(Linear) (Six-base) MAPSORT of: KIEE5002.seq Check: 565 from: 1 to: 1908 KIEE5002.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AccI GT'mk_AC Cuts at: 0 564 1908 Size: 564 1344 Acc65I G'GTAC_C Cuts at: 0 926 1908 Size: 926 982 AclI AA'CG_TT Cuts at: 0 275 1908 Size: 275 1633 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 880 890 1489 1858 1908 Size: 642 238 10 599 369 50 Fragments arranged by size: 642 599 369 238 50 10 AflII C'TTAA_G Cuts at: 0 549 1908 Size: 549 1359 AflIII A'CryG_T Cuts at: 0 983 1242 1849 1908 Size: 983 259 607 59 Fragments arranged by size: 983 607 259 59 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1618 1908 Size: 1618 290 AlwNI CAG_nnn'CTG Cuts at: 0 1396 1488 1693 1908 Size: 1396 92 205 215 Fragments arranged by size: 1396 215 205 92 ApoI r'AATT_y Cuts at: 0 573 626 811 1225 1294 1349 1717 1908 Size: 573 53 185 414 69 55 368 191 Fragments arranged by size: 573 414 368 191 185 69 55 53 BanI G'GyrC_C Cuts at: 0 39 926 1908 Size: 39 887 982 Fragments arranged by size: 982 887 39 BbeI G_GCGC'C Cuts at: 0 43 1908 Size: 43 1865 BbsI GAAGACnn'nnnn_ Cuts at: 0 144 244 1633 1908 Size: 144 100 1389 275 Fragments arranged by size: 1389 275 144 100 BciVI GTATCCnnnnn_n' Cuts at: 0 218 1908 Size: 218 1690 BfrBI ATG'CAT Cuts at: 0 590 1908 Size: 590 1318 BmrI ACTGGGnnnn_n' Cuts at: 0 252 844 911 1908 Size: 252 592 67 997 Fragments arranged by size: 997 592 252 67 BsaBI GATnn'nnATC Cuts at: 0 1195 1908 Size: 1195 713 BsaHI Gr'CG_yC Cuts at: 0 40 62 344 1908 Size: 40 22 282 1564 Fragments arranged by size: 1564 282 40 22 BsaWI w'CCGG_w Cuts at: 0 9 1908 Size: 9 1899 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 49 79 1908 Size: 49 30 1829 Fragments arranged by size: 1829 49 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 57 100 1030 1908 Size: 57 43 930 878 Fragments arranged by size: 930 878 57 43 BsmBI CGTCTCn'nnnn_ Cuts at: 0 69 91 1908 Size: 69 22 1817 Fragments arranged by size: 1817 69 22 BspHI T'CATG_A Cuts at: 0 1007 1908 Size: 1007 901 BsrFI r'CCGG_y Cuts at: 0 36 1908 Size: 36 1872 BssSI C'ACGA_G Cuts at: 0 1045 1908 Size: 1045 863 BstBI TT'CG_AA Cuts at: 0 155 1908 Size: 155 1753 BstEII G'GTnAC_C Cuts at: 0 615 1908 Size: 615 1293 BstYI r'GATC_y Cuts at: 0 1190 1908 Size: 1190 718 Bsu36I CC'TnA_GG Cuts at: 0 136 1908 Size: 136 1772 BtgI C'CryG_G Cuts at: 0 1823 1908 Size: 1823 85 BtsI GCAGTG_nn' Cuts at: 0 1418 1460 1908 Size: 1418 42 448 Fragments arranged by size: 1418 448 42 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 350 385 1908 Size: 350 35 1523 Fragments arranged by size: 1523 350 35 EaeI y'GGCC_r Cuts at: 0 841 1908 Size: 841 1067 EarI CTCTTCn'nnn_ Cuts at: 0 152 158 1908 Size: 152 6 1750 Fragments arranged by size: 1750 152 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 880 890 1489 1858 1908 Size: 642 238 10 599 369 50 Fragments arranged by size: 642 599 369 238 50 10 EcoO109I rG'GnC_Cy Cuts at: 0 140 900 1908 Size: 140 760 1008 Fragments arranged by size: 1008 760 140 EcoRV GAT'ATC Cuts at: 0 1377 1908 Size: 1377 531 HaeII r_GCGC'y Cuts at: 0 43 1908 Size: 43 1865 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1366 1398 1908 Size: 1366 32 510 Fragments arranged by size: 1366 510 32 HincII GTy'rAC Cuts at: 0 797 1908 Size: 797 1111 HindIII A'AGCT_T Cuts at: 0 1095 1908 Size: 1095 813 KasI G'GCGC_C Cuts at: 0 39 1908 Size: 39 1869 KpnI G_GTAC'C Cuts at: 0 930 1908 Size: 930 978 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 258 1908 Size: 258 1650 MscI TGG'CCA Cuts at: 0 843 1908 Size: 843 1065 MslI CAynn'nnrTG Cuts at: 0 1600 1908 Size: 1600 308 MspA1I CmG'CkG Cuts at: 0 1485 1908 Size: 1485 423 NaeI GCC'GGC Cuts at: 0 38 1908 Size: 38 1870 NarI GG'CG_CC Cuts at: 0 40 1908 Size: 40 1868 NcoI C'CATG_G Cuts at: 0 1823 1908 Size: 1823 85 NgoMIV G'CCGG_C Cuts at: 0 36 1908 Size: 36 1872 NsiI A_TGCA'T Cuts at: 0 592 1908 Size: 592 1316 NspI r_CATG'y Cuts at: 0 1246 1599 1853 1908 Size: 1246 353 254 55 Fragments arranged by size: 1246 353 254 55 PasI CC'CwG_GG Cuts at: 0 21 1908 Size: 21 1887 PciI A'CATG_T Cuts at: 0 1242 1849 1908 Size: 1242 607 59 Fragments arranged by size: 1242 607 59 PfoI T'CCnGG_A Cuts at: 0 446 1908 Size: 446 1462 PpuMI rG'GwC_Cy Cuts at: 0 900 1908 Size: 900 1008 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 961 993 1908 Size: 961 32 915 Fragments arranged by size: 961 915 32 PstI C_TGCA'G Cuts at: 0 176 1455 1581 1908 Size: 176 1279 126 327 Fragments arranged by size: 1279 327 176 126 PvuII CAG'CTG Cuts at: 0 1485 1908 Size: 1485 423 SanDI GG'GwC_CC Cuts at: 0 900 1908 Size: 900 1008 SfcI C'TryA_G Cuts at: 0 172 415 523 1451 1577 1908 Size: 172 243 108 928 126 331 Fragments arranged by size: 928 331 243 172 126 108 SfoI GGC'GCC Cuts at: 0 41 1908 Size: 41 1867 SmlI C'TyrA_G Cuts at: 0 549 1908 Size: 549 1359 StyI C'CwwG_G Cuts at: 0 92 1673 1823 1908 Size: 92 1581 150 85 Fragments arranged by size: 1581 150 92 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 130 1158 1284 1299 1908 Size: 130 1028 126 15 609 Fragments arranged by size: 1028 609 130 126 15 TatI w'GTAC_w Cuts at: 0 318 1109 1125 1908 Size: 318 791 16 783 Fragments arranged by size: 791 783 318 16 XmnI GAAnn'nnTTC Cuts at: 0 814 1295 1775 1908 Size: 814 481 480 133 Fragments arranged by size: 814 481 480 133 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI ApoI BanI BbeI BbsI BciVI BfrBI BmrI BsaBI BsaHI BsaWI BsaXI BseRI BsmBI BspHI BsrFI BssSI BstBI BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI PpuMI PsrI PstI PvuII SanDI SfcI SfoI SmlI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII AfeI AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstXI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI MfeI MluI NdeI NheI NotI NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI