(Circular) (Six-base) MAPSORT of: pFN21AE5791.seq Check: 9501 from: 1 to: 6598 pFN21AE5791 6598 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2569 2569 Size: 6598 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3941 1937 Size: 2004 4594 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 4661 6045 6313 1632 Size: 660 2369 1384 268 1917 Fragments arranged by size: 2369 1917 1384 660 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 2615 3393 3616 5497 6474 1203 Size: 1412 778 223 1881 977 1327 Fragments arranged by size: 1881 1412 1327 977 778 223 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2578 1184 Size: 1394 5204 AlwNI CAG_nnn'CTG Cuts at: 2097 3242 4341 5913 2097 Size: 1145 1099 1572 2782 Fragments arranged by size: 2782 1572 1145 1099 ApaI G_GGCC'C Cuts at: 2020 2020 Size: 6598 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 2286 2602 3158 3380 3756 3907 4133 4285 Size: 793 316 556 222 376 151 226 152 Cuts at: 4285 6394 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 793 556 376 316 226 222 152 151 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 3588 3923 1870 Size: 73 1645 335 4545 Fragments arranged by size: 4545 1645 335 73 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3919 5374 618 Size: 325 217 627 2132 1455 1842 Fragments arranged by size: 2132 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 3370 3917 1391 Size: 629 1350 547 4072 Fragments arranged by size: 4072 1350 629 547 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3030 928 Size: 971 1131 4496 Fragments arranged by size: 4496 1131 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3595 4490 5700 3595 Size: 895 1210 4493 Fragments arranged by size: 4493 1210 895 BclI T'GATC_A Cuts at: 1548 3517 1548 Size: 1969 4629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 2324 5209 Size: 107 122 71 631 605 440 212 2885 Cuts at: 5209 136 Size: 1525 Fragments arranged by size: 2885 1525 631 605 440 212 122 107 71 BglII A'GATC_T Cuts at: 2221 2512 2785 3790 6593 2221 Size: 291 273 1005 2803 2226 Fragments arranged by size: 2803 2226 1005 291 273 BlpI GC'TnA_GC Cuts at: 2445 3807 4015 2445 Size: 1362 208 5028 Fragments arranged by size: 5028 1362 208 Bme1580I G_kGCm'C Cuts at: 1790 2020 4659 5815 1790 Size: 230 2639 1156 2573 Fragments arranged by size: 2639 2573 1156 230 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 4329 5287 Size: 416 53 83 399 24 606 2306 958 Cuts at: 5287 442 Size: 1753 Fragments arranged by size: 2306 1753 958 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2611 5258 1239 Size: 880 492 2647 2579 Fragments arranged by size: 2647 2579 880 492 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4723 5588 5886 4723 Size: 865 298 5435 Fragments arranged by size: 5435 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 2978 5261 882 Size: 617 547 932 2283 2219 Fragments arranged by size: 2283 2219 932 617 547 BsaAI yAC'GTr Cuts at: 493 1204 2938 4389 493 Size: 711 1734 1451 2702 Fragments arranged by size: 2702 1734 1451 711 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2807 3784 5031 5429 5703 5850 6261 2807 Size: 977 1247 398 274 147 411 3144 Fragments arranged by size: 3144 1247 977 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6373 Fragments arranged by size: 6373 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6598 BseYI C'CCAG_C Cuts at: 2067 5801 6297 2067 Size: 3734 496 2368 Fragments arranged by size: 3734 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6025 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4809 5837 1540 Size: 275 186 2047 761 1028 2301 Fragments arranged by size: 2301 2047 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3370 4659 4744 5815 3370 Size: 1289 85 1071 4153 Fragments arranged by size: 4153 1289 1071 85 BsmI GAATG_Cn' Cuts at: 4109 4202 4109 Size: 93 6505 BsmBI CGTCTCn'nnnn_ Cuts at: 2651 2651 Size: 6598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 3370 3917 4659 4744 5815 1391 Size: 399 230 1350 547 742 85 1071 2174 Fragments arranged by size: 2174 1350 1071 742 547 399 230 85 BspEI T'CCGG_A Cuts at: 3784 6261 3784 Size: 2477 4121 BspHI T'CATG_A Cuts at: 4487 6217 4487 Size: 1730 4868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 2658 3953 844 Size: 1725 89 1295 3489 Fragments arranged by size: 3489 1725 1295 89 BsrBI CCG'CTC Cuts at: 1394 1823 4485 1394 Size: 429 2662 3507 Fragments arranged by size: 3507 2662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3740 5242 5429 1700 Size: 110 1930 1502 187 2869 Fragments arranged by size: 2869 1930 1502 187 110 BsrGI T'GTAC_A Cuts at: 96 3658 96 Size: 3562 3036 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 4658 5670 4658 Size: 1012 5586 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6598 BstBI TT'CG_AA Cuts at: 3731 5402 3731 Size: 1671 4927 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstYI r'GATC_y Cuts at: 1972 2221 2512 2785 3274 3790 3928 4295 4680 Size: 249 291 273 489 516 138 367 385 Cuts at: 4680 4697 6149 6235 6247 6593 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 489 385 367 346 291 273 249 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6046 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5859 Fragments arranged by size: 5859 683 56 BtsI GCAGTG_nn' Cuts at: 2098 4114 4908 4928 2098 Size: 2016 794 20 3768 Fragments arranged by size: 3768 2016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 2164 2674 3544 3902 2164 Size: 510 870 358 4860 Fragments arranged by size: 4860 870 510 358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2762 3233 Size: 54 1425 50 275 18 283 649 471 Cuts at: 3233 3738 4045 4934 6563 8 Size: 505 307 889 1629 43 Fragments arranged by size: 1629 1425 889 649 505 471 307 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2937 4528 1353 Size: 671 913 1591 3423 Fragments arranged by size: 3423 1591 913 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 5185 5559 5705 387 Size: 1064 138 462 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 462 374 146 138 EcoICRI GAG'CTC Cuts at: 3368 3368 Size: 6598 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2292 2611 4661 5258 6045 6313 Size: 393 487 173 319 2050 597 787 268 Cuts at: 6313 1239 Size: 1524 Fragments arranged by size: 2050 1524 787 597 487 393 319 268 173 EcoO109I rG'GnC_Cy Cuts at: 2017 2017 Size: 6598 EcoRI G'AATT_C Cuts at: 3380 3907 4285 3380 Size: 527 378 5693 Fragments arranged by size: 5693 527 378 EcoRV GAT'ATC Cuts at: 3046 3046 Size: 6598 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2551 3246 2551 Size: 695 5903 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 5745 1678 Size: 4067 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 3380 3412 3437 Size: 32 135 32 866 32 910 32 25 Cuts at: 3437 3469 3569 3601 5239 5271 5313 5345 1373 Size: 32 100 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 910 866 135 100 42 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3100 3251 3942 4188 669 Size: 988 281 1162 151 691 246 3079 Fragments arranged by size: 3079 1162 988 691 281 246 151 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2416 5712 5896 1305 Size: 1111 3296 184 2007 Fragments arranged by size: 3296 2007 1111 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2764 3235 10 Size: 54 1425 343 932 471 3373 Fragments arranged by size: 3373 1425 932 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2758 4556 4915 5074 518 Size: 648 1592 1798 359 159 2042 Fragments arranged by size: 2042 1798 1592 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 4014 4691 5839 6084 1606 Size: 461 1947 677 1148 245 2120 Fragments arranged by size: 2120 1947 1148 677 461 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6046 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2569 3397 3620 3956 5501 1132 Size: 97 1340 828 223 336 1545 2229 Fragments arranged by size: 2229 1545 1340 828 336 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 3393 3616 5497 3393 Size: 223 1881 4494 Fragments arranged by size: 4494 1881 223 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PmlI CAC'GTG Cuts at: 2938 2938 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2139 2171 4667 4699 6180 6212 2139 Size: 32 2496 32 1481 32 2525 Fragments arranged by size: 2525 2496 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 2016 2016 Size: 6598 PstI C_TGCA'G Cuts at: 830 3487 3950 830 Size: 2657 463 3478 Fragments arranged by size: 3478 2657 463 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2067 2067 Size: 6598 SacI G_AGCT'C Cuts at: 3370 3370 Size: 6598 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2937 2937 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SfcI C'TryA_G Cuts at: 826 1045 2328 3483 3946 5081 5762 5953 826 Size: 219 1283 1155 463 1135 681 191 1471 Fragments arranged by size: 1471 1283 1155 1135 681 463 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2112 2112 Size: 6598 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3925 1872 Size: 2053 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4702 5603 5865 820 Size: 197 926 2759 901 262 1553 Fragments arranged by size: 2759 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SspI AAT'ATT Cuts at: 5 52 3646 4522 5 Size: 47 3594 876 2081 Fragments arranged by size: 3594 2081 876 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 2056 3472 3638 4037 513 Size: 552 991 1416 166 399 3074 Fragments arranged by size: 3074 1416 991 552 399 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 2929 3219 3389 3415 4625 4642 Size: 1255 560 789 290 170 26 1210 17 Cuts at: 4642 4795 4980 5425 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1210 789 560 445 290 185 170 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3658 4844 6556 Size: 274 80 33 51 1443 1681 1186 1712 Cuts at: 6556 96 Size: 138 Fragments arranged by size: 1712 1681 1443 1186 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6558 6590 6558 Size: 32 6566 Tth111I GACn'n_nGTC Cuts at: 1316 3410 1316 Size: 2094 4504 XbaI T'CTAG_A Cuts at: 2873 3934 2873 Size: 1061 5537 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2457 2923 2402 Size: 55 466 6077 Fragments arranged by size: 6077 466 55 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 3923 1870 Size: 2053 4545 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI