(Circular) (Six-base) MAPSORT of: pFN21AE5626.seq Check: 4264 from: 1 to: 6154 pFN21AE5626 6154 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2415 2415 Size: 6154 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3377 4217 5601 5869 1632 Size: 1745 840 1384 268 1917 Fragments arranged by size: 1917 1745 1384 840 268 AfeI AGC'GCT Cuts at: 2669 2726 2669 Size: 57 6097 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 2368 2772 5053 6030 1203 Size: 1165 404 2281 977 1327 Fragments arranged by size: 2281 1327 1165 977 404 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 2084 3056 1166 Size: 918 972 4264 Fragments arranged by size: 4264 972 918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2822 1184 Size: 1638 4516 AlwNI CAG_nnn'CTG Cuts at: 2194 2347 2551 2599 2794 3448 3897 5469 2194 Size: 153 204 48 195 654 449 1572 2879 Fragments arranged by size: 2879 1572 654 449 204 195 153 48 ApaLI G'TGCA_C Cuts at: 3083 4211 5367 3083 Size: 1128 1156 3870 Fragments arranged by size: 3870 1156 1128 ApoI r'AATT_y Cuts at: 1493 2813 3463 3689 3841 5950 1493 Size: 1320 650 226 152 2109 1697 Fragments arranged by size: 2109 1697 1320 650 226 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2931 2981 3479 1870 Size: 73 81 907 50 498 4545 Fragments arranged by size: 4545 907 498 81 73 50 BamHI G'GATC_C Cuts at: 2203 2904 3484 3851 2203 Size: 701 580 367 4506 Fragments arranged by size: 4506 701 580 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 2680 3475 4930 618 Size: 325 217 627 604 289 795 1455 1842 Fragments arranged by size: 1842 1455 795 627 604 325 289 217 BanII G_rGCy'C Cuts at: 1391 2025 2355 2988 3473 1391 Size: 634 330 633 485 4072 Fragments arranged by size: 4072 634 633 485 330 BbeI G_GCGC'C Cuts at: 2395 2395 Size: 6154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 928 Size: 971 266 4917 Fragments arranged by size: 4917 971 266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2299 2333 4345 4379 1903 Size: 34 362 34 2012 34 3678 Fragments arranged by size: 3678 2012 362 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2296 2522 4765 Size: 107 122 71 631 605 624 226 2243 Cuts at: 4765 136 Size: 1525 Fragments arranged by size: 2243 1525 631 624 605 226 122 107 71 BglII A'GATC_T Cuts at: 2994 6149 2994 Size: 3155 2999 BlpI GC'TnA_GC Cuts at: 3297 3571 3297 Size: 274 5880 Bme1580I G_kGCm'C Cuts at: 1790 3087 4215 5371 1790 Size: 1297 1128 1156 2573 Fragments arranged by size: 2573 1297 1156 1128 BmgBI CAC'GTC Cuts at: 1318 2923 1318 Size: 1605 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3425 3885 4843 Size: 416 53 83 399 24 2008 460 958 Cuts at: 4843 442 Size: 1753 Fragments arranged by size: 2008 1753 958 460 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3142 3142 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2369 2531 3137 3221 3278 3299 4814 Size: 896 234 162 606 84 57 21 1515 Cuts at: 4814 1239 Size: 2579 Fragments arranged by size: 2579 1515 896 606 234 162 84 57 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4817 882 Size: 617 3318 2219 Fragments arranged by size: 3318 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 2775 3945 493 Size: 711 1167 404 1170 2702 Fragments arranged by size: 2702 1170 1167 711 404 BsaBI GATnn'nnATC Cuts at: 2909 2999 6148 2909 Size: 90 3149 2915 Fragments arranged by size: 3149 2915 90 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2392 2700 3394 3961 Size: 53 83 186 936 859 308 694 567 Cuts at: 3961 4343 4978 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 859 694 635 567 382 308 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 4587 4985 5259 5406 5817 2901 Size: 1686 398 274 147 411 3238 Fragments arranged by size: 3238 1686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2912 2942 1178 Size: 30 165 30 1509 30 4390 Fragments arranged by size: 4390 1509 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3110 3134 3362 1186 Size: 1924 24 228 3978 Fragments arranged by size: 3978 1924 228 24 BseYI C'CCAG_C Cuts at: 2062 5357 5853 2062 Size: 3295 496 2363 Fragments arranged by size: 3295 2363 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2473 2822 3048 1163 Size: 573 372 365 349 226 4269 Fragments arranged by size: 4269 573 372 365 349 226 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3016 3604 4365 5393 1540 Size: 275 186 1015 588 761 1028 2301 Fragments arranged by size: 2301 1028 1015 761 588 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2355 2648 2742 3087 4215 4300 5371 2025 Size: 330 293 94 345 1128 85 1071 2808 Fragments arranged by size: 2808 1128 1071 345 330 293 94 85 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 BsmBI CGTCTCn'nnnn_ Cuts at: 3344 3344 Size: 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2355 2648 2742 2988 3087 3473 Size: 399 235 330 293 94 246 99 386 Cuts at: 3473 4215 4300 5371 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 386 330 293 246 235 99 94 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 4043 5773 4043 Size: 1730 4424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 2682 2792 3509 844 Size: 1571 267 110 717 3489 Fragments arranged by size: 3489 1571 717 267 110 BsrBI CCG'CTC Cuts at: 1394 1823 4041 1394 Size: 429 2218 3507 Fragments arranged by size: 3507 2218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4649 4823 65 Size: 1183 3401 174 1396 Fragments arranged by size: 3401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2469 2533 3324 4798 4985 1700 Size: 110 659 64 791 1474 187 2869 Fragments arranged by size: 2869 1474 791 659 187 110 64 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 2042 2741 4214 5226 2042 Size: 699 1473 1012 2970 Fragments arranged by size: 2970 1473 1012 699 BstAPI GCAn_nnn'nTGC Cuts at: 794 2220 2551 2784 794 Size: 1426 331 233 4164 Fragments arranged by size: 4164 1426 331 233 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstXI CCAn_nnnn'nTGG Cuts at: 2248 3055 2248 Size: 807 5347 BstYI r'GATC_y Cuts at: 1972 2203 2439 2904 2994 3153 3484 3851 4236 Size: 231 236 465 90 159 331 367 385 Cuts at: 4236 4253 5705 5791 5803 6149 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 465 385 367 346 331 236 231 159 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2502 2649 2829 3129 513 Size: 552 939 498 147 180 300 3538 Fragments arranged by size: 3538 939 552 498 300 180 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 487 Size: 683 56 1136 4279 Fragments arranged by size: 4279 1136 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2549 3670 4464 4484 2158 Size: 391 1121 794 20 3828 Fragments arranged by size: 3828 1121 794 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2904 2939 529 Size: 35 2340 35 3744 Fragments arranged by size: 3744 2340 35 35 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DraIII CAC_nnn'GTG Cuts at: 3082 3082 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2601 3013 3303 Size: 54 1425 50 275 18 771 412 290 Cuts at: 3303 3382 3601 4490 6119 8 Size: 79 219 889 1629 43 Fragments arranged by size: 1629 1425 889 771 412 290 275 219 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 3601 1537 Size: 275 1201 588 4090 Fragments arranged by size: 4090 1201 588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3129 3175 4084 1353 Size: 1776 46 909 3423 Fragments arranged by size: 3423 1776 909 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2353 2023 Size: 330 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2369 2531 3137 3221 3278 3299 Size: 393 503 234 162 606 84 57 21 Cuts at: 3299 3377 4217 4814 5601 5869 1239 Size: 78 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 606 597 503 393 268 234 162 84 78 57 21 EcoNI CCTnn'n_nnAGG Cuts at: 3161 3161 Size: 6154 EcoO109I rG'GnC_Cy Cuts at: 2278 2635 2935 3268 3417 3445 2278 Size: 357 300 333 149 28 4987 Fragments arranged by size: 4987 357 333 300 149 28 EcoRI G'AATT_C Cuts at: 3463 3841 3463 Size: 378 5776 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 6154 FspI TGC'GCA Cuts at: 2411 2810 4660 2411 Size: 399 1850 3905 Fragments arranged by size: 3905 1850 399 HaeII r_GCGC'y Cuts at: 1678 2348 2395 2565 2671 2728 3331 5301 1678 Size: 670 47 170 106 57 603 1970 2531 Fragments arranged by size: 2531 1970 670 603 170 106 57 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 2912 2944 4795 Size: 32 135 32 1163 32 145 32 1851 Cuts at: 4795 4827 4869 4901 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1851 1163 145 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3169 3498 3744 669 Size: 988 281 1231 329 246 3079 Fragments arranged by size: 3079 1231 988 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KasI G'GCGC_C Cuts at: 2391 2391 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 5268 5452 1305 Size: 1137 2826 184 2007 Fragments arranged by size: 2826 2007 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 3305 3384 10 Size: 54 1425 343 771 702 79 2780 Fragments arranged by size: 2780 1425 771 702 343 79 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2330 3056 4112 4471 4630 518 Size: 648 918 246 726 1056 359 159 2042 Fragments arranged by size: 2042 1056 918 726 648 359 246 159 MspA1I CmG'CkG Cuts at: 1606 2062 2659 3250 3570 4247 5395 5640 1606 Size: 456 597 591 320 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 597 591 456 320 245 NaeI GCC'GGC Cuts at: 2535 2535 Size: 6154 NarI GG'CG_CC Cuts at: 2392 2392 Size: 6154 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4663 Fragments arranged by size: 4663 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NgoMIV G'CCGG_C Cuts at: 2533 2533 Size: 6154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 3512 5057 1132 Size: 97 2283 1545 2229 Fragments arranged by size: 2283 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3442 1348 Size: 2094 4060 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 3448 1108 Size: 1239 1101 3814 Fragments arranged by size: 3814 1239 1101 PfoI T'CCnGG_A Cuts at: 2112 2583 2730 3255 2112 Size: 471 147 525 5011 Fragments arranged by size: 5011 525 471 147 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PmlI CAC'GTG Cuts at: 2371 2775 2371 Size: 404 5750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 3268 3417 2278 Size: 990 149 5015 Fragments arranged by size: 5015 990 149 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PstI C_TGCA'G Cuts at: 830 2595 3506 830 Size: 1765 911 3478 Fragments arranged by size: 3478 1765 911 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2062 2659 3250 2062 Size: 597 591 4966 Fragments arranged by size: 4966 597 591 SacI G_AGCT'C Cuts at: 2025 2355 2025 Size: 330 5824 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SanDI GG'GwC_CC Cuts at: 3417 3417 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SexAI A'CCwGG_T Cuts at: 2706 3078 2706 Size: 372 5782 SfcI C'TryA_G Cuts at: 826 1045 2591 3149 3502 4637 5318 5509 826 Size: 219 1546 558 353 1135 681 191 1471 Fragments arranged by size: 1546 1471 1135 681 558 353 219 191 SfoI GGC'GCC Cuts at: 2393 2393 Size: 6154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 2933 2983 3481 1872 Size: 1061 50 498 4545 Fragments arranged by size: 4545 1061 498 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 4258 5159 5421 820 Size: 197 926 2315 901 262 1553 Fragments arranged by size: 2315 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 4078 5 Size: 47 4026 2081 Fragments arranged by size: 4026 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 2004 3593 513 Size: 552 939 1589 3074 Fragments arranged by size: 3074 1589 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 2588 3415 4181 4198 4351 4536 Size: 1255 671 337 827 766 17 153 185 Cuts at: 4536 4981 325 Size: 445 1498 Fragments arranged by size: 1498 1255 827 766 671 445 337 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4400 6112 96 Size: 274 80 33 51 1443 2423 1712 138 Fragments arranged by size: 2423 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3010 3042 6114 6146 3010 Size: 32 3072 32 3018 Fragments arranged by size: 3072 3018 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3409 1316 Size: 2093 4061 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2245 2245 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 2931 2981 3479 1870 Size: 1061 50 498 4545 Fragments arranged by size: 4545 1061 498 50 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI