################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbvCI BciVI BclI BfrI BlnI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI KpnI KspI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 12 CGCG CG^CG AcsI 808 AAATTC [GA]^AATT[CT] Alw44I 1078 GTGCAC G^TGCAC ApaLI 1078 GTGCAC G^TGCAC ApoI 808 AAATTC [GA]^AATT[CT] AspI 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 386 GGCGCC GGCGC^C BbsI 153 GAAGAC GAAGAC(2/6) BcgI 305 GCATCATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 989 AGATCT A^GATCT BlpI 1291 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1291 GCTGAGC GC^T[ACGT]AGC BpuAI 153 GAAGAC GAAGAC(2/6) BsaWI 896 ACCGGA [AT]^CCGG[AT] BsiEI 1008 CGGCCG CG[GA][CT]^CG BsmBI 1345 GAGACG CGTCTC(1/5) BstUI 12 CGCG CG^CG CelII 1291 GCTGAGC GC^T[ACGT]AGC DraIII 1072 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1008 CGGCCG C^GGCCG EclXI 1008 CGGCCG C^GGCCG Eco52I 1008 CGGCCG C^GGCCG EcoNI 1152 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 1162 GTCAAC GT[CT]^[GA]AC HindII 1162 GTCAAC GT[CT]^[GA]AC KasI 386 GGCGCC G^GCGCC MaeIII 1054 GTGAC ^GT[ACGT]AC MvnI 12 CGCG CG^CG NaeI 528 GCCGGC GCC^GGC NarI 386 GGCGCC GG^CGCC NgoMIV 528 GCCGGC G^CCGGC PflFI 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1004 GAGTC GAGTC(4/5) PstI 586 CTGCAG CTGCA^G SfoI 386 GGCGCC GGC^GCC TfiI 786 GATTC G^A[AT]TC Tsp45I 1054 GTGAC ^GT[GC]AC Tsp509I 809 AATT ^AATT Tth111I 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 233 CCACCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AfaI 949 GTAC GT^AC 1062 GTAC AflIII 363 ACACGT A^C[GA][CT]GT 767 ACACGT Aor51HI 662 AGCGCT AGC^GCT 719 AGCGCT AviII 404 TGCGCA TGC^GCA 803 TGCGCA BamHI 198 GGATCC G^GATCC 899 GGATCC BanI 386 GGCGCC G^G[CT][GA]CC 675 GGTGCC BbrPI 364 CACGTG CAC^GTG 768 CACGTG BfaI 289 CTAG C^TAG 871 CTAG BglI 285 GCCTCTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 511 GCCATCCTGGC BsaAI 364 CACGTG [CT]AC^GT[GA] 768 CACGTG BsaBI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 990 GATCTACATC BsmAI 119 GAGAC GTCTC(1/5) 1345 GAGAC BsmFI 1413 GTCCC GGGAC(10/14) 1429 GTCCC BssSI 736 CACGAG CACGAG(-5/-1) 37 CTCGTG BstXI 236 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1043 CCATCACGCTGG EarI 1129 GAAGAG CTCTTC(1/4) 1175 GAAGAG Eco47III 662 AGCGCT AGC^GCT 719 AGCGCT FauI 100 CCCGC CCCGC(4/6) 533 CCCGC FspI 404 TGCGCA TGC^GCA 803 TGCGCA HinfI 786 GATTC G^A[ACGT]TC 1004 GAGTC Ksp632I 1129 GAAGAG CTCTTC(1/4) 1175 GAAGAG MaeI 289 CTAG C^TAG 871 CTAG MamI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 990 GATCTACATC PflMI 336 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1437 CCAGGGCCTGG PmaCI 364 CACGTG CAC^GTG 768 CACGTG PmlI 364 CACGTG CAC^GTG 768 CACGTG RsaI 949 GTAC GT^AC 1062 GTAC SacI 16 GAGCTC GAGCT^C 346 GAGCTC SexAI 701 ACCTGGT A^CC[AT]GGT 1073 ACCTGGT SfcI 586 CTGCAG C^T[GA][CT]AG 1144 CTACAG SmaI 926 CCCGGG CCC^GGG 976 CCCGGG TaqI 117 TCGA T^CGA 1088 TCGA TthHB8I 117 TCGA T^CGA 1088 TCGA Van91I 336 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1437 CCAGGGCCTGG XmaCI 926 CCCGGG C^CCGGG 976 CCCGGG XmaI 926 CCCGGG C^CCGGG 976 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcyI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI TseI TspRI XhoII