303 GAAGA GAAGA(8/7)
535 TCTTC
NlaIV 555 GGAACC GG[ACGT]^[ACGT]CC
903 GGCTCC
NspI 419 ACATGC [GA]CATG^[CT]
800 ACATGT
TaqI 121 TCGA T^CGA
624 TCGA
Tsp45I 490 GTCAC ^GT[GC]AC
847 GTGAC
TthHB8I 121 TCGA T^CGA
624 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I DdeI EcoRII Fnu4HI ItaI
MaeIII MnlI MseI MvaI NlaIII
ScrFI SfcI Tru9I TseI Tsp509I
TspRI