################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvnI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI Sau3AI ScaI SexAI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 94 GGCGCC G[GA]^CG[CT]C AflIII 14 ACGTGT A^C[GA][CT]GT AocI 803 CCTCAGG CC^T[ACGT]AGG Asp700I 744 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 428 TGCGCA TGC^GCA BanI 94 GGCGCC G^G[CT][GA]CC BbeI 94 GGCGCC GGCGC^C BbsI 106 GTCTTC GAAGAC(2/6) BpmI 649 CTGGAG CTGGAG(16/14) BpuAI 106 GTCTTC GAAGAC(2/6) BsaAI 655 TACGTG [CT]AC^GT[GA] BsaHI 94 GGCGCC G[GA]^CG[CT]C BsiEI 569 CGGCCG CG[GA][CT]^CG BsmAI 282 GTCTC GTCTC(1/5) BsrFI 792 GCCGGC [GA]^CCGG[CT] Bsu36I 803 CCTCAGG CC^T[ACGT]AGG Cfr10I 792 GCCGGC [GA]^CCGG[CT] DsaI 444 CCACGG C^C[GA][CT]GG EagI 569 CGGCCG C^GGCCG EclXI 569 CGGCCG C^GGCCG Eco52I 569 CGGCCG C^GGCCG Eco81I 803 CCTCAGG CC^T[ACGT]AGG FspI 428 TGCGCA TGC^GCA HgaI 129 GACGC GACGC(5/10) Hin1I 94 GGCGCC G[GA]^CG[CT]C KasI 94 GGCGCC G^GCGCC MseI 11 TTAA T^TAA NaeI 792 GCCGGC GCC^GGC NarI 94 GGCGCC GG^CGCC NgoMIV 792 GCCGGC G^CCGGC NspI 326 GCATGC [GA]CATG^[CT] PshAI 129 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 255 CCTGCAGG CCTGCA^GG SfaNI 348 GATGC GCATC(5/9) SfoI 94 GGCGCC GGC^GCC SmaI 113 CCCGGG CCC^GGG SphI 326 GCATGC GCATG^C Sse8387I 255 CCTGCAGG CCTGCA^GG TaqI 137 TCGA T^CGA Tru9I 11 TTAA T^TAA TthHB8I 137 TCGA T^CGA XmaCI 113 CCCGGG C^CCGGG XmaI 113 CCCGGG C^CCGGG XmnI 744 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 280 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 316 CAGGGGCTG AvaII 693 GGTCC G^G[AT]CC 833 GGTCC BstAPI 279 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 384 GCACCTGCTGC BstEII 216 GGTCACC G^GT[ACGT]ACC 549 GGTCACC BstF5I 434 CATCC GGATG(2/0) 689 GGATG BstPI 216 GGTCACC G^GT[ACGT]ACC 549 GGTCACC DdeI 373 CTCAG C^T[ACGT]AG 804 CTCAG EarI 190 CTCTTC CTCTTC(1/4) 199 CTCTTC EcoNI 48 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 679 CCTCTGACAGG EcoO65I 216 GGTCACC G^GT[ACGT]ACC 549 GGTCACC EcoT14I 74 CCAAGG C^C[AT][AT]GG 221 CCTTGG FokI 434 CATCC GGATG(9/13) 689 GGATG HaeII 94 GGCGCC [GA]GCGC^[CT] 450 GGCGCT Ksp632I 190 CTCTTC CTCTTC(1/4) 199 CTCTTC MslI 342 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 432 CACATCCGTG MspA1I 525 CAGCGG C[AC]G^C[GT]G 533 CCGCTG SacI 139 GAGCTC GAGCT^C 370 GAGCTC SapI 189 GCTCTTC GCTCTTC(1/4) 198 GCTCTTC StyI 74 CCAAGG C^C[AT][AT]GG 221 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI ApaI AspHI AvaI BanII BbvI BcnI BglI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfcI SfiI TseI Tsp45I TspRI