Restriction map for
pFN21AE5414
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeI MflI MluI MluNI
MroI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 346 GTAGAC GT^[AC][GT]AC
AccII 203 CGCG CG^CG
AflIII 510 ACATGT A^C[GA][CT]GT
AlwNI 86 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 592 ATTAAT AT^TAAT
AsnI 592 ATTAAT AT^TAAT
AvaI 1010 CCCGGG C^[CT]CG[GA]G
BbsI 680 GTCTTC GAAGAC(2/6)
BciVI 1020 GGATAC GTATCC(6/5)
BpuAI 680 GTCTTC GAAGAC(2/6)
BsaBI 429 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 950 GGTCTC GGTCTC(1/5)
BsmAI 951 GTCTC GTCTC(1/5)
BsoBI 1010 CCCGGG C^[CT]CG[GA]G
BspLU11I 510 ACATGT A^CATGT
BspMI 828 GCAGGT ACCTGC(4/8)
BsrBI 21 GAGCGG CCGCTC(-3/-3)
BsrI 531 ACTGG ACTGG(1/-1)
BstF5I 96 GGATG GGATG(2/0)
BstNI 148 CCAGG CC^[AT]GG
BstUI 203 CGCG CG^CG
Cac8I 85 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 89 GCGC GCG^C
DdeI 242 CTAAG C^T[ACGT]AG
DpnII 601 GATC ^GATC
DraI 661 TTTAAA TTT^AAA
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 201 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 911 GAATTC G^AATTC
EcoRII 148 CCAGG ^CC[AT]GG
FauI 32 GCGGG CCCGC(4/6)
FokI 96 GGATG GGATG(9/13)
HaeII 88 GGCGCT [GA]GCGC^[CT]
HaeIII 646 GGCC GG^CC
HhaI 89 GCGC GCG^C
HinP1I 89 GCGC G^CGC
MaeIII 632 GTCAC ^GT[ACGT]AC
MamI 429 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 601 GATC ^GATC
MfeI 773 CAATTG C^AATTG
MunI 773 CAATTG C^AATTG
MvaI 148 CCAGG CC^[AT]GG
MvnI 203 CGCG CG^CG
NdeII 601 GATC ^GATC
NspI 510 ACATGT [GA]CATG^[CT]
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 592 ATTAAT AT^TAAT
PstI 83 CTGCAG CTGCA^G
Sau3AI 601 GATC ^GATC
SmaI 1010 CCCGGG CCC^GGG
Tsp45I 632 GTCAC ^GT[GC]AC
XmaCI 1010 CCCGGG C^CCGGG
XmaI 1010 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 301 GTAC GT^AC
469 GTAC
Asp700I 67 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAAATAATTC
BsiYI 126 CCCGAAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1011 CCGGGAAATGG
BslI 126 CCCGAAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1011 CCGGGAAATGG
HapII 11 CCGG C^CGG
1011 CCGG
HpaII 11 CCGG C^CGG
1011 CCGG
MaeII 780 ACGT A^CGT
930 ACGT
MspI 11 CCGG C^CGG
1011 CCGG
MwoI 23 GCGGCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
82 GCTGCAGGCGC
NlaIII 457 CATG CATG^
511 CATG
PleI 473 GACTC GAGTC(4/5)
630 GAGTC
RsaI 301 GTAC GT^AC
469 GTAC
SfaNI 560 GCATC GCATC(5/9)
790 GCATC
SfcI 83 CTGCAG C^T[GA][CT]AG
383 CTACAG
TfiI 429 GATTC G^A[AT]TC
973 GATTC
TspRI 556 CAGTG CA[GC]TG[ACGT][ACGT]^
672 CAGTG
XmnI 67 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BcnI BsaJI BssKI Cfr13I
EarI Fnu4HI HinfI ItaI Ksp632I
MboII MnlI MseI MspA1I NciI
NlaIV Sau96I ScrFI Tru9I TseI
Tsp509I