AQP12A ORF clone pFN21AE6254

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Halotag ORF Clone pFN21AE6254

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Clone info.

Species

human

Clone Name

pFN21AE6254 Sequence (with vector) Restriction Map

Product ID

FHC21400

ORF Sequence

DNA sequence (885 bp)
Protein sequence (295 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6254

Symbol

AQP12A

Alias : AQP-12, AQP12, AQPX2

Description

aquaporin 12A

Original Clone ID

oc96254

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 885 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 295 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46560.1 1e-104 95.1 AQP12B CCDS8251.1 1.5e-16 32.6 AQP11 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023265 1 10 PR02025 Aquaporin 12 IPR023265 18 28 PR02025 Aquaporin 12 IPR023265 32 41 PR02025 Aquaporin 12 IPR023265 42 55 PR02025 Aquaporin 12 IPR000425 61 85 PR00783 Major intrinsic protein IPR023265 87 99 PR02025 Aquaporin 12 IPR000425 98 117 PR00783 Major intrinsic protein IPR023265 114 127 PR02025 Aquaporin 12 IPR023265 131 140 PR02025 Aquaporin 12 IPR023265 141 150 PR02025 Aquaporin 12 IPR023265 168 178 PR02025 Aquaporin 12 IPR000425 181 203 PR00783 Major intrinsic protein IPR023265 207 219 PR02025 Aquaporin 12 IPR000425 221 241 PR00783 Major intrinsic protein IPR023265 238 250 PR02025 Aquaporin 12 IPR023265 251 264 PR02025 Aquaporin 12 HMMPfam IPR000425 64 238 PF00230 Major intrinsic protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 8 LSFFFATFALCEAARRASKALLP 30 SECONDARY 23 2 54 DFGPDLLLTLLFLLFLAHGVTLD 76 PRIMARY 23 3 104 AAQGLGMQAACTLMRLCWAWELS 126 SECONDARY 23 4 149 HGALVEAACAFCFHLTLLHLRHS 171 SECONDARY 23 5 184 VTVTAYTAGPFTSAFFNPALAAS 206 SECONDARY 23 6 221 QVYWLGPLTGMVLAVLLHQGRLP 243 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_945349 295 609789 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6254 Download

>KIEE6254  885 bp
ATGGCAGGTCTTAACGTGTCCCTCTCCTTCTTCTTTGCCACCTTCGCCCTCTGTGAGGCG
GCCAGGCGGGCCTCCAAGGCCCTGCTCCCAGTGGGCGCCTATGAAGTCTTCGCCCGGGAG
GCGATGAGGACGCTGGTCGAGCTCGGGCCCTGGGCTGGGGACTTTGGGCCTGACCTGCTG
CTCACCCTGCTCTTCCTGCTCTTCCTGGCGCACGGGGTCACCTTGGACGGGGCCTCGGCC
AACCCCACTGTGTCCCTGCAGGAGTTCCTCATGGCCGAGCAGTCTCTGCCTGGCACGCTG
TTGAAGCTGGCGGCACAGGGGCTGGGCATGCAGGCCGCCTGCACCCTGATGCGCCTCTGC
TGGGCCTGGGAGCTCAGTGACCTGCACCTGCTGCAGAGCCTCATGGCCCAGAGCTGCAGC
TCGGCCCTGCGCACATCCGTGCCCCACGGGGCGCTTGTGGAGGCCGCCTGCGCCTTTTGT
TTCCATCTGACCCTCCTGCACCTGCGGCACAGTCCTCCCGCCTACAGCGGGCCCGCTGTG
GCTCTGTTGGTCACCGTCACGGCCTACACGGCCGGGCCCTTCACGTCTGCCTTCTTCAAC
CCTGCCCTGGCCGCCTCTGTGACCTTTGCCTGCTCGGGACACACCTTACTGGAGTACGTG
CAGGTGTACTGGCTGGGCCCTCTGACAGGGATGGTCCTGGCTGTGCTGCTGCACCAGGGC
CGCCTTCCCCACCTTTTCCAGAGGAACCTGTTCTACGGCCAGAAGAACAAGTACCGAGCA
CCCCGAGGGAAGCCGGCCCCGGCCTCAGGGGACACCCAGACCCCTGCAAAGGGGTCCAGT
GTCCGGGAGCCTGGGCGCAGTGGTGTTGAGGGGCCACATTCCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE6254 Download

>KIEE6254  295 aa
MAGLNVSLSFFFATFALCEAARRASKALLPVGAYEVFAREAMRTLVELGPWAGDFGPDLL
LTLLFLLFLAHGVTLDGASANPTVSLQEFLMAEQSLPGTLLKLAAQGLGMQAACTLMRLC
WAWELSDLHLLQSLMAQSCSSALRTSVPHGALVEAACAFCFHLTLLHLRHSPPAYSGPAV
ALLVTVTAYTAGPFTSAFFNPALAASVTFACSGHTLLEYVQVYWLGPLTGMVLAVLLHQG
RLPHLFQRNLFYGQKNKYRAPRGKPAPASGDTQTPAKGSSVREPGRSGVEGPHSS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6254 Download

>pFN21AE6254  5590 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGTCTTAACGTGTCCCTCTCCTTCTTCTT
TGCCACCTTCGCCCTCTGTGAGGCGGCCAGGCGGGCCTCCAAGGCCCTGCTCCCAGTGGG
CGCCTATGAAGTCTTCGCCCGGGAGGCGATGAGGACGCTGGTCGAGCTCGGGCCCTGGGC
TGGGGACTTTGGGCCTGACCTGCTGCTCACCCTGCTCTTCCTGCTCTTCCTGGCGCACGG
GGTCACCTTGGACGGGGCCTCGGCCAACCCCACTGTGTCCCTGCAGGAGTTCCTCATGGC
CGAGCAGTCTCTGCCTGGCACGCTGTTGAAGCTGGCGGCACAGGGGCTGGGCATGCAGGC
CGCCTGCACCCTGATGCGCCTCTGCTGGGCCTGGGAGCTCAGTGACCTGCACCTGCTGCA
GAGCCTCATGGCCCAGAGCTGCAGCTCGGCCCTGCGCACATCCGTGCCCCACGGGGCGCT
TGTGGAGGCCGCCTGCGCCTTTTGTTTCCATCTGACCCTCCTGCACCTGCGGCACAGTCC
TCCCGCCTACAGCGGGCCCGCTGTGGCTCTGTTGGTCACCGTCACGGCCTACACGGCCGG
GCCCTTCACGTCTGCCTTCTTCAACCCTGCCCTGGCCGCCTCTGTGACCTTTGCCTGCTC
GGGACACACCTTACTGGAGTACGTGCAGGTGTACTGGCTGGGCCCTCTGACAGGGATGGT
CCTGGCTGTGCTGCTGCACCAGGGCCGCCTTCCCCACCTTTTCCAGAGGAACCTGTTCTA
CGGCCAGAAGAACAAGTACCGAGCACCCCGAGGGAAGCCGGCCCCGGCCTCAGGGGACAC
CCAGACCCCTGCAAAGGGGTCCAGTGTCCGGGAGCCTGGGCGCAGTGGTGTTGAGGGGCC
ACATTCCAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT