(Circular) (Six-base) MAPSORT of: pFN21AE6378.seq Check: 1408 from: 1 to: 5626 pFN21AE6378 5626 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 2969 1937 Size: 805 227 4594 Fragments arranged by size: 4594 805 227 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 3689 5073 5341 1632 Size: 626 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 626 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 2805 4525 5502 1203 Size: 1602 1720 977 1327 Fragments arranged by size: 1720 1602 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2108 1184 Size: 924 4702 AlwNI CAG_nnn'CTG Cuts at: 2557 3369 4941 2557 Size: 812 1572 3242 Fragments arranged by size: 3242 1572 812 ApaLI G'TGCA_C Cuts at: 2224 3683 4839 2224 Size: 1459 1156 3011 Fragments arranged by size: 3011 1459 1156 ApoI r'AATT_y Cuts at: 1493 2345 2460 2620 2935 3161 3313 5422 1493 Size: 852 115 160 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 315 226 160 152 115 AseI AT'TA_AT Cuts at: 160 1032 2184 4180 160 Size: 872 1152 1996 1606 Fragments arranged by size: 1996 1606 1152 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2951 1870 Size: 73 1008 4545 Fragments arranged by size: 4545 1008 73 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2947 4402 618 Size: 325 217 627 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 325 217 BanII G_rGCy'C Cuts at: 1391 2617 2945 1391 Size: 1226 328 4072 Fragments arranged by size: 4072 1226 328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 928 Size: 971 416 4239 Fragments arranged by size: 4239 971 416 BbvCI CC'TCA_GC Cuts at: 2444 2444 Size: 5626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4237 136 Size: 107 122 71 631 605 2565 1525 Fragments arranged by size: 2565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2880 5621 2880 Size: 2741 2885 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2228 3687 4843 1790 Size: 438 1459 1156 2573 Fragments arranged by size: 2573 1459 1156 438 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2486 3357 4315 Size: 416 53 83 399 24 1069 871 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1069 958 871 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4286 1239 Size: 3047 2579 Bpu10I CC'TnA_GC Cuts at: 2444 2888 2444 Size: 444 5182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2400 4289 882 Size: 617 901 1889 2219 Fragments arranged by size: 2219 1889 901 617 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3433 3815 4450 275 Size: 53 83 186 936 1900 382 635 1451 Fragments arranged by size: 1900 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2853 2883 1178 Size: 30 165 30 1450 30 3921 Fragments arranged by size: 3921 1450 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5626 BseYI C'CCAG_C Cuts at: 4829 5325 4829 Size: 496 5130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 1163 Size: 573 386 4667 Fragments arranged by size: 4667 573 386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3076 3837 4865 1540 Size: 275 186 1075 761 1028 2301 Fragments arranged by size: 2301 1075 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2228 2617 3687 3772 4843 2228 Size: 389 1070 85 1071 3011 Fragments arranged by size: 3011 1071 1070 389 85 BsmI GAATG_Cn' Cuts at: 2097 3137 3230 2097 Size: 1040 93 4493 Fragments arranged by size: 4493 1040 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2617 2945 3687 3772 4843 1391 Size: 399 438 389 328 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 399 389 328 85 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2688 4121 4295 65 Size: 1183 1440 1433 174 1396 Fragments arranged by size: 1440 1433 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2750 4270 4457 1700 Size: 110 940 1520 187 2869 Fragments arranged by size: 2869 1520 940 187 110 BsrGI T'GTAC_A Cuts at: 96 2156 96 Size: 2060 3566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2880 2956 3323 3708 3725 5177 5263 5275 Size: 908 76 367 385 17 1452 86 12 Cuts at: 5275 5621 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 908 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5074 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3936 3956 3142 Size: 794 20 4812 Fragments arranged by size: 4812 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2116 2930 2116 Size: 814 4812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3073 3962 5591 Size: 54 1425 50 275 18 1243 889 1629 Cuts at: 5591 8 Size: 43 Fragments arranged by size: 1629 1425 1243 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3556 1353 Size: 2203 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2773 4213 4587 4733 387 Size: 1064 138 1184 1440 374 146 1280 Fragments arranged by size: 1440 1280 1184 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2615 2615 Size: 5626 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 3689 4286 5073 5341 1239 Size: 393 626 1431 597 787 268 1524 Fragments arranged by size: 1524 1431 787 626 597 393 268 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FspI TGC'GCA Cuts at: 2825 4132 2825 Size: 1307 4319 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373 Size: 32 135 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2515 2970 3216 669 Size: 988 281 577 455 246 3079 Fragments arranged by size: 3079 988 577 455 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4740 4924 1305 Size: 3435 184 2007 Fragments arranged by size: 3435 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2090 3584 3943 4102 518 Size: 648 843 81 1494 359 159 2042 Fragments arranged by size: 2042 1494 843 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 3042 3719 4867 5112 1606 Size: 1436 677 1148 245 2120 Fragments arranged by size: 2120 1436 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5074 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2428 2809 2984 4529 1132 Size: 97 1199 381 175 1545 2229 Fragments arranged by size: 2229 1545 1199 381 175 97 PasI CC'CwG_GG Cuts at: 1348 2899 1348 Size: 1551 4075 PciI A'CATG_T Cuts at: 2805 4525 2805 Size: 1720 3906 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 1108 Size: 1449 4177 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 5626 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 2178 2298 3196 2178 Size: 120 898 4608 Fragments arranged by size: 4608 898 120 PstI C_TGCA'G Cuts at: 830 2661 2978 830 Size: 1831 317 3478 Fragments arranged by size: 3478 1831 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SacI G_AGCT'C Cuts at: 2617 2617 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2643 2657 2876 2974 4109 4790 4981 Size: 219 1598 14 219 98 1135 681 191 Cuts at: 4981 826 Size: 1471 Fragments arranged by size: 1598 1471 1135 681 219 219 191 98 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 4631 4893 820 Size: 197 926 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 2449 1517 Size: 932 4694 StyI C'CwwG_G Cuts at: 513 1065 2701 3065 513 Size: 552 1636 364 3074 Fragments arranged by size: 3074 1636 552 364 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3653 3670 3823 4008 4453 325 Size: 1255 2073 17 153 185 445 1498 Fragments arranged by size: 2073 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2156 3872 5584 Size: 274 80 33 51 1443 179 1716 1712 Cuts at: 5584 96 Size: 138 Fragments arranged by size: 1716 1712 1443 274 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 2282 3755 2282 Size: 1473 4153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI