Restriction map for
pFN21AE5791
(Linear) (Six-base) MAPSORT of: KIEE5791.seq Check: 4741 from: 1 to: 1893
KIEE5791.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 564 1893
Size: 564 1329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1893
Size: 287 1606
AflIII A'CryG_T
Cuts at: 0 610 1388 1611 1893
Size: 610 778 223 282
Fragments arranged by size:
778 610 282 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 573 1893
Size: 573 1320
AlwNI CAG_nnn'CTG
Cuts at: 0 92 1237 1893
Size: 92 1145 656
Fragments arranged by size:
1145 656 92
ApaI G_GGCC'C
Cuts at: 0 15 1893
Size: 15 1878
ApoI r'AATT_y
Cuts at: 0 281 597 1153 1375 1751 1893
Size: 281 316 556 222 376 142
Fragments arranged by size:
556 376 316 281 222 142
AvaI C'yCGr_G
Cuts at: 0 1583 1893
Size: 1583 310
BanII G_rGCy'C
Cuts at: 0 15 1365 1893
Size: 15 1350 528
Fragments arranged by size:
1350 528 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1025 1893
Size: 1025 868
BciVI GTATCCnnnnn_n'
Cuts at: 0 1590 1893
Size: 1590 303
BclI T'GATC_A
Cuts at: 0 1512 1893
Size: 1512 381
BglI GCCn_nnn'nGGC
Cuts at: 0 107 319 1893
Size: 107 212 1574
Fragments arranged by size:
1574 212 107
BglII A'GATC_T
Cuts at: 0 216 507 780 1785 1893
Size: 216 291 273 1005 108
Fragments arranged by size:
1005 291 273 216 108
BlpI GC'TnA_GC
Cuts at: 0 440 1802 1893
Size: 440 1362 91
Fragments arranged by size:
1362 440 91
Bme1580I G_kGCm'C
Cuts at: 0 15 1893
Size: 15 1878
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 1893
Size: 18 1875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 606 1893
Size: 114 492 1287
Fragments arranged by size:
1287 492 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 41 973 1893
Size: 41 932 920
Fragments arranged by size:
932 920 41
BsaAI yAC'GTr
Cuts at: 0 933 1893
Size: 933 960
BsaWI w'CCGG_w
Cuts at: 0 802 1779 1893
Size: 802 977 114
Fragments arranged by size:
977 802 114
BseYI C'CCAG_C
Cuts at: 0 62 1893
Size: 62 1831
BsiHKAI G_wGCw'C
Cuts at: 0 1365 1893
Size: 1365 528
BsmBI CGTCTCn'nnnn_
Cuts at: 0 646 1893
Size: 646 1247
Bsp1286I G_dGCh'C
Cuts at: 0 15 1365 1893
Size: 15 1350 528
Fragments arranged by size:
1350 528 15
BspEI T'CCGG_A
Cuts at: 0 1779 1893
Size: 1779 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 653 1893
Size: 564 89 1240
Fragments arranged by size:
1240 564 89
BsrFI r'CCGG_y
Cuts at: 0 1735 1893
Size: 1735 158
BsrGI T'GTAC_A
Cuts at: 0 1653 1893
Size: 1653 240
BstBI TT'CG_AA
Cuts at: 0 1726 1893
Size: 1726 167
BstYI r'GATC_y
Cuts at: 0 216 507 780 1269 1785 1893
Size: 216 291 273 489 516 108
Fragments arranged by size:
516 489 291 273 216 108
BtsI GCAGTG_nn'
Cuts at: 0 93 1893
Size: 93 1800
DraI TTT'AAA
Cuts at: 0 159 669 1539 1893
Size: 159 510 870 354
Fragments arranged by size:
870 510 354 159
EaeI y'GGCC_r
Cuts at: 0 108 757 1228 1733 1893
Size: 108 649 471 505 160
Fragments arranged by size:
649 505 471 160 108
EarI CTCTTCn'nnn_
Cuts at: 0 19 932 1893
Size: 19 913 961
Fragments arranged by size:
961 913 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 1893
Size: 46 1847
EcoICRI GAG'CTC
Cuts at: 0 1363 1893
Size: 1363 530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 287 606 1893
Size: 114 173 319 1287
Fragments arranged by size:
1287 319 173 114
EcoO109I rG'GnC_Cy
Cuts at: 0 12 1893
Size: 12 1881
EcoRI G'AATT_C
Cuts at: 0 1375 1893
Size: 1375 518
EcoRV GAT'ATC
Cuts at: 0 1041 1893
Size: 1041 852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 546 1241 1893
Size: 546 695 652
Fragments arranged by size:
695 652 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1375 1407 1432 1464 1564 1596
Size: 433 32 910 32 25 32 100 32
Cuts at: 1596 1893
Size: 297
Fragments arranged by size:
910 433 297 100 32 32 32 32
25
HincII GTy'rAC
Cuts at: 0 1095 1246 1893
Size: 1095 151 647
Fragments arranged by size:
1095 647 151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1893
Size: 411 1482
MscI TGG'CCA
Cuts at: 0 759 1230 1893
Size: 759 471 663
Fragments arranged by size:
759 663 471
MslI CAynn'nnrTG
Cuts at: 0 753 1893
Size: 753 1140
MspA1I CmG'CkG
Cuts at: 0 62 1893
Size: 62 1831
NspI r_CATG'y
Cuts at: 0 564 1392 1615 1893
Size: 564 828 223 278
Fragments arranged by size:
828 564 278 223
PciI A'CATG_T
Cuts at: 0 1388 1611 1893
Size: 1388 223 282
Fragments arranged by size:
1388 282 223
PmlI CAC'GTG
Cuts at: 0 933 1893
Size: 933 960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 1893
Size: 134 32 1727
Fragments arranged by size:
1727 134 32
PspOMI G'GGCC_C
Cuts at: 0 11 1893
Size: 11 1882
PstI C_TGCA'G
Cuts at: 0 1482 1893
Size: 1482 411
PvuII CAG'CTG
Cuts at: 0 62 1893
Size: 62 1831
SacI G_AGCT'C
Cuts at: 0 1365 1893
Size: 1365 528
SapI GCTCTTCn'nnn_
Cuts at: 0 932 1893
Size: 932 961
SfcI C'TryA_G
Cuts at: 0 323 1478 1893
Size: 323 1155 415
Fragments arranged by size:
1155 415 323
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 107 1893
Size: 107 1786
SspI AAT'ATT
Cuts at: 0 1641 1893
Size: 1641 252
StyI C'CwwG_G
Cuts at: 0 51 1467 1633 1893
Size: 51 1416 166 260
Fragments arranged by size:
1416 260 166 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 924 1214 1384 1410 1893
Size: 135 789 290 170 26 483
Fragments arranged by size:
789 483 290 170 135 26
TatI w'GTAC_w
Cuts at: 0 1653 1893
Size: 1653 240
Tth111I GACn'n_nGTC
Cuts at: 0 1405 1893
Size: 1405 488
XbaI T'CTAG_A
Cuts at: 0 868 1893
Size: 868 1025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 452 918 1893
Size: 397 55 466 975
Fragments arranged by size:
975 466 397 55
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI
BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BpmI BsaI BsaAI BsaWI BseYI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrFI BsrGI BstBI BstYI BtsI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I HincII MmeI MscI MslI MspA1I
NspI PciI PmlI PpiI PspOMI PstI PvuII SacI
SapI SfcI SfiI SspI StyI TaqII TatI Tth111I
XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BfrBI BmgBI BmtI
BplI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsgI
BsiEI BsiWI BsmI BspHI BsrBI BsrDI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EcoNI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TstI XhoI XmaI
XmnI ZraI