Restriction map for
pFN21AE5791
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BcnI BfrI BlnI BsaBI
BsaHI BseRI BsgI BsiEI BsiWI
BsmI BspDI BspHI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FauI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1779 TCCGGA T^CCGGA
ApaI 11 GGGCCC GGGCC^C
AspHI 1361 GAGCTC G[AT]GC[AT]^C
AspI 1402 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1583 CCCGAG C^[CT]CG[GA]G
AvaII 118 GGACC G^G[AT]CC
BbrPI 931 CACGTG CAC^GTG
BbsI 1018 GAAGAC GAAGAC(2/6)
BciVI 1579 GTATCC GTATCC(6/5)
BclI 1512 TGATCA T^GATCA
BpuAI 1018 GAAGAC GAAGAC(2/6)
BsaAI 931 CACGTG [CT]AC^GT[GA]
BseAI 1779 TCCGGA T^CCGGA
BsiHKAI 1361 GAGCTC G[AT]GC[AT]^C
BsmBI 640 CGTCTC CGTCTC(1/5)
BsoBI 1583 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1653 TGTACA T^GTACA
BspEI 1779 TCCGGA T^CCGGA
BsrFI 1735 GCCGGT [GA]^CCGG[CT]
BsrGI 1653 TGTACA T^GTACA
BstBI 1725 TTCGAA TT^CGAA
CfoI 1191 GCGC GCG^C
Cfr10I 1735 GCCGGT [GA]^CCGG[CT]
DraII 11 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 11 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1375 GAATTC G^AATTC
EcoRV 1039 GATATC GAT^ATC
FbaI 1512 TGATCA T^GATCA
HhaI 1191 GCGC GCG^C
HinP1I 1191 GCGC G^CGC
MroI 1779 TCCGGA T^CCGGA
MslI 749 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 60 CAGCTG C[AC]G^C[GT]G
NspV 1725 TTCGAA TT^CGAA
PflFI 1402 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 931 CACGTG CAC^GTG
PmlI 931 CACGTG CAC^GTG
PstI 1478 CTGCAG CTGCA^G
PvuII 60 CAGCTG CAG^CTG
SacI 1361 GAGCTC GAGCT^C
SapI 925 GCTCTTC GCTCTTC(1/4)
SfiI 100 GGCCCCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1725 TTCGAA TT^CGAA
SspBI 1653 TGTACA T^GTACA
SspI 1639 AATATT AAT^ATT
Tth111I 1402 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 868 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 87 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1232 CAGATACTG
BalI 757 TGGCCA TGG^CCA
1228 TGGCCA
BanII 11 GGGCCC G[GA]GC[CT]^C
1361 GAGCTC
BglI 101 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
313 GCCAAAGCGGC
BlpI 439 GCTGAGC GC^T[ACGT]AGC
1801 GCTGAGC
BmyI 11 GGGCCC G[AGT]GC[ACT]^C
1361 GAGCTC
BpmI 93 CTGGAG CTGGAG(16/14)
585 CTGGAG
Bpu1102I 439 GCTGAGC GC^T[ACGT]AGC
1801 GCTGAGC
BsaI 47 GAGACC GGTCTC(1/5)
967 GGTCTC
BsaWI 802 ACCGGA [AT]^CCGG[AT]
1779 TCCGGA
BsmFI 117 GGGAC GGGAC(10/14)
3 GTCCC
Bsp1286I 11 GGGCCC G[AGT]GC[ACT]^C
1361 GAGCTC
BspLU11I 1388 ACATGT A^CATGT
1611 ACATGT
BspMI 573 GCAGGT ACCTGC(4/8)
662 GCAGGT
CelII 439 GCTGAGC GC^T[ACGT]AGC
1801 GCTGAGC
EarI 926 CTCTTC CTCTTC(1/4)
24 GAAGAG
HincII 1093 GTCAAC GT[CT]^[GA]AC
1244 GTCAAC
HindII 1093 GTCAAC GT[CT]^[GA]AC
1244 GTCAAC
Ksp632I 926 CTCTTC CTCTTC(1/4)
24 GAAGAG
MluNI 757 TGGCCA TGG^CCA
1228 TGGCCA
MscI 757 TGGCCA TGG^CCA
1228 TGGCCA
SfaNI 894 GATGC GCATC(5/9)
385 GCATC
SfcI 323 CTATAG C^T[GA][CT]AG
1478 CTGCAG
TaqI 229 TCGA T^CGA
1726 TCGA
TthHB8I 229 TCGA T^CGA
1726 TCGA
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbvI BfaI BglII
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII