DDX53 ORF clone pFN21AE5791

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5791

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Clone info.

Species

human

Clone Name

pFN21AE5791 Sequence (with vector) Restriction Map

Product ID

FHC29424

ORF Sequence

DNA sequence (1893 bp)
Protein sequence (631 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5791

Symbol

DDX53

Alias : CAGE, CT26

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 53

Original Clone ID

cp95791

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1893 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 631 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35214.1 0 100.0 DDX53 CCDS4977.1 1.9e-148 61.8 DDX43 CCDS33646.1 5.8e-64 43.6 DDX17 CCDS46706.1 5.8e-64 43.6 DDX17 CCDS82190.1 6.3e-63 41.8 DDX5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018111 53 109 PF00013 K Homology IPR011545 246 416 PF00270 DNA/RNA helicase IPR001650 488 562 PF00271 Helicase HMMSmart IPR004087 47 114 SM00322 K Homology IPR014001 241 444 SM00487 DEAD-like helicase IPR001650 481 562 SM00490 Helicase ProfileScan IPR004088 48 109 PS50084 K Homology IPR014014 222 250 PS51195 RNA helicase IPR014001 253 428 PS51192 DEAD-like helicase IPR001650 440 601 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061135 648 606286 61.8 98.9 XP_011534230 529 606286 64.9 83.7 XP_011534229 529 606286 64.9 83.7 XP_011534228 604 606286 59.4 98.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5791 Download

>KIEE5791  1893 bp
ATGTCCCACTGGGCCCCAGAGTGGAAGAGGGCGGAGGCTAATCCAAGAGACCTTGGGGCC
AGCTGGGATGTCAGGGGCAGCAGAGGCAGTGGCTGGAGTGGCCCCTTCGGCCATCAGGGA
CCGAGAGCAGCAGGCTCCCGTGAACCACCACTCTGCTTTAAAATAAAGAACAATATGGTT
GGTGTGGTCATTGGTTACAGTGGATCAAAAATAAAAGATCTACAACATTCGACAAACACT
AAAATACAGATCATAAACGGGGAATCTGAAGCAAAAGTCAGAATTTTTGGCAATAGGGAA
ATGAAAGCAAAGGCCAAAGCGGCTATAGAAACACTTATTAGAAAACAAGAAAGCTACAAC
TCAGAATCCAGTGTGGATAATGCTGCATCCCAAACCCCTATTGGAAGAAATCTAGGCAGA
AATGACATTGTTGGAGAAGCTGAGCCATTGTCAAATTGGGATCGCATTAGGGCAGCAGTC
GTGGAGTGCGAAAAGAGAAAATGGGCAGATCTACCACCAGTTAAGAAAAACTTTTACATA
GAATCCAAAGCAACAAGCTGCATGTCTGAAATGCAGGTGATTAACTGGAGAAAGGAAAAT
TTCAACATAACGTGTGATGACTTGAAAAGTGGTGAAAAGCGTCTCATTCCAAAACCAACT
TGCAGGTTTAAAGACGCTTTTCAGCAATACCCTGATCTTCTGAAAAGCATAATAAGGGTA
GGGATTGTAAAGCCAACGCCAATTCAGTCACAGGCATGGCCAATTATTCTACAAGGAATA
GATCTTATAGTAGTTGCACAAACCGGAACAGGGAAAACATTGTCCTATCTAATGCCTGGG
TTTATTCATCTTGATTCTCAACCAATATCTAGAGAGCAAAGGAATGGGCCTGGGATGCTA
GTCCTTACACCCACTAGAGAGTTGGCTCTTCACGTGGAAGCTGAATGTTCAAAGTATTCA
TATAAAGGTCTCAAAAGCATTTGCATATATGGTGGTAGAAACAGAAATGGACAAATAGAA
GACATTAGCAAAGGTGTAGATATCATTATTGCAACTCCTGGGAGGCTGAATGACCTACAA
ATGAATAACTCTGTCAACCTAAGAAGCATAACCTACTTGGTTATAGATGAGGCAGATAAA
ATGCTGGATATGGAATTTGAACCCCAGATAAGGAAGATTTTATTAGATGTGCGCCCAGAC
CGACAGACTGTTATGACAAGTGCAACTTGGCCAGATACTGTACGTCAACTAGCACTTTCT
TATTTGAAAGATCCTATGATTGTTTATGTTGGTAATCTGAATCTAGTGGCTGTAAATACA
GTGAAGCAAAATATAATTGTTACCACAGAAAAAGAAAAACGAGCTCTCACCCAAGAATTC
GTAGAGAACATGTCACCCAACGACAAAGTCATCATGTTTGTCAGCCAAAAACATATTGCT
GATGACTTGTCAAGCGACTTCAATATCCAAGGCATATCTGCAGAATCATTACATGGCAAC
AGTGAACAGAGTGATCAAGAGCGAGCAGTAGAGGACTTTAAAAGCGGAAACATAAAGATA
CTGATTACAACTGATATAGTATCCCGAGGTCTTGATCTTAATGATGTCACACATGTATAT
AATTATGATTTCCCAAGGAATATTGACGTATATGTACACAGAGTAGGGTACATTGGACGG
ACAGGAAAGACTGGCACATCAGTTACCCTCATCACTCAGAGAGATTCGAAAATGGCCGGT
GAATTGATTAAAATTCTGGACAGAGCAAATCAGAGTGTTCCGGAAGATCTTGTAGTAATG
GCTGAGCAATACAAGTTAAATCAACAAAAGAGGCACAGAGAAACACGATCAAGAAAACCT
GGACAAAGACGCAAGGAGTTTTATTTTTTAAGT

ORF Protein

Cloned ORF protein sequence for pFN21AE5791 Download

>KIEE5791  631 aa
MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMV
GVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYN
SESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYI
ESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRV
GIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGML
VLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQ
MNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALS
YLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIA
DDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVY
NYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVM
AEQYKLNQQKRHRETRSRKPGQRRKEFYFLS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5791 Download

>pFN21AE5791  6598 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCCACTGGGCCCCAGAGTGGAAGAGGGCGGA
GGCTAATCCAAGAGACCTTGGGGCCAGCTGGGATGTCAGGGGCAGCAGAGGCAGTGGCTG
GAGTGGCCCCTTCGGCCATCAGGGACCGAGAGCAGCAGGCTCCCGTGAACCACCACTCTG
CTTTAAAATAAAGAACAATATGGTTGGTGTGGTCATTGGTTACAGTGGATCAAAAATAAA
AGATCTACAACATTCGACAAACACTAAAATACAGATCATAAACGGGGAATCTGAAGCAAA
AGTCAGAATTTTTGGCAATAGGGAAATGAAAGCAAAGGCCAAAGCGGCTATAGAAACACT
TATTAGAAAACAAGAAAGCTACAACTCAGAATCCAGTGTGGATAATGCTGCATCCCAAAC
CCCTATTGGAAGAAATCTAGGCAGAAATGACATTGTTGGAGAAGCTGAGCCATTGTCAAA
TTGGGATCGCATTAGGGCAGCAGTCGTGGAGTGCGAAAAGAGAAAATGGGCAGATCTACC
ACCAGTTAAGAAAAACTTTTACATAGAATCCAAAGCAACAAGCTGCATGTCTGAAATGCA
GGTGATTAACTGGAGAAAGGAAAATTTCAACATAACGTGTGATGACTTGAAAAGTGGTGA
AAAGCGTCTCATTCCAAAACCAACTTGCAGGTTTAAAGACGCTTTTCAGCAATACCCTGA
TCTTCTGAAAAGCATAATAAGGGTAGGGATTGTAAAGCCAACGCCAATTCAGTCACAGGC
ATGGCCAATTATTCTACAAGGAATAGATCTTATAGTAGTTGCACAAACCGGAACAGGGAA
AACATTGTCCTATCTAATGCCTGGGTTTATTCATCTTGATTCTCAACCAATATCTAGAGA
GCAAAGGAATGGGCCTGGGATGCTAGTCCTTACACCCACTAGAGAGTTGGCTCTTCACGT
GGAAGCTGAATGTTCAAAGTATTCATATAAAGGTCTCAAAAGCATTTGCATATATGGTGG
TAGAAACAGAAATGGACAAATAGAAGACATTAGCAAAGGTGTAGATATCATTATTGCAAC
TCCTGGGAGGCTGAATGACCTACAAATGAATAACTCTGTCAACCTAAGAAGCATAACCTA
CTTGGTTATAGATGAGGCAGATAAAATGCTGGATATGGAATTTGAACCCCAGATAAGGAA
GATTTTATTAGATGTGCGCCCAGACCGACAGACTGTTATGACAAGTGCAACTTGGCCAGA
TACTGTACGTCAACTAGCACTTTCTTATTTGAAAGATCCTATGATTGTTTATGTTGGTAA
TCTGAATCTAGTGGCTGTAAATACAGTGAAGCAAAATATAATTGTTACCACAGAAAAAGA
AAAACGAGCTCTCACCCAAGAATTCGTAGAGAACATGTCACCCAACGACAAAGTCATCAT
GTTTGTCAGCCAAAAACATATTGCTGATGACTTGTCAAGCGACTTCAATATCCAAGGCAT
ATCTGCAGAATCATTACATGGCAACAGTGAACAGAGTGATCAAGAGCGAGCAGTAGAGGA
CTTTAAAAGCGGAAACATAAAGATACTGATTACAACTGATATAGTATCCCGAGGTCTTGA
TCTTAATGATGTCACACATGTATATAATTATGATTTCCCAAGGAATATTGACGTATATGT
ACACAGAGTAGGGTACATTGGACGGACAGGAAAGACTGGCACATCAGTTACCCTCATCAC
TCAGAGAGATTCGAAAATGGCCGGTGAATTGATTAAAATTCTGGACAGAGCAAATCAGAG
TGTTCCGGAAGATCTTGTAGTAATGGCTGAGCAATACAAGTTAAATCAACAAAAGAGGCA
CAGAGAAACACGATCAAGAAAACCTGGACAAAGACGCAAGGAGTTTTATTTTTTAAGTGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT