(Linear) (Six-base) MAPSORT of: KIEE6254.seq Check: 7135 from: 1 to: 885 KIEE6254.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 395 509 651 885 Size: 395 114 142 234 Fragments arranged by size: 395 234 142 114 AflIII A'CryG_T Cuts at: 0 14 885 Size: 14 871 AleI CACnn'nnGTG Cuts at: 0 436 885 Size: 436 449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 364 454 885 Size: 364 90 431 Fragments arranged by size: 431 364 90 AlwNI CAG_nnn'CTG Cuts at: 0 285 321 885 Size: 285 36 564 Fragments arranged by size: 564 285 36 ApaI G_GGCC'C Cuts at: 0 149 533 578 679 885 Size: 149 384 45 101 206 Fragments arranged by size: 384 206 149 101 45 AvaI C'yCGr_G Cuts at: 0 113 142 633 782 885 Size: 113 29 491 149 103 Fragments arranged by size: 491 149 113 103 29 BanI G'GyrC_C Cuts at: 0 94 885 Size: 94 791 BanII G_rGCy'C Cuts at: 0 143 149 374 533 578 679 885 Size: 143 6 225 159 45 101 206 Fragments arranged by size: 225 206 159 143 101 45 6 BbeI G_GCGC'C Cuts at: 0 98 885 Size: 98 787 BbsI GAAGACnn'nnnn_ Cuts at: 0 99 885 Size: 99 786 BglI GCCn_nnn'nGGC Cuts at: 0 67 76 118 568 885 Size: 67 9 42 450 317 Fragments arranged by size: 450 317 67 42 9 Bme1580I G_kGCm'C Cuts at: 0 149 443 533 578 679 885 Size: 149 294 90 45 101 206 Fragments arranged by size: 294 206 149 101 90 45 BmgBI CAC'GTC Cuts at: 0 584 885 Size: 584 301 BmrI ACTGGGnnnn_n' Cuts at: 0 82 885 Size: 82 803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 885 Size: 670 215 BsaAI yAC'GTr Cuts at: 0 657 885 Size: 657 228 BsaHI Gr'CG_yC Cuts at: 0 95 885 Size: 95 790 BseYI C'CCAG_C Cuts at: 0 154 321 359 672 885 Size: 154 167 38 313 213 Fragments arranged by size: 313 213 167 154 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 367 481 679 694 885 Size: 324 43 114 198 15 191 Fragments arranged by size: 324 198 191 114 43 15 BsiEI CG_ry'CG Cuts at: 0 572 885 Size: 572 313 BsiHKAI G_wGCw'C Cuts at: 0 143 374 780 885 Size: 143 231 406 105 Fragments arranged by size: 406 231 143 105 Bsp1286I G_dGCh'C Cuts at: 0 143 149 374 443 533 578 679 780 Size: 143 6 225 69 90 45 101 101 Cuts at: 780 885 Size: 105 Fragments arranged by size: 225 143 105 101 101 90 69 45 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 182 389 395 509 651 885 Size: 182 207 6 114 142 234 Fragments arranged by size: 234 207 182 142 114 6 BsrFI r'CCGG_y Cuts at: 0 792 885 Size: 792 93 BstAPI GCAn_nnn'nTGC Cuts at: 0 285 390 885 Size: 285 105 495 Fragments arranged by size: 495 285 105 BstEII G'GTnAC_C Cuts at: 0 216 549 885 Size: 216 333 336 Fragments arranged by size: 336 333 216 Bsu36I CC'TnA_GG Cuts at: 0 804 885 Size: 804 81 BtgI C'CryG_G Cuts at: 0 444 885 Size: 444 441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 136 885 Size: 136 749 BtsI GCAGTG_nn' Cuts at: 0 864 885 Size: 864 21 EaeI y'GGCC_r Cuts at: 0 59 236 272 569 608 756 885 Size: 59 177 36 297 39 148 129 Fragments arranged by size: 297 177 148 129 59 39 36 EagI C'GGCC_G Cuts at: 0 569 885 Size: 569 316 EarI CTCTTCn'nnn_ Cuts at: 0 196 205 885 Size: 196 9 680 Fragments arranged by size: 680 196 9 EcoICRI GAG'CTC Cuts at: 0 141 372 885 Size: 141 231 513 Fragments arranged by size: 513 231 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 885 Size: 670 215 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 683 885 Size: 52 631 202 Fragments arranged by size: 631 202 52 EcoO109I rG'GnC_Cy Cuts at: 0 78 146 230 575 676 885 Size: 78 68 84 345 101 209 Fragments arranged by size: 345 209 101 84 78 68 FspI TGC'GCA Cuts at: 0 430 885 Size: 430 455 HaeII r_GCGC'y Cuts at: 0 98 454 885 Size: 98 356 431 Fragments arranged by size: 431 356 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 217 249 885 Size: 217 32 636 Fragments arranged by size: 636 217 32 KasI G'GCGC_C Cuts at: 0 94 885 Size: 94 791 MslI CAynn'nnrTG Cuts at: 0 346 436 885 Size: 346 90 449 Fragments arranged by size: 449 346 90 MspA1I CmG'CkG Cuts at: 0 527 535 885 Size: 527 8 350 Fragments arranged by size: 527 350 8 NaeI GCC'GGC Cuts at: 0 794 885 Size: 794 91 NarI GG'CG_CC Cuts at: 0 95 885 Size: 95 790 NgoMIV G'CCGG_C Cuts at: 0 792 885 Size: 792 93 NspI r_CATG'y Cuts at: 0 330 885 Size: 330 555 PasI CC'CwG_GG Cuts at: 0 149 885 Size: 149 736 PfoI T'CCnGG_A Cuts at: 0 842 885 Size: 842 43 PshAI GACnn'nnGTC Cuts at: 0 133 885 Size: 133 752 PspOMI G'GGCC_C Cuts at: 0 145 529 574 675 885 Size: 145 384 45 101 210 Fragments arranged by size: 384 210 145 101 45 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 736 768 885 Size: 736 32 117 Fragments arranged by size: 736 117 32 PstI C_TGCA'G Cuts at: 0 260 395 418 885 Size: 260 135 23 467 Fragments arranged by size: 467 260 135 23 SacI G_AGCT'C Cuts at: 0 143 374 885 Size: 143 231 511 Fragments arranged by size: 511 231 143 SapI GCTCTTCn'nnn_ Cuts at: 0 196 205 885 Size: 196 9 680 Fragments arranged by size: 680 196 9 SbfI CC_TGCA'GG Cuts at: 0 260 885 Size: 260 625 SfcI C'TryA_G Cuts at: 0 256 391 414 522 885 Size: 256 135 23 108 363 Fragments arranged by size: 363 256 135 108 23 SfiI GGCCn_nnn'nGGCC Cuts at: 0 67 76 568 885 Size: 67 9 492 317 Fragments arranged by size: 492 317 67 9 SfoI GGC'GCC Cuts at: 0 96 885 Size: 96 789 SmaI CCC'GGG Cuts at: 0 115 885 Size: 115 770 SphI G_CATG'C Cuts at: 0 330 885 Size: 330 555 StyI C'CwwG_G Cuts at: 0 74 221 885 Size: 74 147 664 Fragments arranged by size: 664 147 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 829 885 Size: 829 56 TatI w'GTAC_w Cuts at: 0 665 885 Size: 665 220 XmaI C'CCGG_G Cuts at: 0 113 885 Size: 113 772 XmnI GAAnn'nnTTC Cuts at: 0 748 885 Size: 748 137 Enzymes that do cut: AarI AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstEII Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI PshAI PspOMI PsrI PstI SacI SapI SbfI SfcI SfiI SfoI SmaI SphI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI