DDX49 ORF clone pFN21AE5626

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5626

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Clone info.

Species

human

Clone Name

pFN21AE5626 Sequence (with vector) Restriction Map

Product ID

FHC26549

ORF Sequence

DNA sequence (1449 bp)
Protein sequence (483 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5626

Symbol

DDX49

Alias : Dbp8, R27090_2

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 49, transcript variant 1

Original Clone ID

oc95626

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1449 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 483 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12390.1 3.4e-169 100.0 DDX49 CCDS8655.1 4.6e-62 44.6 DDX47 CCDS13416.1 1.3e-42 33.0 DDX27 CCDS31907.1 7.9e-36 37.8 DDX54 CCDS44984.1 7.9e-36 37.8 DDX54 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 26 195 PF00270 DNA/RNA helicase IPR001650 267 343 PF00271 Helicase HMMSmart IPR014001 21 222 SM00487 DEAD-like helicase IPR001650 262 343 SM00490 Helicase ProfileScan IPR014014 2 30 PS51195 RNA helicase IPR014001 33 207 PS51192 DEAD-like helicase IPR001650 218 382 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057439 455 615428 44.6 90.9 NP_060365 796 616621 33.0 98.8 NP_957518 406 615428 40.8 90.9 NP_004389 875 601235 30.2 96.7 NP_001091974 731 608469 32.3 93.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5626 Download

>KIEE5626  1449 bp
ATGGCAGGCTTCGCGGAGCTCGGGCTGTCATCGTGGCTCGTGGAACAATGTCGGCAGCTG
GGTTTGAAGCAGCCCACGCCCGTGCAGCTCGGCTGCATCCCCGCCATCCTGGAGGGTCGA
GACTGCTTGGGCTGTGCTAAGACAGGCAGTGGGAAGACAGCAGCGTTTGTCCTTCCCATC
TTGCAGAAGCTGTCTGAGGATCCCTATGGCATCTTCTGCCTCGTCCTGACACCCACCAGG
GAGCTGGCCTACCAGATCGCAGAGCAGTTCCGGGTCCTGGGGAAGCCTCTAGGGCTGAAA
GACTGCATCATCGTCGGTGGCATGGACATGGTGGCCCAGGCGCTGGAGCTCTCTCGGAAA
CCACACGTGGTCATCGCCACGCCGGGGCGCCTGGCAGATCACCTGCGCAGCTCCAACACT
TTTAGTATAAAGAAGATCCGCTTCCTGGTGATGGATGAGGCAGACCGGCTGCTGGAACAG
GGCTGCACTGACTTCACCGTGGACCTGGAGGCCATCCTGGCGGCTGTGCCGGCCCGCAGG
CAGACACTGCTGTTCAGCGCCACGCTGACCGACACACTCCGGGAGCTGCAGGGTCTGGCC
ACCAACCAGCCCTTCTTCTGGGAAGCACAGGCCCCGGTGAGCACCGTGGAGCAGCTGGAC
CAGCGCTACCTGCTGGTGCCTGAGAAGGTCAAGGACGCCTACCTGGTCCACCTGATCCAG
CGCTTCCAGGATGAGCACGAGGACTGGTCCATTATCATCTTCACCAACACGTGCAAGACC
TGCCAGATTCTGTGCATGATGCTGCGCAAATTCAGCTTCCCCACCGTGGCTCTGCACTCC
ATGATGAAGCAGAAAGAACGCTTTGCCGCCCTAGCCAAGTTCAAGTCCAGCATCTACCGG
ATCCTGATCGCAACAGACGTGGCCTCCCGGGGCCTGGACATCCCTACGGTACAGGTGGTC
ATCAACCACAACACCCCCGGGCTCCCCAAGATCTACATCCACCGAGTCGGCCGGACGGCC
CGTGCAGGGCGGCAGGGTCAGGCCATCACGCTGGTGACACAGTACGACATCCACCTGGTG
CACGCCATCGAGGAGCAGATCAAGAAGAAGCTGGAGGAGTTCTCCGTGGAAGAGGCCGAG
GTGCTACAGATCCTCACACAGGTCAACGTGGTGCGAAGAGAGTGTGAGATCAAACTGGAG
GCGGCCCACTTTGACGAAAAGAAGGAGATCAACAAACGGAAGCAGCTGATCCTGGAGGGG
AAGGACCCTGACCTGGAGGCCAAGCGCAAGGCTGAGCTGGCCAAGATCAAGCAGAAGAAC
CGGCGCTTCAAGGAGAAGGTGGAGGAGACGCTGAAGCGACAGAAGGCTGGCAGGGCTGGC
CACAAGGGGCGTCCACCCAGGACACCGTCTGGGTCCCACTCAGGCCCAGTCCCCTCCCAG
GGCCTGGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE5626 Download

>KIEE5626  483 aa
MAGFAELGLSSWLVEQCRQLGLKQPTPVQLGCIPAILEGRDCLGCAKTGSGKTAAFVLPI
LQKLSEDPYGIFCLVLTPTRELAYQIAEQFRVLGKPLGLKDCIIVGGMDMVAQALELSRK
PHVVIATPGRLADHLRSSNTFSIKKIRFLVMDEADRLLEQGCTDFTVDLEAILAAVPARR
QTLLFSATLTDTLRELQGLATNQPFFWEAQAPVSTVEQLDQRYLLVPEKVKDAYLVHLIQ
RFQDEHEDWSIIIFTNTCKTCQILCMMLRKFSFPTVALHSMMKQKERFAALAKFKSSIYR
ILIATDVASRGLDIPTVQVVINHNTPGLPKIYIHRVGRTARAGRQGQAITLVTQYDIHLV
HAIEEQIKKKLEEFSVEEAEVLQILTQVNVVRRECEIKLEAAHFDEKKEINKRKQLILEG
KDPDLEAKRKAELAKIKQKNRRFKEKVEETLKRQKAGRAGHKGRPPRTPSGSHSGPVPSQ
GLV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5626 Download

>pFN21AE5626  6154 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGCTTCGCGGAGCTCGGGCTGTCATCGTG
GCTCGTGGAACAATGTCGGCAGCTGGGTTTGAAGCAGCCCACGCCCGTGCAGCTCGGCTG
CATCCCCGCCATCCTGGAGGGTCGAGACTGCTTGGGCTGTGCTAAGACAGGCAGTGGGAA
GACAGCAGCGTTTGTCCTTCCCATCTTGCAGAAGCTGTCTGAGGATCCCTATGGCATCTT
CTGCCTCGTCCTGACACCCACCAGGGAGCTGGCCTACCAGATCGCAGAGCAGTTCCGGGT
CCTGGGGAAGCCTCTAGGGCTGAAAGACTGCATCATCGTCGGTGGCATGGACATGGTGGC
CCAGGCGCTGGAGCTCTCTCGGAAACCACACGTGGTCATCGCCACGCCGGGGCGCCTGGC
AGATCACCTGCGCAGCTCCAACACTTTTAGTATAAAGAAGATCCGCTTCCTGGTGATGGA
TGAGGCAGACCGGCTGCTGGAACAGGGCTGCACTGACTTCACCGTGGACCTGGAGGCCAT
CCTGGCGGCTGTGCCGGCCCGCAGGCAGACACTGCTGTTCAGCGCCACGCTGACCGACAC
ACTCCGGGAGCTGCAGGGTCTGGCCACCAACCAGCCCTTCTTCTGGGAAGCACAGGCCCC
GGTGAGCACCGTGGAGCAGCTGGACCAGCGCTACCTGCTGGTGCCTGAGAAGGTCAAGGA
CGCCTACCTGGTCCACCTGATCCAGCGCTTCCAGGATGAGCACGAGGACTGGTCCATTAT
CATCTTCACCAACACGTGCAAGACCTGCCAGATTCTGTGCATGATGCTGCGCAAATTCAG
CTTCCCCACCGTGGCTCTGCACTCCATGATGAAGCAGAAAGAACGCTTTGCCGCCCTAGC
CAAGTTCAAGTCCAGCATCTACCGGATCCTGATCGCAACAGACGTGGCCTCCCGGGGCCT
GGACATCCCTACGGTACAGGTGGTCATCAACCACAACACCCCCGGGCTCCCCAAGATCTA
CATCCACCGAGTCGGCCGGACGGCCCGTGCAGGGCGGCAGGGTCAGGCCATCACGCTGGT
GACACAGTACGACATCCACCTGGTGCACGCCATCGAGGAGCAGATCAAGAAGAAGCTGGA
GGAGTTCTCCGTGGAAGAGGCCGAGGTGCTACAGATCCTCACACAGGTCAACGTGGTGCG
AAGAGAGTGTGAGATCAAACTGGAGGCGGCCCACTTTGACGAAAAGAAGGAGATCAACAA
ACGGAAGCAGCTGATCCTGGAGGGGAAGGACCCTGACCTGGAGGCCAAGCGCAAGGCTGA
GCTGGCCAAGATCAAGCAGAAGAACCGGCGCTTCAAGGAGAAGGTGGAGGAGACGCTGAA
GCGACAGAAGGCTGGCAGGGCTGGCCACAAGGGGCGTCCACCCAGGACACCGTCTGGGTC
CCACTCAGGCCCAGTCCCCTCCCAGGGCCTGGTCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT