PAPOLB ORF clone pFN21AE5002

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5002

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Clone info.

Species

human

Clone Name

pFN21AE5002 Sequence (with vector) Restriction Map

Product ID

FHC16510

ORF Sequence

DNA sequence (1908 bp)
Protein sequence (636 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5002

Symbol

PAPOLB

Alias : PAPT, TPAP

Description

poly(A) polymerase beta (testis specific)

Original Clone ID

oc95002

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1908 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 636 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS78202.1 0 99.5 PAPOLB CCDS9946.1 2.7e-203 85.8 PAPOLA CCDS1863.1 6.9e-145 63.6 PAPOLG CCDS58334.1 6.9e-96 91.8 PAPOLA CCDS58335.1 1.6e-67 81.9 PAPOLA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007012 18 364 PF04928 Poly(A) polymerase IPR002934 94 174 PF01909 Nucleotidyl transferase domain IPR007010 366 504 PF04926 Poly(A) polymerase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_064529 637 607436 99.5 100.0 XP_005267339 699 605553 85.8 98.0 XP_005267338 724 605553 85.8 98.0 NP_116021 745 605553 85.8 98.0 XP_016876425 698 605553 85.6 98.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5002 Download

>KIEE5002  1908 bp
ATGCCGTTTCCGGTGACAACCCAGGGACCACCGCAGCCGGCGCCGCCGCCGAATCGCTAC
GGCGTCTCCTCGCCTATCAGTCTAGCGGTCCCCAAGGAGACGGACTGCCTCCTCACCCAG
AGGCTAATAGAAACCCTCAGGCCCTTCGGGGTCTTCGAAGAGGAAGAGGAACTGCAGCGC
AGGATTTTAGTTTTGGACAAATTAAATAATCTGGTAAAGGAATGGATACGCGAAATCAGT
GAAAGCAAGAGTCTTCCCCAGTCTGTAATTGAAAACGTTGGAGGAAAGATTTTTACGTTT
GGCTCTTACAGATTAGGAGTACATACGAAAGGCGCAGATATTGACGCCTTGTGCGTTGCA
CCAAGTCATGTGGATCGAAGCGACTTTTTCACCTCATTCTATGCTAAACTGAAACTACAG
GAGGAAGTGAAAGATTTAAGGGCTGTCCAGGAGGCATTTGTGCCAGTTATCAAACTGTGT
TTTGATGGGATAGAGATTGATATTTTATTTGCAAGATTAGCACTACAGACTATTCCAGAA
GATTTGGACTTAAGAGATGACAGTCTACTTAAAAATTTAGACATTAGATGCATAAGAAGC
CTTAATGGTTGCCGGGTAACCGATGAAATTTTACATCTAGTGCCAAACATTGACAACTTC
AGGCTGACTCTGAGAGCCATCAAACTGTGGGCCAAGTGCCACAATATCTATTCCAATATA
TTAGGTTTCCTCGGAGGTGTTTCCTGGGCCATGCTAGTAGCAAGAACTTGTCAGCTTTAT
CCAAATGCAGTAGCGTCAACTCTTGTACGGAAATTCTTCTTGGTATTTTCAGAATGGGAA
TGGCCAAACCCAGTGTTACTGAAGGAGCCTGAAGAACGGAATCTTAATTTGCCTGTATGG
GACCCAAGAGTAAATCCCAGTGATAGGTACCATCTTATGCCTATCATCACACCAGCATAC
CCACAGCAGAACTCCACATACAACGTGTCTATTTCAACCAGGATGGTCATGATTGAGGAG
TTCAAACAGGGGCTTGCTATCACACACGAGATTTTGCTAAGTAAGGCAGAGTGGTCCAAA
CTCTTTGAAGCTCCAAGCTTCTTTCAAAAGTACAAGCATTATATTGTACTTCTGGCAAGT
GCATCAACAGAAAAACAACATTTAGAATGGGTGGGCTTGGTGGAATCAAAGATCCGAATC
CTGGTTGGGAGCTTGGAGAAGAATGAATTTATTACACTGGCACATGTGAATCCACAGTCA
TTTCCAGCACCCAAAGAAAATCCTGATATGGAAGAATTTCGTACAATGTGGGTGATTGGG
TTAGGGCTAAAAAAGCCAGATAATTCTGAAATTCTCAGCATTGATCTCACCTATGATATC
CAGTCTTTCACAGATACTGTTTATAGGCAAGCAGTGAATAGTAAGATGTTTGAGATGGGT
ATGAAAATTACTGCAGTGCATTTAAGAAGAAAGGAACTTCACCAGCTGCTGCCTCATCAT
GTGCTTCAGGACAAGAAAGCACACTCAACAGAAGGTAGAAGATTGACAGATTTGAACGAC
AGCAGCTTTGACTTGTCTGCAGGCTGTGAAAACAGCATGTCTGTGCCTTCATCTACTAGC
ACTATGAAGACAGGCCCATTGATTAGCAGTTCTCAGGGTAGAAACAGTCCTGCCTTGGCT
GTAATGACAGCATCTGTGGCTAACATACAGGCTACTGAATTTTCCTTGCAACAGGTGAAT
ACCAATGAAAGTTCAGGGGTTGCATTAAACGAAAGTATTCCTCACGCTGTCTCTCAGCCT
GCCATTTCTCCATCACCAAAGGCCATGGTCGCCAGAGTTGTTTCTTCAACATGTCTCATA
AGCCATCCAGACCTTCAGGAAACTCAGCAACAAACATATCTAATCCTA

ORF Protein

Cloned ORF protein sequence for pFN21AE5002 Download

>KIEE5002  636 aa
MPFPVTTQGPPQPAPPPNRYGVSSPISLAVPKETDCLLTQRLIETLRPFGVFEEEEELQR
RILVLDKLNNLVKEWIREISESKSLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVA
PSHVDRSDFFTSFYAKLKLQEEVKDLRAVQEAFVPVIKLCFDGIEIDILFARLALQTIPE
DLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKCHNIYSNI
LGFLGGVSWAMLVARTCQLYPNAVASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVW
DPRVNPSDRYHLMPIITPAYPQQNSTYNVSISTRMVMIEEFKQGLAITHEILLSKAEWSK
LFEAPSFFQKYKHYIVLLASASTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQS
FPAPKENPDMEEFRTMWVIGLGLKKPDNSEILSIDLTYDIQSFTDTVYRQAVNSKMFEMG
MKITAVHLRRKELHQLLPHHVLQDKKAHSTEGRRLTDLNDSSFDLSAGCENSMSVPSSTS
TMKTGPLISSSQGRNSPALAVMTASVANIQATEFSLQQVNTNESSGVALNESIPHAVSQP
AISPSPKAMVARVVSSTCLISHPDLQETQQQTYLIL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5002 Download

>pFN21AE5002  6613 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGTTTCCGGTGACAACCCAGGGACCACCGCA
GCCGGCGCCGCCGCCGAATCGCTACGGCGTCTCCTCGCCTATCAGTCTAGCGGTCCCCAA
GGAGACGGACTGCCTCCTCACCCAGAGGCTAATAGAAACCCTCAGGCCCTTCGGGGTCTT
CGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAGTTTTGGACAAATTAAATAATCTGGT
AAAGGAATGGATACGCGAAATCAGTGAAAGCAAGAGTCTTCCCCAGTCTGTAATTGAAAA
CGTTGGAGGAAAGATTTTTACGTTTGGCTCTTACAGATTAGGAGTACATACGAAAGGCGC
AGATATTGACGCCTTGTGCGTTGCACCAAGTCATGTGGATCGAAGCGACTTTTTCACCTC
ATTCTATGCTAAACTGAAACTACAGGAGGAAGTGAAAGATTTAAGGGCTGTCCAGGAGGC
ATTTGTGCCAGTTATCAAACTGTGTTTTGATGGGATAGAGATTGATATTTTATTTGCAAG
ATTAGCACTACAGACTATTCCAGAAGATTTGGACTTAAGAGATGACAGTCTACTTAAAAA
TTTAGACATTAGATGCATAAGAAGCCTTAATGGTTGCCGGGTAACCGATGAAATTTTACA
TCTAGTGCCAAACATTGACAACTTCAGGCTGACTCTGAGAGCCATCAAACTGTGGGCCAA
GTGCCACAATATCTATTCCAATATATTAGGTTTCCTCGGAGGTGTTTCCTGGGCCATGCT
AGTAGCAAGAACTTGTCAGCTTTATCCAAATGCAGTAGCGTCAACTCTTGTACGGAAATT
CTTCTTGGTATTTTCAGAATGGGAATGGCCAAACCCAGTGTTACTGAAGGAGCCTGAAGA
ACGGAATCTTAATTTGCCTGTATGGGACCCAAGAGTAAATCCCAGTGATAGGTACCATCT
TATGCCTATCATCACACCAGCATACCCACAGCAGAACTCCACATACAACGTGTCTATTTC
AACCAGGATGGTCATGATTGAGGAGTTCAAACAGGGGCTTGCTATCACACACGAGATTTT
GCTAAGTAAGGCAGAGTGGTCCAAACTCTTTGAAGCTCCAAGCTTCTTTCAAAAGTACAA
GCATTATATTGTACTTCTGGCAAGTGCATCAACAGAAAAACAACATTTAGAATGGGTGGG
CTTGGTGGAATCAAAGATCCGAATCCTGGTTGGGAGCTTGGAGAAGAATGAATTTATTAC
ACTGGCACATGTGAATCCACAGTCATTTCCAGCACCCAAAGAAAATCCTGATATGGAAGA
ATTTCGTACAATGTGGGTGATTGGGTTAGGGCTAAAAAAGCCAGATAATTCTGAAATTCT
CAGCATTGATCTCACCTATGATATCCAGTCTTTCACAGATACTGTTTATAGGCAAGCAGT
GAATAGTAAGATGTTTGAGATGGGTATGAAAATTACTGCAGTGCATTTAAGAAGAAAGGA
ACTTCACCAGCTGCTGCCTCATCATGTGCTTCAGGACAAGAAAGCACACTCAACAGAAGG
TAGAAGATTGACAGATTTGAACGACAGCAGCTTTGACTTGTCTGCAGGCTGTGAAAACAG
CATGTCTGTGCCTTCATCTACTAGCACTATGAAGACAGGCCCATTGATTAGCAGTTCTCA
GGGTAGAAACAGTCCTGCCTTGGCTGTAATGACAGCATCTGTGGCTAACATACAGGCTAC
TGAATTTTCCTTGCAACAGGTGAATACCAATGAAAGTTCAGGGGTTGCATTAAACGAAAG
TATTCCTCACGCTGTCTCTCAGCCTGCCATTTCTCCATCACCAAAGGCCATGGTCGCCAG
AGTTGTTTCTTCAACATGTCTCATAAGCCATCCAGACCTTCAGGAAACTCAGCAACAAAC
ATATCTAATCCTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT