RPF1 ORF clone pFN21AE5414

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5414

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Clone info.

Species

human

Clone Name

pFN21AE5414 Sequence (with vector) Restriction Map

Product ID

FHC12161

ORF Sequence

DNA sequence (1047 bp)
Protein sequence (349 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5414

Symbol

RPF1

Alias : BXDC5

Description

ribosome production factor 1 homolog

Original Clone ID

oc95414

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1047 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 349 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS695.1 2.3e-125 99.7 RPF1 CCDS2160.1 1.6e-20 30.4 IMP4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007109 146 318 PF04427 Brix domain HMMSmart IPR007109 145 319 SM00879 Brix domain ProfileScan IPR007109 142 325 PS50833 Brix domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001307233 289 612981 30.2 83.1 NP_219484 291 612981 30.4 83.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5414 Download

>KIEE5414  1047 bp
ATGGCGAAAGCCGGGGATAAGAGCGGCAGCAGCGGGAAGAAAAGTCTAAAACGGAAAGCC
GCTGCCGAAGAACTTCAGGAGGCTGCAGGCGCTGGGGATGGGGCGACGGAAAACGGGGTC
CAACCCCCGAAAGCGGCTGCCTTTCCGCCAGGCTTTAGCATTTCGGAGATTAAAAACAAA
CAGCGGCGACACTTAATGTTCACGCGGTGGAAACAGCAGCAGCGGAAGGAAAAGTTGGCA
GCTAAGAAAAAACTTAAAAAAGAAAGAGAGGCTCTTGGCGATAAGGCTCCACCAAAGCCT
GTACCCAAGACCATTGACAACCAGCGAGTGTATGATGAAACCACAGTAGACCCTAATGAT
GAAGAGGTCGCTTATGATGAAGCTACAGATGAATTTGCTTCTTACTTCAACAAACAGACT
TCTCCCAAGATTCTCATCACAACATCAGATAGACCTCATGGGAGAACAGTACGACTCTGT
GAACAGCTCTCCACAGTTATACCAAACTCACATGTTTATTACAGAAGAGGACTGGCTCTG
AAAAAAATTATTCCACAGTGCATCGCAAGAGATTTCACAGACCTGATTGTTATTAATGAA
GATCGTAAAACCCCAAATGGACTTATTTTGAGTCACTTGCCAAATGGCCCAACTGCTCAT
TTTAAAATGAGCAGTGTTCGTCTTCGTAAAGAAATTAAGAGAAGAGGCAAGGACCCCACA
GAACACATACCTGAAATAATTCTGAATAATTTTACAACACGGCTGGGTCATTCAATTGGA
CGTATGTTTGCATCTCTCTTTCCTCATAATCCTCAATTTATCGGAAGGCAGGTTGCCACA
TTCCACAATCAACGGGATTACATATTCTTCAGATTTCACAGATACATATTCAGGAGTGAA
AAGAAAGTGGGAATTCAGGAACTTGGACCACGTTTTACCTTAAAATTAAGGTCTCTTCAG
AAAGGAACCTTTGATTCTAAATATGGAGAGTATGAATGGGTCCATAAGCCCCGGGAAATG
GATACAAGTAGAAGAAAATTCCATTTA

ORF Protein

Cloned ORF protein sequence for pFN21AE5414 Download

>KIEE5414  349 aa
MAKAGDKSGSSGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNK
QRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTIDNQRVYDETTVDPND
EEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQLSTVIPNSHVYYRRGLAL
KKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPT
EHIPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSE
KKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5414 Download

>pFN21AE5414  5752 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGAAAGCCGGGGATAAGAGCGGCAGCAGCGG
GAAGAAAAGTCTAAAACGGAAAGCCGCTGCCGAAGAACTTCAGGAGGCTGCAGGCGCTGG
GGATGGGGCGACGGAAAACGGGGTCCAACCCCCGAAAGCGGCTGCCTTTCCGCCAGGCTT
TAGCATTTCGGAGATTAAAAACAAACAGCGGCGACACTTAATGTTCACGCGGTGGAAACA
GCAGCAGCGGAAGGAAAAGTTGGCAGCTAAGAAAAAACTTAAAAAAGAAAGAGAGGCTCT
TGGCGATAAGGCTCCACCAAAGCCTGTACCCAAGACCATTGACAACCAGCGAGTGTATGA
TGAAACCACAGTAGACCCTAATGATGAAGAGGTCGCTTATGATGAAGCTACAGATGAATT
TGCTTCTTACTTCAACAAACAGACTTCTCCCAAGATTCTCATCACAACATCAGATAGACC
TCATGGGAGAACAGTACGACTCTGTGAACAGCTCTCCACAGTTATACCAAACTCACATGT
TTATTACAGAAGAGGACTGGCTCTGAAAAAAATTATTCCACAGTGCATCGCAAGAGATTT
CACAGACCTGATTGTTATTAATGAAGATCGTAAAACCCCAAATGGACTTATTTTGAGTCA
CTTGCCAAATGGCCCAACTGCTCATTTTAAAATGAGCAGTGTTCGTCTTCGTAAAGAAAT
TAAGAGAAGAGGCAAGGACCCCACAGAACACATACCTGAAATAATTCTGAATAATTTTAC
AACACGGCTGGGTCATTCAATTGGACGTATGTTTGCATCTCTCTTTCCTCATAATCCTCA
ATTTATCGGAAGGCAGGTTGCCACATTCCACAATCAACGGGATTACATATTCTTCAGATT
TCACAGATACATATTCAGGAGTGAAAAGAAAGTGGGAATTCAGGAACTTGGACCACGTTT
TACCTTAAAATTAAGGTCTCTTCAGAAAGGAACCTTTGATTCTAAATATGGAGAGTATGA
ATGGGTCCATAAGCCCCGGGAAATGGATACAAGTAGAAGAAAATTCCATTTAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT