Restriction map for
pFN21AE6254
(Circular) (Six-base) MAPSORT of: pFN21AE6254.seq Check: 1542 from: 1 to: 5590
pFN21AE6254 5590 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2400 2514 2656 2400
Size: 114 142 5334
Fragments arranged by size:
5334 142 114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 2019 4489 5466 1203
Size: 816 2470 977 1327
Fragments arranged by size:
2470 1327 977 816
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2441 1166
Size: 1275 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2369 2459 1184
Size: 1185 90 4315
Fragments arranged by size:
4315 1185 90
AlwNI CAG_nnn'CTG
Cuts at: 2290 2326 3333 4905 2290
Size: 36 1007 1572 2975
Fragments arranged by size:
2975 1572 1007 36
ApaI G_GGCC'C
Cuts at: 2154 2538 2583 2684 2154
Size: 384 45 101 5060
Fragments arranged by size:
5060 384 101 45
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2147 2638 2787 2915 1870
Size: 73 175 29 491 149 128 4545
Fragments arranged by size:
4545 491 175 149 128 73 29
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2911 4366 618
Size: 325 217 627 312 812 1455 1842
Fragments arranged by size:
1842 1455 812 627 325 312 217
BanII G_rGCy'C
Cuts at: 1391 2148 2154 2379 2538 2583 2684 2909 1391
Size: 757 6 225 159 45 101 225 4072
Fragments arranged by size:
4072 757 225 225 159 101 45 6
BbeI G_GCGC'C
Cuts at: 2103 2103
Size: 5590
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 928
Size: 971 205 4414
Fragments arranged by size:
4414 971 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2081 2123
Size: 107 122 71 631 605 400 9 42
Cuts at: 2123 2573 4201 136
Size: 450 1628 1525
Fragments arranged by size:
1628 1525 631 605 450 400 122 107
71 42 9
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2448 2538 2583 2684 3651 4807 1790
Size: 364 294 90 45 101 967 1156 2573
Fragments arranged by size:
2573 1156 967 364 294 101 90 45
BmgBI CAC'GTC
Cuts at: 1318 2589 1318
Size: 1271 4319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 3321 4279
Size: 416 53 83 399 24 670 1234 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1234 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 4250 1239
Size: 1436 1575 2579
Fragments arranged by size:
2579 1575 1436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 3381 493
Size: 711 1458 719 2702
Fragments arranged by size:
2702 1458 719 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 3397 3779 4414
Size: 53 83 186 936 567 1297 382 635
Cuts at: 4414 275
Size: 1451
Fragments arranged by size:
1451 1297 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5590
BseYI C'CCAG_C
Cuts at: 2159 2326 2364 2677 4793 5289 2159
Size: 167 38 313 2116 496 2460
Fragments arranged by size:
2460 2116 496 313 167 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 2372 2486 2684 2699 1163
Size: 573 593 43 114 198 15 4054
Fragments arranged by size:
4054 593 573 198 114 43 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2577 3040 3801 4829 1540
Size: 275 186 576 463 761 1028 2301
Fragments arranged by size:
2301 1028 761 576 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2379 2785 3651 3736 4807 2148
Size: 231 406 866 85 1071 2931
Fragments arranged by size:
2931 1071 866 406 231 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2154 2379 2448 2538 2583 2684
Size: 399 358 6 225 69 90 45 101
Cuts at: 2684 2785 2909 3651 3736 4807 1391
Size: 101 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 225 124 101
101 90 85 69 45 6
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2187 2394 2400 2514 2656 2945 844
Size: 1156 187 207 6 114 142 289 3489
Fragments arranged by size:
3489 1156 289 207 187 142 114 6
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2797 4234 4421 1700
Size: 110 987 1437 187 2869
Fragments arranged by size:
2869 1437 987 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2290 2395 794
Size: 1496 105 3989
Fragments arranged by size:
3989 1496 105
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 2221 2554 1184
Size: 1037 333 4220
Fragments arranged by size:
4220 1037 333
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2809 2809
Size: 5590
BtgI C'CryG_G
Cuts at: 513 1065 2004 2449 513
Size: 552 939 445 3654
Fragments arranged by size:
3654 939 552 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2141 487
Size: 683 56 915 3936
Fragments arranged by size:
3936 915 683 56
BtsI GCAGTG_nn'
Cuts at: 2869 3106 3900 3920 2869
Size: 237 794 20 4539
Fragments arranged by size:
4539 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2241 2277
Size: 54 1425 50 275 18 234 177 36
Cuts at: 2277 2574 2613 2761 3037 3926 5555 8
Size: 297 39 148 276 889 1629 43
Fragments arranged by size:
1629 1425 889 297 276 275 234 177
148 54 50 43 39 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2574 3037 1537
Size: 275 762 463 4090
Fragments arranged by size:
4090 762 463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2210 3520 1353
Size: 848 9 1310 3423
Fragments arranged by size:
3423 1310 848 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2377 2146
Size: 231 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2675 3653 4250 5037 5305 1239
Size: 393 1043 978 597 787 268 1524
Fragments arranged by size:
1524 1043 978 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2688 2057
Size: 631 4959
EcoO109I rG'GnC_Cy
Cuts at: 2083 2151 2235 2580 2681 2083
Size: 68 84 345 101 4992
Fragments arranged by size:
4992 345 101 84 68
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 2435 4096 2435
Size: 1661 3929
HaeII r_GCGC'y
Cuts at: 1678 2103 2459 4737 1678
Size: 425 356 2278 2531
Fragments arranged by size:
2531 2278 425 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 4231 4263 4305
Size: 32 135 32 650 32 1977 32 42
Cuts at: 4305 4337 1373
Size: 32 2626
Fragments arranged by size:
2626 1977 650 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KasI G'GCGC_C
Cuts at: 2099 2099
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3768
Fragments arranged by size:
3768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2441 3548 3907 4066 518
Size: 648 1185 90 1107 359 159 2042
Fragments arranged by size:
2042 1185 1107 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2532 2540 3006 3683 4831 5076 1606
Size: 926 8 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 677 466 245 8
NaeI GCC'GGC
Cuts at: 2799 2799
Size: 5590
NarI GG'CG_CC
Cuts at: 2100 2100
Size: 5590
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NgoMIV G'CCGG_C
Cuts at: 2797 2797
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2335 2948 4493 1132
Size: 97 1106 613 1545 2229
Fragments arranged by size:
2229 1545 1106 613 97
PasI CC'CwG_GG
Cuts at: 1348 2154 1348
Size: 806 4784
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PfoI T'CCnGG_A
Cuts at: 2847 2847
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2138 1613
Size: 525 5065
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2150 2534 2579 2680 2150
Size: 384 45 101 5060
Fragments arranged by size:
5060 384 101 45
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 5558
PstI C_TGCA'G
Cuts at: 830 2265 2400 2423 2942 830
Size: 1435 135 23 519 3478
Fragments arranged by size:
3478 1435 519 135 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
SacI G_AGCT'C
Cuts at: 2148 2379 2148
Size: 231 5359
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2210 2201
Size: 9 5581
SbfI CC_TGCA'GG
Cuts at: 2265 2942 2265
Size: 677 4913
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2261 2396 2419 2527 2938 4073 4754
Size: 219 1216 135 23 108 411 1135 681
Cuts at: 4754 4945 826
Size: 191 1471
Fragments arranged by size:
1471 1216 1135 681 411 219 191 135
108 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 2072 2081 2573 2072
Size: 9 492 5089
Fragments arranged by size:
5089 492 9
SfoI GGC'GCC
Cuts at: 2101 2101
Size: 5590
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2120 2917 1872
Size: 248 797 4545
Fragments arranged by size:
4545 797 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3694 4595 4857 820
Size: 197 926 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2335 2948 1132
Size: 1203 613 3774
Fragments arranged by size:
3774 1203 613
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2226 3029 513
Size: 552 939 75 147 803 3074
Fragments arranged by size:
3074 939 803 552 147 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2834 3617 3634 3787 3972 4417 325
Size: 1255 1254 783 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1254 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 3836 5548
Size: 274 80 33 51 1443 693 1166 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1712 1443 1166 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2118 2915 1870
Size: 248 797 4545
Fragments arranged by size:
4545 797 248
XmnI GAAnn'nnTTC
Cuts at: 2753 3719 2753
Size: 966 4624
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PvuII RsrII SacII SanDI SexAI SrfI SwaI XcmI