################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BclI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI FauI FbaI FseI FspI HpaI KspI MfeI MluI MroI MunI NdeI NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 926 GGTACC G^GTACC AccI 563 GTCTAC GT^[AC][GT]AC AccII 229 CGCG CG^CG AclI 274 AACGTT AA^CGTT AflII 549 CTTAAG C^TTAAG AocI 135 CCTCAGG CC^T[ACGT]AGG Asp718I 926 GGTACC G^GTACC BalI 841 TGGCCA TGG^CCA BbeI 39 GGCGCC GGCGC^C BciVI 224 GGATAC GTATCC(6/5) BcnI 612 CCGGG CC^[GC]GG BfrI 549 CTTAAG C^TTAAG BsaBI 1191 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 9 TCCGGT [AT]^CCGG[AT] BspHI 1007 TCATGA T^CATGA BsrFI 36 GCCGGC [GA]^CCGG[CT] BssSI 1045 CACGAG CACGAG(-5/-1) BstBI 154 TTCGAA TT^CGAA BstEII 615 GGTAACC G^GT[ACGT]ACC BstPI 615 GGTAACC G^GT[ACGT]ACC BstUI 229 CGCG CG^CG BstYI 1190 AGATCC [GA]^GATC[CT] Bsu36I 135 CCTCAGG CC^T[ACGT]AGG Cfr10I 36 GCCGGC [GA]^CCGG[CT] DsaI 1823 CCATGG C^C[GA][CT]GG EaeI 841 TGGCCA [CT]^GGCC[GA] Eco81I 135 CCTCAGG CC^T[ACGT]AGG EcoO65I 615 GGTAACC G^GT[ACGT]ACC EcoRV 1375 GATATC GAT^ATC EcoT22I 588 ATGCAT ATGCA^T HaeII 39 GGCGCC [GA]GCGC^[CT] HincII 795 GTCAAC GT[CT]^[GA]AC HindII 795 GTCAAC GT[CT]^[GA]AC HindIII 1095 AAGCTT A^AGCTT KasI 39 GGCGCC G^GCGCC KpnI 926 GGTACC GGTAC^C MamI 1191 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1190 AGATCC [GA]^GATC[CT] MluNI 841 TGGCCA TGG^CCA MscI 841 TGGCCA TGG^CCA MslI 1596 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1483 CAGCTG C[AC]G^C[GT]G MvnI 229 CGCG CG^CG NaeI 36 GCCGGC GCC^GGC NarI 39 GGCGCC GG^CGCC NciI 612 CCGGG CC^[GC]GG NcoI 1823 CCATGG C^CATGG NgoMIV 36 GCCGGC G^CCGGC NsiI 588 ATGCAT ATGCA^T NspV 154 TTCGAA TT^CGAA PpuMI 899 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 274 AACGTT AA^CGTT PvuII 1483 CAGCTG CAG^CTG RcaI 1007 TCATGA T^CATGA SfoI 39 GGCGCC GGC^GCC SfuI 154 TTCGAA TT^CGAA SmlI 549 CTTAAG C^T[CT][GA]AG Tsp45I 13 GTGAC ^GT[GC]AC XhoII 1190 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwI 1191 GATCC GGATC(4/5) 372 GGATC BanI 39 GGCGCC G^G[CT][GA]CC 926 GGTACC BsiYI 917 CCAGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1194 CCGAATCCTGG BslI 917 CCAGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1194 CCGAATCCTGG BsmBI 63 CGTCTC CGTCTC(1/5) 97 GAGACG BspLU11I 1242 ACATGT A^CATGT 1849 ACATGT BstF5I 1864 CATCC GGATG(2/0) 1001 GGATG DraII 139 AGGCCCT [GA]G^G[ACGT]CC[CT] 899 GGGACCC EarI 157 GAAGAG CTCTTC(1/4) 163 GAAGAG EcoO109I 139 AGGCCCT [GA]G^G[ACGT]CC[CT] 899 GGGACCC FokI 1864 CATCC GGATG(9/13) 1001 GGATG Ksp632I 157 GAAGAG CTCTTC(1/4) 163 GAAGAG PleI 666 GACTC GAGTC(4/5) 249 GAGTC TaqI 155 TCGA T^CGA 375 TCGA TthHB8I 155 TCGA T^CGA 375 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwNI ApoI Asp700I AvaII BbsI BbvI BfaI BpuAI BsaHI BsaJI BseRI BsmAI BsmFI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XmnI