(Linear) (Six-base) MAPSORT of: KIEE5626.seq Check: 5164 from: 1 to: 1449 KIEE5626.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 410 1449 Size: 410 1039 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1372 1449 Size: 1372 77 AfeI AGC'GCT Cuts at: 0 664 721 1449 Size: 664 57 728 Fragments arranged by size: 728 664 57 AflIII A'CryG_T Cuts at: 0 363 767 1449 Size: 363 404 682 Fragments arranged by size: 682 404 363 AleI CACnn'nnGTG Cuts at: 0 79 1051 1449 Size: 79 972 398 Fragments arranged by size: 972 398 79 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 817 1449 Size: 817 632 AlwNI CAG_nnn'CTG Cuts at: 0 189 342 546 594 789 1443 1449 Size: 189 153 204 48 195 654 6 Fragments arranged by size: 654 204 195 189 153 48 6 ApaLI G'TGCA_C Cuts at: 0 1078 1449 Size: 1078 371 ApoI r'AATT_y Cuts at: 0 808 1449 Size: 808 641 AvaI C'yCGr_G Cuts at: 0 19 926 976 1449 Size: 19 907 50 473 Fragments arranged by size: 907 473 50 19 BamHI G'GATC_C Cuts at: 0 198 899 1449 Size: 198 701 550 Fragments arranged by size: 701 550 198 BanI G'GyrC_C Cuts at: 0 386 675 1449 Size: 386 289 774 Fragments arranged by size: 774 386 289 BanII G_rGCy'C Cuts at: 0 20 350 983 1449 Size: 20 330 633 466 Fragments arranged by size: 633 466 330 20 BbeI G_GCGC'C Cuts at: 0 390 1449 Size: 390 1059 BbsI GAAGACnn'nnnn_ Cuts at: 0 160 1449 Size: 160 1289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 294 328 1449 Size: 294 34 1121 Fragments arranged by size: 1121 294 34 BglI GCCn_nnn'nGGC Cuts at: 0 291 517 1449 Size: 291 226 932 Fragments arranged by size: 932 291 226 BglII A'GATC_T Cuts at: 0 989 1449 Size: 989 460 BlpI GC'TnA_GC Cuts at: 0 1292 1449 Size: 1292 157 Bme1580I G_kGCm'C Cuts at: 0 1082 1449 Size: 1082 367 BmgBI CAC'GTC Cuts at: 0 918 1449 Size: 918 531 BmrI ACTGGGnnnn_n' Cuts at: 0 1420 1449 Size: 1420 29 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1137 1449 Size: 1137 312 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 364 526 1132 1216 1273 1294 1449 Size: 130 234 162 606 84 57 21 155 Fragments arranged by size: 606 234 162 155 130 84 57 21 BsaAI yAC'GTr Cuts at: 0 366 770 1449 Size: 366 404 679 Fragments arranged by size: 679 404 366 BsaBI GATnn'nnATC Cuts at: 0 904 994 1449 Size: 904 90 455 Fragments arranged by size: 904 455 90 BsaHI Gr'CG_yC Cuts at: 0 387 695 1389 1449 Size: 387 308 694 60 Fragments arranged by size: 694 387 308 60 BsaWI w'CCGG_w Cuts at: 0 896 1449 Size: 896 553 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 907 937 1449 Size: 907 30 512 Fragments arranged by size: 907 512 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1105 1129 1357 1449 Size: 1105 24 228 92 Fragments arranged by size: 1105 228 92 24 BseYI C'CCAG_C Cuts at: 0 57 1449 Size: 57 1392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 468 817 1043 1449 Size: 103 365 349 226 406 Fragments arranged by size: 406 365 349 226 103 BsiEI CG_ry'CG Cuts at: 0 1011 1449 Size: 1011 438 BsiHKAI G_wGCw'C Cuts at: 0 20 350 643 737 1082 1449 Size: 20 330 293 94 345 367 Fragments arranged by size: 367 345 330 293 94 20 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1339 1449 Size: 1339 110 Bsp1286I G_dGCh'C Cuts at: 0 20 350 643 737 983 1082 1449 Size: 20 330 293 94 246 99 367 Fragments arranged by size: 367 330 293 246 99 94 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 410 677 787 1449 Size: 410 267 110 662 Fragments arranged by size: 662 410 267 110 BsrFI r'CCGG_y Cuts at: 0 464 528 1319 1449 Size: 464 64 791 130 Fragments arranged by size: 791 464 130 64 BssSI C'ACGA_G Cuts at: 0 37 736 1449 Size: 37 699 713 Fragments arranged by size: 713 699 37 BstAPI GCAn_nnn'nTGC Cuts at: 0 215 546 779 1449 Size: 215 331 233 670 Fragments arranged by size: 670 331 233 215 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 1050 1449 Size: 243 807 399 Fragments arranged by size: 807 399 243 BstYI r'GATC_y Cuts at: 0 198 434 899 989 1148 1449 Size: 198 236 465 90 159 301 Fragments arranged by size: 465 301 236 198 159 90 BtgI C'CryG_G Cuts at: 0 497 644 824 1124 1449 Size: 497 147 180 300 325 Fragments arranged by size: 497 325 300 180 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 357 1449 Size: 357 1092 BtsI GCAGTG_nn' Cuts at: 0 153 544 1449 Size: 153 391 905 Fragments arranged by size: 905 391 153 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 899 934 1449 Size: 899 35 515 Fragments arranged by size: 899 515 35 DraIII CAC_nnn'GTG Cuts at: 0 1077 1449 Size: 1077 372 EaeI y'GGCC_r Cuts at: 0 596 1008 1298 1377 1449 Size: 596 412 290 79 72 Fragments arranged by size: 596 412 290 79 72 EagI C'GGCC_G Cuts at: 0 1008 1449 Size: 1008 441 EarI CTCTTCn'nnn_ Cuts at: 0 1124 1170 1449 Size: 1124 46 279 Fragments arranged by size: 1124 279 46 EcoICRI GAG'CTC Cuts at: 0 18 348 1449 Size: 18 330 1101 Fragments arranged by size: 1101 330 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 364 526 1132 1216 1273 1294 1372 Size: 130 234 162 606 84 57 21 78 Cuts at: 1372 1449 Size: 77 Fragments arranged by size: 606 234 162 130 84 78 77 57 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1156 1449 Size: 1156 293 EcoO109I rG'GnC_Cy Cuts at: 0 273 630 930 1263 1412 1440 1449 Size: 273 357 300 333 149 28 9 Fragments arranged by size: 357 333 300 273 149 28 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 456 1449 Size: 456 993 FspI TGC'GCA Cuts at: 0 406 805 1449 Size: 406 399 644 Fragments arranged by size: 644 406 399 HaeII r_GCGC'y Cuts at: 0 343 390 560 666 723 1326 1449 Size: 343 47 170 106 57 603 123 Fragments arranged by size: 603 343 170 123 106 57 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 907 939 1449 Size: 730 32 145 32 510 Fragments arranged by size: 730 510 145 32 32 HincII GTy'rAC Cuts at: 0 1164 1449 Size: 1164 285 KasI G'GCGC_C Cuts at: 0 386 1449 Size: 386 1063 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 437 1449 Size: 437 1012 MscI TGG'CCA Cuts at: 0 598 1300 1379 1449 Size: 598 702 79 70 Fragments arranged by size: 702 598 79 70 MslI CAynn'nnrTG Cuts at: 0 79 325 1051 1449 Size: 79 246 726 398 Fragments arranged by size: 726 398 246 79 MspA1I CmG'CkG Cuts at: 0 57 654 1245 1449 Size: 57 597 591 204 Fragments arranged by size: 597 591 204 57 NaeI GCC'GGC Cuts at: 0 530 1449 Size: 530 919 NarI GG'CG_CC Cuts at: 0 387 1449 Size: 387 1062 NgoMIV G'CCGG_C Cuts at: 0 528 1449 Size: 528 921 PasI CC'CwG_GG Cuts at: 0 1437 1449 Size: 1437 12 PflMI CCAn_nnn'nTGG Cuts at: 0 342 1443 1449 Size: 342 1101 6 Fragments arranged by size: 1101 342 6 PfoI T'CCnGG_A Cuts at: 0 107 578 725 1250 1449 Size: 107 471 147 525 199 Fragments arranged by size: 525 471 199 147 107 PmlI CAC'GTG Cuts at: 0 366 770 1449 Size: 366 404 679 Fragments arranged by size: 679 404 366 PpuMI rG'GwC_Cy Cuts at: 0 273 1263 1412 1449 Size: 273 990 149 37 Fragments arranged by size: 990 273 149 37 PstI C_TGCA'G Cuts at: 0 590 1449 Size: 590 859 PvuII CAG'CTG Cuts at: 0 57 654 1245 1449 Size: 57 597 591 204 Fragments arranged by size: 597 591 204 57 SacI G_AGCT'C Cuts at: 0 20 350 1449 Size: 20 330 1099 Fragments arranged by size: 1099 330 20 SanDI GG'GwC_CC Cuts at: 0 1412 1449 Size: 1412 37 SexAI A'CCwGG_T Cuts at: 0 701 1073 1449 Size: 701 372 376 Fragments arranged by size: 701 376 372 SfcI C'TryA_G Cuts at: 0 586 1144 1449 Size: 586 558 305 Fragments arranged by size: 586 558 305 SfoI GGC'GCC Cuts at: 0 388 1449 Size: 388 1061 SmaI CCC'GGG Cuts at: 0 928 978 1449 Size: 928 50 471 Fragments arranged by size: 928 471 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 246 583 1410 1449 Size: 246 337 827 39 Fragments arranged by size: 827 337 246 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1005 1037 1449 Size: 1005 32 412 Fragments arranged by size: 1005 412 32 Tth111I GACn'n_nGTC Cuts at: 0 1404 1449 Size: 1404 45 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 240 1449 Size: 240 1209 XmaI C'CCGG_G Cuts at: 0 926 976 1449 Size: 926 50 473 Fragments arranged by size: 926 473 50 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PmlI PpuMI PstI PvuII SacI SanDI SexAI SfcI SfoI SmaI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BmtI Bpu10I BpuEI BsaI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EciI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI XbaI XhoI XmnI ZraI